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John Hassell,  Ph.D.
Professor, Molecular Medicine


Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

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Contacts
Email: jhassell@health.usf.edu
Phone: (813) 974-7881
Office: MDC, 3014
Mailing address University of South Florida 12901 Bruce B Downs Blvd., MDC 7
Tampa, FL 33612-4799
 
Education
  • PHD, BIOLOGY, University Of Connecticut , STORRS, United States - 1972.
  • BA, Biology, Central Connecticut State ,USA - 1966.
  •  
    Interdisciplinary & Emerging Signature Programs
    Biomedical Engineering & Nanomedicine.
     
    Research Summary

    My research has focused on identifying the proteoglycans present in cartilage, basement membrane and cornea and determining their role in disease and development. My current research interest is in the extra cellular matrix (ECM) made by keratocytes during wound healing. The corneal stroma consists of an extensive extra cellular ECM interspersed with keratocytes. This ECM is uniquely transparent due to the small uniform diameter and spacing of the collagen fibrils. Wounds to the cornea readily heal but the stomal keratocytes can often produce opaque scars that cause blindness. Stromal wound healing is currently thought to consist of three major phases. First, the injured keratocytes as well as some keratocytes flanking the wound undergo apoptosis. Second, some of the surviving keratocytes are activated to proliferate and become hypercellular myofibroblasts (myofibroblasts that produce little ECM, resulting in densely packed cells). The hypercellular myofibroblasts can remain as such and produce a hypercellular scar, or they can be stimulated to produce one of two kinds of ECM: 1) a normal collagenous matrix that restores transparency, 2) a fibrocollagenous matrix that produces an opaque scar. We have found, by using keratocytes in culture that the hypercellular and collagenous phases of wound healing can be reproduced in vitro by specific growth factors. FGF-2 and TGF-beta together would produce the hypercellular phase, IGF-1 alone would produce the collagenous phase and TGF-beta alone would produce the fibrocollagenous phase. We are now using these growth factors in tissue engineering studies to produce a stromal construct for use in corneal transplantation.

     


    Selected Publications
  • Kane, BP.Jester, JV.Huang, J.Wahlert, A.Hassell, JR. IGF-II and collagen expression by keratocytes during postnatal development. Experimental eye research. (): , 2009.
  • Etheredge, L.Kane, B.Hassell, JR. The effect of growth factor signaling on keratocytes in vitro and its relationship to the phases of stromal wound repair. Investigative ophthalmology & visual science. (): , 2009.
  • Hassell, JR.Kane, BP.Etheredge, LT.Valkov, N.Birk, DE. Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose. Experimental eye research. 87(6): 604-11, 2008.
  • Musselmann, K.Kane, BP.Alexandrou, B.Hassell, JR. IGF-II is present in bovine corneal stroma and activates keratocytes to proliferate in vitro. Experimental eye research. 86(3): 506-11, 2008.
  • Smith, SM.West, LA.Govindraj, P.Zhang, X.Ornitz, DM.Hassell, JR. Heparan and chondroitin sulfate on growth plate perlecan mediate binding and delivery of FGF-2 to FGF receptors. Matrix biology : journal of the International Society for Matrix Biology. 26(3): 175-84, 2007.
  • Smith, SM.West, LA.Hassell, JR. The core protein of growth plate perlecan binds FGF-18 and alters its mitogenic effect on chondrocytes. Archives of biochemistry and biophysics. 468(2): 244-51, 2007.
  • West, L.Govindraj, P.Koob, TJ.Hassell, JR. Changes in perlecan during chondrocyte differentiation in the fetal bovine rib growth plate. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 24(6): 1317-26, 2006.
  • Govindraj, P.West, L.Smith, S.Hassell, JR. Modulation of FGF-2 binding to chondrocytes from the developing growth plate by perlecan. Matrix biology : journal of the International Society for Matrix Biology. 25(4): 232-9, 2006.
  • Smith, S.Hassell, JR. Focus on molecules: perlecan (HSPG2). Experimental eye research. 83(3): 471-2, 2006.
  • Musselmann, K.Hassell, JR. Focus on molecules: CHST6 (carbohydrate sulfotransferase 6; corneal N-acetylglucosamine-6-sulfotransferase). Experimental eye research. 83(4): 707-8, 2006.
  • Musselmann, K.Kane, B.Alexandrou, B.Hassell, JR. Stimulation of collagen synthesis by insulin and proteoglycan accumulation by ascorbate in bovine keratocytes in vitro. Investigative ophthalmology & visual science. 47(12): 5260-6, 2006.
  • Musselmann, K.Alexandrou, B.Kane, B.Hassell, JR. Maintenance of the keratocyte phenotype during cell proliferation stimulated by insulin. The Journal of biological chemistry. 280(38): 32634-9, 2005.
  • Fukuchi, K.Hart, M.Yan, Z.Hassell, JR.Li, L. Transgenic mice overexpressing both amyloid beta-protein and perlecan in pancreatic acinar cells. Histology and histopathology. 19(3): 845-52, 2004.
  • Musselmann, K.Kane, BP.Hassell, JR. Isolation of a putative keratocyte activating factor from the corneal stroma. Experimental eye research. 77(3): 273-9, 2003.
  • Liu, CY.Birk, DE.Hassell, JR.Kane, B.Kao, WW. Keratocan-deficient mice display alterations in corneal structure. The Journal of biological chemistry. 278(24): 21672-7, 2003.
  • Berryhill, BL.Kane, B.Stramer, BM.Fini, ME.Hassell, JR. Increased SPARC accumulation during corneal repair. Experimental eye research. 77(1): 85-92, 2003.
  • Arikawa-Hirasawa, E.Le, AH.Nishino, I.Nonaka, I.Ho, NC.Francomano, CA.Govindraj, P.Hassell, JR.Devaney, JM.Spranger, J.Stevenson, RE.Iannaccone, S.Dalakas, MC.Yamada, Y. Structural and functional mutations of the perlecan gene cause Schwartz-Jampel syndrome, with myotonic myopathy and chondrodysplasia. American journal of human genetics. 70(5): 1368-75, 2002.
  • Govindraj, P.West, L.Koob, TJ.Neame, P.Doege, K.Hassell, JR. Isolation and identification of the major heparan sulfate proteoglycans in the developing bovine rib growth plate. The Journal of biological chemistry. 277(22): 19461-9, 2002.
  • Berryhill, BL.Kader, R.Kane, B.Birk, DE.Feng, J.Hassell, JR. Partial restoration of the keratocyte phenotype to bovine keratocytes made fibroblastic by serum. Investigative ophthalmology & visual science. 43(11): 3416-21, 2002.
  • Pandit, SG.Govindraj, P.Sasse, J.Neame, PJ.Hassell, JR. The fibroblast growth factor receptor, FGFR3, forms gradients of intact and degraded protein across the growth plate of developing bovine ribs. The Biochemical journal. 361(Pt 2): 231-41, 2002.
  • Arikawa-Hirasawa, E.Wilcox, WR.Le, AH.Silverman, N.Govindraj, P.Hassell, JR.Yamada, Y. Dyssegmental dysplasia, Silverman-Handmaker type, is caused by functional null mutations of the perlecan gene. Nature genetics. 27(4): 431-4, 2001.
  • Hart, M.Li, L.Tokunaga, T.Lindsey, JR.Hassell, JR.Snow, AD.Fukuchi, K. Overproduction of perlecan core protein in cultured cells and transgenic mice. The Journal of pathology. 194(2): 262-9, 2001.
  • Berryhill, BL.Beales, MP.Hassell, JR. Production of prostaglandin D synthase as a keratan sulfate proteoglycan by cultured bovine keratocytes. Investigative ophthalmology & visual science. 42(6): 1201-7, 2001.
  • Corpuz, LM.Dunlevy, JR.Hassell, JR.Conrad, AH.Conrad, GW. Molecular cloning and relative tissue expression of decorin and lumican in embryonic quail cornea. Matrix biology : journal of the International Society for Matrix Biology. 19(7): 699-704, 2000.
  • Dunlevy, JR.Beales, MP.Berryhill, BL.Cornuet, PK.Hassell, JR. Expression of the keratan sulfate proteoglycans lumican, keratocan and osteoglycin/mimecan during chick corneal development. Experimental eye research. 70(3): 349-62, 2000.
  • Corpuz, LM.Dunlevy, JR.Hassell, JR.Conrad, AH.Conrad, GW. Molecular cloning and relative tissue expression of keratocan and mimecan in embryonic quail cornea. Matrix biology : journal of the International Society for Matrix Biology. 19(7): 693-8, 2000.
  • Chakravarti, S.Petroll, WM.Hassell, JR.Jester, JV.Lass, JH.Paul, J.Birk, DE. Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma. Investigative ophthalmology & visual science. 41(11): 3365-73, 2000.
  • Neame, PJ.Kay, CJ.McQuillan, DJ.Beales, MP.Hassell, JR. Independent modulation of collagen fibrillogenesis by decorin and lumican. Cellular and molecular life sciences : CMLS. 57(5): 859-63, 2000.
  • Tapanadechopone, P.Hassell, JR.Rigatti, B.Couchman, JR. Localization of glycosaminoglycan substitution sites on domain V of mouse perlecan. Biochemical and biophysical research communications. 265(3): 680-90, 1999.
  • Dunlevy, JR.Berryhill, BL.Vergnes, JP.SundarRaj, N.Hassell, JR. Cloning, chromosomal localization, and characterization of cDNA from a novel gene, SH3BP4, expressed by human corneal fibroblasts. Genomics. 62(3): 519-24, 1999.
  • Villar, MJ.Hassell, JR.Brandan, E. Interaction of skeletal muscle cells with collagen type IV is mediated by perlecan associated with the cell surface. Journal of cellular biochemistry. 75(4): 665-74, 1999.
  • Beales, MP.Funderburgh, JL.Jester, JV.Hassell, JR. Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of the keratocyte phenotype in culture. Investigative ophthalmology & visual science. 40(8): 1658-63, 1999.
  • Arikawa-Hirasawa, E.Watanabe, H.Takami, H.Hassell, JR.Yamada, Y. Perlecan is essential for cartilage and cephalic development. Nature genetics. 23(3): 354-8, 1999.
  • Dunlevy, JR.Neame, PJ.Vergnes, JP.Hassell, JR. Identification of the N-linked oligosaccharide sites in chick corneal lumican and keratocan that receive keratan sulfate. The Journal of biological chemistry. 273(16): 9615-21, 1998.
  • Dunlevy, JR.Chakravarti, S.Gyalzen, P.Vergnes, JP.Hassell, JR. Cloning and chromosomal localization of mouse keratocan, a corneal keratan sulfate proteoglycan. Mammalian genome : official journal of the International Mammalian Genome Society. 9(4): 316-9, 1998.
  • Hassell, JR.Rada, J.Cornuet, P.Vergnes, JP.Kinchington, PR. Gene structure of chick lumican and identification of the first exon. Biochimica et biophysica acta. 1397(2): 119-25, 1998.
  • Peng, HB.Ali, AA.Daggett, DF.Rauvala, H.Hassell, JR.Smalheiser, NR. The relationship between perlecan and dystroglycan and its implication in the formation of the neuromuscular junction. Cell adhesion and communication. 5(6): 475-89, 1998.
  • Sundarraj, N.Fite, D.Belak, R.Sundarraj, S.Rada, J.Okamoto, S.Hassell, J. Proteoglycan distribution during healing of corneal stromal wounds in chick. Experimental eye research. 67(4): 433-42, 1998.
  • Larraín, J.Alvarez, J.Hassell, JR.Brandan, E. Expression of perlecan, a proteoglycan that binds myogenic inhibitory basic fibroblast growth factor, is down regulated during skeletal muscle differentiation. Experimental cell research. 234(2): 405-12, 1997.
  • Dolan, M.Horchar, T.Rigatti, B.Hassell, JR. Identification of sites in domain I of perlecan that regulate heparan sulfate synthesis. The Journal of biological chemistry. 272(7): 4316-22, 1997.
  • Hassell, JR.Klintworth, GK. Serum sulfotransferase levels in patients with macular corneal dystrophy type I. Archives of ophthalmology. 115(11): 1419-21, 1997.
  • Rada, JA.Fini, ME.Hassell, JR. Regionalized growth patterns of young chicken corneas. Investigative ophthalmology & visual science. 37(10): 2060-7, 1996.
  • Couchman, JR.Ljubimov, AV.Sthanam, M.Horchar, T.Hassell, JR. Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 43(9): 955-63, 1995.
  • SundarRaj, N.Fite, D.Ledbetter, S.Chakravarti, S.Hassell, JR. Perlecan is a component of cartilage matrix and promotes chondrocyte attachment. Journal of cell science. 108 ( Pt 7)(): 2663-72, 1995.
  • Chakravarti, S.Stallings, RL.SundarRaj, N.Cornuet, PK.Hassell, JR. Primary structure of human lumican (keratan sulfate proteoglycan) and localization of the gene (LUM) to chromosome 12q21.3-q22. Genomics. 27(3): 481-8, 1995.
  • Chakravarti, S.Horchar, T.Jefferson, B.Laurie, GW.Hassell, JR. Recombinant domain III of perlecan promotes cell attachment through its RGDS sequence. The Journal of biological chemistry. 270(1): 404-9, 1995.
  • Okamoto, S.Ohji, M.Kosaku, K.Sundarraj, N.Hassell, JR.Thoft, RA.Pipas, JM. Characterization of immortalized rabbit corneal epithelial cells with SV40 large T antigen. Japanese journal of ophthalmology. 39(4): 323-33, 1995.
  • Ohji, M.SundarRaj, N.Hassell, JR.Thoft, RA. Basement membrane synthesis by human corneal epithelial cells in vitro. Investigative ophthalmology & visual science. 35(2): 479-85, 1994.
  • Cornuet, PK.Blochberger, TC.Hassell, JR. Molecular polymorphism of lumican during corneal development. Investigative ophthalmology & visual science. 35(3): 870-7, 1994.
  • Mandarino, LJ.Finlayson, J.Hassell, JR. High glucose downregulates glucose transport activity in retinal capillary pericytes but not endothelial cells. Investigative ophthalmology & visual science. 35(3): 964-72, 1994.
  • Funderburgh, JL.Funderburgh, ML.Brown, SJ.Vergnes, JP.Hassell, JR.Mann, MM.Conrad, GW. Sequence and structural implications of a bovine corneal keratan sulfate proteoglycan core protein. Protein 37B represents bovine lumican and proteins 37A and 25 are unique. The Journal of biological chemistry. 268(16): 11874-80, 1993.
  • Rada, JA.Cornuet, PK.Hassell, JR. Regulation of corneal collagen fibrillogenesis in vitro by corneal proteoglycan (lumican and decorin) core proteins. Experimental eye research. 56(6): 635-48, 1993.
  • Chakravarti, S.Hassell, JR.Phillips, SL. Perlecan gene expression precedes laminin gene expression during differentiation of F9 embryonal carcinoma cells. Developmental dynamics : an official publication of the American Association of Anatomists. 197(2): 107-14, 1993.
  • Noonan, DM.Hassell, JR. Perlecan, the large low-density proteoglycan of basement membranes: structure and variant forms. Kidney international. 43(1): 53-60, 1993.
  • Rescan, PY.Loréal, O.Hassell, JR.Yamada, Y.Guillouzo, A.Clément, B. Distribution and origin of the basement membrane component perlecan in rat liver and primary hepatocyte culture. The American journal of pathology. 142(1): 199-208, 1993.
  • Li, W.Vergnes, JP.Cornuet, PK.Hassell, JR. cDNA clone to chick corneal chondroitin/dermatan sulfate proteoglycan reveals identity to decorin. Archives of biochemistry and biophysics. 296(1): 190-7, 1992.
  • Rada, JA.McFarland, AL.Cornuet, PK.Hassell, JR. Proteoglycan synthesis by scleral chondrocytes is modulated by a vision dependent mechanism. Current eye research. 11(8): 767-82, 1992.
  • Blochberger, TC.Cornuet, PK.Hassell, JR. Isolation and partial characterization of lumican and decorin from adult chicken corneas. A keratan sulfate-containing isoform of decorin is developmentally regulated. The Journal of biological chemistry. 267(29): 20613-9, 1992.
  • Hassell, JR.Schrecengost, PK.Rada, JA.SundarRaj, N.Sossi, G.Thoft, RA. Biosynthesis of stromal matrix proteoglycans and basement membrane components by human corneal fibroblasts. Investigative ophthalmology & visual science. 33(3): 547-57, 1992.
  • Schrecengost, PK.Blochberger, TC.Hassell, JR. Identification of chick corneal keratan sulfate proteoglycan precursor protein in whole corneas and in cultured corneal fibroblasts. Archives of biochemistry and biophysics. 292(1): 54-61, 1992.
  • Blochberger, TC.Vergnes, JP.Hempel, J.Hassell, JR. cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine. The Journal of biological chemistry. 267(1): 347-52, 1992.
  • Danielson, KG.Martinez-Hernandez, A.Hassell, JR.Iozzo, RV. Establishment of a cell line from the EHS tumor: biosynthesis of basement membrane constituents and characterization of a hybrid proteoglycan containing heparan and chondroitin sulfate chains. Matrix (Stuttgart, Germany). 12(1): 22-35, 1992.
  • Noonan, DM.Fulle, A.Valente, P.Cai, S.Horigan, E.Sasaki, M.Yamada, Y.Hassell, JR. The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule. The Journal of biological chemistry. 266(34): 22939-47, 1991.
  • Jost, CJ.Funderburgh, JL.Mann, M.Hassell, JR.Conrad, GW. Cell-free translation and characterization of corneal keratan sulfate proteoglycan core proteins. The Journal of biological chemistry. 266(20): 13336-41, 1991.
  • Dodge, GR.Kovalszky, I.Chu, ML.Hassell, JR.McBride, OW.Yi, HF.Iozzo, RV. Heparan sulfate proteoglycan of human colon: partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1. Genomics. 10(3): 673-80, 1991.
  • Rada, JA.Thoft, RA.Hassell, JR. Increased aggrecan (cartilage proteoglycan) production in the sclera of myopic chicks. Developmental biology. 147(2): 303-12, 1991.
  • Chakravarti, S.Phillips, SL.Hassell, JR. Assignment of the perlecan (heparan sulfate proteoglycan) gene to mouse chromosome 4. Mammalian genome : official journal of the International Mammalian Genome Society. 1(4): 270-2, 1991.
  • Midura, RJ.Hascall, VC.MacCallum, DK.Meyer, RF.Thonar, EJ.Hassell, JR.Smith, CF.Klintworth, GK. Proteoglycan biosynthesis by human corneas from patients with types 1 and 2 macular corneal dystrophy. The Journal of biological chemistry. 265(26): 15947-55, 1990.
  • Dodge, GR.Kovalszky, I.Hassell, JR.Iozzo, RV. Transforming growth factor beta alters the expression of heparan sulfate proteoglycan in human colon carcinoma cells. The Journal of biological chemistry. 265(29): 18023-9, 1990.
  • Ledbetter, S.Copeland, EJ.Noonan, D.Vogeli, G.Hassell, JR. Altered steady-state mRNA levels of basement membrane proteins in diabetic mouse kidneys and thromboxane synthase inhibition. Diabetes. 39(2): 196-203, 1990.
  • Mukhopadhyay, NK.Shome, K.Saha, AK.Hassell, JR.Glew, RH. Heparin binds to Leishmania donovani promastigotes and inhibits protein phosphorylation. The Biochemical journal. 264(2): 517-25, 1989.
  • Clément, B.Segui-Real, B.Hassell, JR.Martin, GR.Yamada, Y. Identification of a cell surface-binding protein for the core protein of the basement membrane proteoglycan. The Journal of biological chemistry. 264(21): 12467-71, 1989.
  • Grant, DS.Leblond, CP.Kleinman, HK.Inoue, S.Hassell, JR. The incubation of laminin, collagen IV, and heparan sulfate proteoglycan at 35 degrees C yields basement membrane-like structures. The Journal of cell biology. 108(4): 1567-74, 1989.
  • Iozzo, RV.Hassell, JR. Identification of the precursor protein for the heparan sulfate proteoglycan of human colon carcinoma cells and its post-translational modifications. Archives of biochemistry and biophysics. 269(1): 239-49, 1989.
  • Laurie, GW.Inoue, S.Bing, JT.Hassell, JR. Visualization of the large heparan sulfate proteoglycan from basement membrane. The American journal of anatomy. 181(3): 320-6, 1988.
  • Klein, DJ.Brown, DM.Oegema, TR.Brenchley, PE.Anderson, JC.Dickinson, MA.Horigan, EA.Hassell, JR. Glomerular basement membrane proteoglycans are derived from a large precursor. The Journal of cell biology. 106(3): 963-70, 1988.
  • Noonan, DM.Horigan, EA.Ledbetter, SR.Vogeli, G.Sasaki, M.Yamada, Y.Hassell, JR. Identification of cDNA clones encoding different domains of the basement membrane heparan sulfate proteoglycan. The Journal of biological chemistry. 263(31): 16379-87, 1988.
  • Horton, WE.Cleveland, J.Rapp, U.Nemuth, G.Bolander, M.Doege, K.Yamada, Y.Hassell, JR. An established rat cell line expressing chondrocyte properties. Experimental cell research. 178(2): 457-68, 1988.
  • Newsome, DA.Pfeffer, BA.Hewitt, AT.Robey, PG.Hassell, JR. Detection of extracellular matrix molecules synthesized in vitro by monkey and human retinal pigment epithelium: influence of donor age and multiple passages. Experimental eye research. 46(3): 305-21, 1988.
  • Ledbetter, SR.Wagner, CW.Martin, GR.Rohrbach, DH.Hassell, JR. Response of diabetic basement membrane--producing cells to glucose and insulin. Diabetes. 36(9): 1029-34, 1987.
  • Horton, WE.Yamada, Y.Hassell, JR. Retinoic acid rapidly reduces cartilage matrix synthesis by altering gene transcription in chondrocytes. Developmental biology. 123(2): 508-16, 1987.
  • Ledbetter, SR.Fisher, LW.Hassell, JR. Domain structure of the basement membrane heparan sulfate proteoglycan. Biochemistry. 26(4): 988-95, 1987.
  • Doege, K.Sasaki, M.Horigan, E.Hassell, JR.Yamada, Y. Complete primary structure of the rat cartilage proteoglycan core protein deduced from cDNA clones. The Journal of biological chemistry. 262(36): 17757-67, 1987.
  • Horton, W.Miyashita, T.Kohno, K.Hassell, JR.Yamada, Y. Identification of a phenotype-specific enhancer in the first intron of the rat collagen II gene. Proceedings of the National Academy of Sciences of the United States of America. 84(24): 8864-8, 1987.
  • Singer, II.Scott, S.Kawka, DW.Hassell, JR. Extracellular matrix fibers containing fibronectin and basement membrane heparan sulfate proteoglycan coalign with focal contacts and microfilament bundles in stationary fibroblasts. Experimental cell research. 173(2): 558-71, 1987.
  • Newsome, DA.Hewitt, AT.Huh, W.Robey, PG.Hassell, JR. Detection of specific extracellular matrix molecules in drusen, Bruch's membrane, and ciliary body. American journal of ophthalmology. 104(4): 373-81, 1987.
  • Doege, K.Hassell, JR.Caterson, B.Yamada, Y. Link protein cDNA sequence reveals a tandemly repeated protein structure. Proceedings of the National Academy of Sciences of the United States of America. 83(11): 3761-5, 1986.
  • Klintworth, GK.Meyer, R.Dennis, R.Hewitt, AT.Stock, EL.Lenz, ME.Hassell, JR.Stark, WJ.Kuettner, KE.Thonar, EJ. Macular corneal dystrophy. Lack of keratan sulfate in serum and cornea. Ophthalmic paediatrics and genetics. 7(3): 139-43, 1986.
  • Ledbetter, SR.Hassell, JR. beta-D-xyloside-mediated alteration in the synthesis of basement membrane proteoglycan. Archives of biochemistry and biophysics. 246(1): 403-10, 1986.
  • Kleinman, HK.McGarvey, ML.Hassell, JR.Star, VL.Cannon, FB.Laurie, GW.Martin, GR. Basement membrane complexes with biological activity. Biochemistry. 25(2): 312-8, 1986.
  • Hassell, JR.Kimura, JH.Hascall, VC. Proteoglycan core protein families. Annual review of biochemistry. 55(): 539-67, 1986.
  • Laurie, GW.Bing, JT.Kleinman, HK.Hassell, JR.Aumailley, M.Martin, GR.Feldmann, RJ. Localization of binding sites for laminin, heparan sulfate proteoglycan and fibronectin on basement membrane (type IV) collagen. Journal of molecular biology. 189(1): 205-16, 1986.
  • Thonar, EJ.Meyer, RF.Dennis, RF.Lenz, ME.Maldonado, B.Hassell, JR.Hewitt, AT.Stark, WJ.Stock, EL.Kuettner, KE. Absence of normal keratan sulfate in the blood of patients with macular corneal dystrophy. American journal of ophthalmology. 102(5): 561-9, 1986.
  • Tyree, B.Hassell, JR.Hascall, VC. Altered synthesis of heparan sulfate proteoglycans at low sulfate concentration. Archives of biochemistry and biophysics. 250(1): 202-10, 1986.
  • Doege, K.Fernandez, P.Hassell, JR.Sasaki, M.Yamada, Y. Partial cDNA sequence encoding a globular domain at the C terminus of the rat cartilage proteoglycan. The Journal of biological chemistry. 261(18): 8108-11, 1986.
  • Horton, W.Hassell, JR. Independence of cell shape and loss of cartilage matrix production during retinoic acid treatment of cultured chondrocytes. Developmental biology. 115(2): 392-7, 1986.
  • Kleinman, HK.Cannon, FB.Laurie, GW.Hassell, JR.Aumailley, M.Terranova, VP.Martin, GR.DuBois-Dalcq, M. Biological activities of laminin. Journal of cellular biochemistry. 27(4): 317-25, 1985.
  • Hassell, JR.Horigan, EA.Mosley, GL.Ledbetter, SR.Kleinman, HK.Chandrasekhar, S. Appearance of basement membrane components during the differentiation of limb bud mesenchyme cells in culture. Progress in clinical and biological research. 171(): 75-86, 1985.
  • Hassell, JR.Leyshon, WC.Ledbetter, SR.Tyree, B.Suzuki, S.Kato, M.Kimata, K.Kleinman, HK. Isolation of two forms of basement membrane proteoglycans. The Journal of biological chemistry. 260(13): 8098-105, 1985.
  • Ledbetter, SR.Tyree, B.Hassell, JR.Horigan, EA. Identification of the precursor protein to basement membrane heparan sulfate proteoglycans. The Journal of biological chemistry. 260(13): 8106-113, 1985.
  • Wewer, UM.Albrechtsen, R.Hassell, JR. Heparan sulfate proteoglycans made by different basement-membrane-producing tumors have immunological and structural similarities. Differentiation; research in biological diversity. 30(1): 61-7, 1985.
  • Campbell, MA.Brown, KS.Hassell, JR.Horigan, EA.Keeler, RF. Inhibition of limb chondrogenesis by a Veratrum alkaloid: temporal specificity in vivo and in vitro. Developmental biology. 111(2): 464-70, 1985.
  • Martin, GR.Kleinman, HK.Terranova, VP.Ledbetter, S.Hassell, JR. The regulation of basement membrane formation and cell-matrix interactions by defined supramolecular complexes. Ciba Foundation symposium. 108(): 197-212, 1984.
  • Bernard, BA.De Luca, LM.Hassell, JR.Yamada, KM.Olden, K. Retinoic acid alters the proportion of high mannose to complex type oligosaccharides on fibronectin secreted by cultured chondrocytes. The Journal of biological chemistry. 259(8): 5310-5, 1984.
  • Tyree, B.Horigan, EA.Klippenstein, DL.Hassell, JR. Heterogeneity of heparan sulfate proteoglycans synthesized by PYS-2 cells. Archives of biochemistry and biophysics. 231(2): 328-35, 1984.
  • Chandrasekhar, S.Kleinman, HK.Hassell, JR.Martin, GR.Termine, JD.Trelstad, RL. Regulation of type I collagen fibril assembly by link protein and proteoglycans. Collagen and related research. 4(5): 323-37, 1984.
  • Nakazawa, K.Hassell, JR.Hascall, VC.Lohmander, LS.Newsome, DA.Krachmer, J. Defective processing of keratan sulfate in macular corneal dystrophy. The Journal of biological chemistry. 259(22): 13751-7, 1984.
  • Fisher, LW.Termine, JD.Dejter, SW.Whitson, SW.Yanagishita, M.Kimura, JH.Hascall, VC.Kleinman, HK.Hassell, JR.Nilsson, B. Proteoglycans of developing bone. The Journal of biological chemistry. 258(10): 6588-94, 1983.
  • Nakazawa, K.Newsome, DA.Nilsson, B.Hascall, VC.Hassell, JR. Purification of keratan sulfate proteoglycan from monkey cornea. The Journal of biological chemistry. 258(10): 6051-5, 1983.
  • Nakazawa, K.Hassell, JR.Hascall, VC.Newsome, DA. Heterogeneity of proteoglycans in monkey corneal stroma. Archives of biochemistry and biophysics. 222(1): 105-16, 1983.
  • Mynderse, LA.Hassell, JR.Kleinman, HK.Martin, GR.Martinez-Hernandez, A. Loss of heparan sulfate proteoglycan from glomerular basement membrane of nephrotic rats. Laboratory investigation; a journal of technical methods and pathology. 48(3): 292-302, 1983.
  • Hassell, JR.Cintron, C.Kublin, C.Newsome, DA. Proteoglycan changes during restoration of transparency in corneal scars. Archives of biochemistry and biophysics. 222(2): 362-9, 1983.
  • Woodley, DT.Rao, CN.Hassell, JR.Liotta, LA.Martin, GR.Kleinman, HK. Interactions of basement membrane components. Biochimica et biophysica acta. 761(3): 278-83, 1983.
  • Kleinman, HK.McGarvey, ML.Hassell, JR.Martin, GR. Formation of a supramolecular complex is involved in the reconstitution of basement membrane components. Biochemistry. 22(21): 4969-74, 1983.
  • Chandrasekhar, S.Kleinman, HK.Hassell, JR. Interaction of link protein with collagen. The Journal of biological chemistry. 258(10): 6226-31, 1983.
  • Nilsson, B.Nakazawa, K.Hassell, JR.Newsome, DA.Hascall, VC. Structure of oligosaccharides and the linkage region between keratan sulfate and the core protein on proteoglycans from monkey cornea. The Journal of biological chemistry. 258(10): 6056-63, 1983.
  • Newsome, DA.Harne, LC.Brown, KS.Hassell, JR. Corneal keratinization in the oel/ + mouse. Current eye research. 2(8): 553-60, 1983.
  • Poole, AR.Pidoux, I.Reiner, A.Cöster, L.Hassell, JR. Mammalian eyes and associated tissues contain molecules that are immunologically related to cartilage proteoglycan and link protein. The Journal of cell biology. 93(3): 910-20, 1982.
  • Varner, HH.Hewitt, AT.Hassell, JR.Jerdan, JA.Horigan, EA.Martin, GR. Isolation of a protein factor which suppresses the chondrocyte phenotype. Progress in clinical and biological research. 110 Pt B(): 105-11, 1982.
  • Newsome, DA.Hassell, JR.Rodrigues, MM.Rahe, AE.Krachmer, JH. Biochemical and histological analysis of "recurrent' macular corneal dystrophy. Archives of ophthalmology. 100(7): 1125-31, 1982.
  • Newsome, DA.Gross, J.Hassell, JR. Human corneal stroma contains three distinct collagens. Investigative ophthalmology & visual science. 22(3): 376-81, 1982.
  • Rohrbach, DH.Hassell, JR.Kleinman, HK.Martin, GR. Alterations in the basement membrane (heparan sulfate) proteoglycan in diabetic mice. Diabetes. 31(2): 185-8, 1982.
  • Hassell, JR.Horigan, EA. Chondrogenesis: a model developmental system for measuring teratogenic potential of compounds. Teratogenesis, carcinogenesis, and mutagenesis. 2(3-4): 325-31, 1982.
  • Newsome, DA.Foidart, JM.Hassell, JR.Krachmer, JH.Rodrigues, MM.Katz, SI. Detection of specific collagen types in normal and keratoconus corneas. Investigative ophthalmology & visual science. 20(6): 738-50, 1981.
  • Hassell, JR.Newsome, DA.Martin, GR. Isolation and characterization of the proteoglycans and collagens synthesized by cells in culture. Vision research. 21(1): 49-53, 1981.
  • Hassell, JR.Newsome, DA. Vitamin A-induced alterations in corneal and conjunctival epithelial glycoprotein biosynthesis. Annals of the New York Academy of Sciences. 359(): 358-65, 1981.
  • Harnisch, JP.Barrach, HJ.Hassell, JR.Sinha, PK. Identification of a basement membrane proteoglycan in exfoliation material. Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology. 215(4): 273-8, 1981.
  • Hassell, JR.Newsome, DA.Krachmer, JH.Rodrigues, MM. Macular corneal dystrophy: failure to synthesize a mature keratan sulfate proteoglycan. Proceedings of the National Academy of Sciences of the United States of America. 77(6): 3705-9, 1980.
  • Hassell, JR.Robey, PG.Barrach, HJ.Wilczek, J.Rennard, SI.Martin, GR. Isolation of a heparan sulfate-containing proteoglycan from basement membrane. Proceedings of the National Academy of Sciences of the United States of America. 77(8): 4494-8, 1980.
  • Hassell, JR.Newsome, DA.De Luca, LM. Increased biosynthesis of specific glycoconjugates in rat corneal epithelium following treatment with vitamin A. Investigative ophthalmology & visual science. 19(6): 642-7, 1980.
  • Hassell, JR.Newsome, DA.Hascall, VC. Characterization and biosynthesis of proteoglycans of corneal stroma from rhesus monkey. The Journal of biological chemistry. 254(24): 12346-54, 1979.
  • Hassell, JR.Pennypacker, JP.Kleinman, HK.Pratt, RM.Yamada, KM. Enhanced cellular fibronectin accumulation in chondrocytes treated with vitamin A. Cell. 17(4): 821-6, 1979.
  • Pennypacker, JP.Hassell, JR.Yamada, KM.Pratt, RM. The influence of an adhesive cell surface protein on chondrogenic expression in vitro. Experimental cell research. 121(2): 411-5, 1979.
  • Hassell, JR.Pennypacker, JP.Yamada, KM.Pratt, RM. Changes in cell surface proteins during normal and vitamin A-inhibited chondrogenesis in vitro. Annals of the New York Academy of Sciences. 312(): 406-9, 1978.
  • Lewis, CA.Pratt, RM.Pennypacker, JP.Hassell, JR. Inhibition of limb chondrogenesis in vitro by vitamin A: alterations in cell surface characteristics. Developmental biology. 64(1): 31-47, 1978.
  • Hassell, JR.Pennypacker, JP.Lewis, CA. Chondrogenesis and cell proliferation in limb bud cell cultures treated with cytosine arabinoside and vitamin A. Experimental cell research. 112(2): 409-17, 1978.
  • Hassell, JR.Silverman-Jones, CS.De Luca, LM. The in vivo stimulation of mannose incorporation into mannosylretinylphosphate, dolichylmannosylphosphate, and specific glycopeptides of rat liver by high doses of retinylpalmitate. The Journal of biological chemistry. 253(5): 1627-31, 1978.
  • Pennypacker, JP.Lewis, CA.Hassell, JR. Altered proteoglycan metabolism in mouse limb mesenchyme cell cultures treated with vitamin A. Archives of biochemistry and biophysics. 186(2): 351-8, 1978.
  • Hassell, JR.Greenberg, JH.Johnston, MC. Inhibition of cranial neural crest cell development by vitamin A in the cultured chick embryo. Journal of embryology and experimental morphology. 39(): 267-71, 1977.
  • Hassell, JR.Pratt, RM. Elevated levels of cAMP alters the effect of epidermal growth factor in vitro on programmed cell death in the secondary palatal epithelium. Experimental cell research. 106(1): 55-62, 1977.
  • Hand, AR.Hassell, JR. Tissue fixation with diimidoesters as an alternative to aldehydes. II. Cytochemical and biochemical studies of rat liver fixed with dimethylsuberimidate. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 24(9): 1000-11, 1976.
  • Hassel, JR.Orkin, RW. Synthesis and distribution of collagen in the rat palate during shelf elevation. Developmental biology. 49(1): 80-8, 1976.
  • Hassell, JR. The development of rat palatal shelves in vitro. An ultrastructural analysis of the inhibition of epithelial cell death and palate fusion by the epidermal growth factor. Developmental biology. 45(1): 90-102, 1975.
  • Pratt, RM.Hassell, JR. Appearance and distribution of carbohydrate-rich macromolecules on the epithelial surface of the developing rat palatal shelf. Developmental biology. 45(1): 192-8, 1975.
  • Johnston, MC.Hassell, JR.Brown, KS. The embryology of cleft lip and cleft palate. Clinics in plastic surgery. 2(2): 195-203, 1975.
  • Hassell, J.Klein, NW. A quantitative analysis of ovalbumin utilization by the cultured chick embryo and its relationship to growth regulation during development. Developmental biology. 26(3): 380-92, 1971.
  • Hassell, J.Yager, JD.Klein, NW. Incorporation of 14C-amino acids into egg proteins by the laying hen. Poultry science. 49(4): 1121-5, 1970.

  • Positions Held
  • Professor (Biochemistry and Molecular Biology, University of South Florida 1997 - 2006)
  • Director of Research (Research Department, Shriners Hospitals for Children 1997 - 2006)
  • Professor (Secondary Appointment) (Cell Biology and Physiology, University of Pittsburgh 1997 - 1997)
  • Professor (Ophthalmology, University of Pittsburgh 1988 - 1997)
  • Professor (Secondary Appointment) (Molecular Genetics and Biochemistry, University of Pittsburgh 1988 - 1996)
  • Research Biologist (National Institute of Dental Research, NIH 1980 - 1988)
  • Research Biologist (National Eye Institute, NIH 1978 - 1980)
  • Staff Fellow (National Institute of Dental Research, NIH 1972 - 1978)


  • Memberships
  • American Society for Biological Chemists (Member, 1987 - Present)
  • Association for Research in Vision and Ophthalmology (Member, 1979 - Present)
  • American Society for Cell Biology (Member, 1974 - Present)


  • Awards/Honors
  • Burroughs Wellcome Visiting Professor (University of North Dakota - 1999)
  • Berry Endowed Chair (University of South Florida - 1997)
  • Merit Award for R01 EY08104 (NIH - 1994)
  • Research Institute Award (Alcon - 1991)
  • Jules Stein Professorship (University of Pittsburgh - 1988)
  • The NIH Directors Award (NIH - 1985)
  • NIH Fellowship (University of Connecticut - 1970)
  • UCONN Fellowship (University of Connecticut - 1968)
  • Charles O'Hara Scholarship (Marquette University - 1968)
  • NDEA Title IV (Marquette University - 1967)
  • NIH Trainee (Marquette University - 1966)


  • Profile last modified on 03/20/2012