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John Hassell, Ph.D.
Professor, Molecular Medicine

Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

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Contacts
Email: jhassell@health.usf.edu
Phone: (813) 974-7881
Office: MDC, 3014
Mailing address University of South Florida 12901 Bruce B Downs Blvd., MDC 7
Tampa, FL 33612-4799
 
Education
  • PHD, BIOLOGY, University Of Connecticut, STORRS, United States - 1972.
  • BA, Biology, Central Connecticut State, USA - 1966.
  •  
    Interdisciplinary & Emerging Signature Programs
    Biomedical Engineering & Nanomedicine.
     
    Research Summary

    My research has focused on identifying the proteoglycans present in cartilage, basement membrane and cornea and determining their role in disease and development. My current research interest is in the extra cellular matrix (ECM) made by keratocytes during wound healing. The corneal stroma consists of an extensive extra cellular ECM interspersed with keratocytes. This ECM is uniquely transparent due to the small uniform diameter and spacing of the collagen fibrils. Wounds to the cornea readily heal but the stomal keratocytes can often produce opaque scars that cause blindness. Stromal wound healing is currently thought to consist of three major phases. First, the injured keratocytes as well as some keratocytes flanking the wound undergo apoptosis. Second, some of the surviving keratocytes are activated to proliferate and become hypercellular myofibroblasts (myofibroblasts that produce little ECM, resulting in densely packed cells). The hypercellular myofibroblasts can remain as such and produce a hypercellular scar, or they can be stimulated to produce one of two kinds of ECM: 1) a normal collagenous matrix that restores transparency, 2) a fibrocollagenous matrix that produces an opaque scar. We have found, by using keratocytes in culture that the hypercellular and collagenous phases of wound healing can be reproduced in vitro by specific growth factors. FGF-2 and TGF-beta together would produce the hypercellular phase, IGF-1 alone would produce the collagenous phase and TGF-beta alone would produce the fibrocollagenous phase. We are now using these growth factors in tissue engineering studies to produce a stromal construct for use in corneal transplantation.

     


    Selected Publications
  • Etheredge L, Kane B, Hassell JR. The effect of growth factor signaling on keratocytes in vitro and its relationship to the phases of stromal wound repair. Investigative ophthalmology & visual science. : , 2009.
  • Kane BP, Jester JV, Huang J, Wahlert A, Hassell JR. IGF-II and collagen expression by keratocytes during postnatal development. Experimental eye research. : , 2009.
  • Hassell JR, Kane BP, Etheredge LT, Valkov N, Birk DE. Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose. Experimental eye research. 87(6) : 604-11, 2008.
  • Musselmann K, Kane BP, Alexandrou B, Hassell JR. IGF-II is present in bovine corneal stroma and activates keratocytes to proliferate in vitro. Experimental eye research. 86(3) : 506-11, 2008.
  • Smith SM, West LA, Govindraj P, Zhang X, Ornitz DM, Hassell JR. Heparan and chondroitin sulfate on growth plate perlecan mediate binding and delivery of FGF-2 to FGF receptors. Matrix biology : journal of the International Society for Matrix Biology. 26(3) : 175-84, 2007.
  • Smith SM, West LA, Hassell JR. The core protein of growth plate perlecan binds FGF-18 and alters its mitogenic effect on chondrocytes. Archives of biochemistry and biophysics. 468(2) : 244-51, 2007.
  • West L, Govindraj P, Koob TJ, Hassell JR. Changes in perlecan during chondrocyte differentiation in the fetal bovine rib growth plate. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 24(6) : 1317-26, 2006.
  • Govindraj P, West L, Smith S, Hassell JR. Modulation of FGF-2 binding to chondrocytes from the developing growth plate by perlecan. Matrix biology : journal of the International Society for Matrix Biology. 25(4) : 232-9, 2006.
  • Musselmann K, Kane B, Alexandrou B, Hassell JR. Stimulation of collagen synthesis by insulin and proteoglycan accumulation by ascorbate in bovine keratocytes in vitro. Investigative ophthalmology & visual science. 47(12) : 5260-6, 2006.
  • Musselmann K, Hassell JR. Focus on molecules: CHST6 (carbohydrate sulfotransferase 6; corneal N-acetylglucosamine-6-sulfotransferase). Experimental eye research. 83(4) : 707-8, 2006.
  • Smith S, Hassell JR. Focus on molecules: perlecan (HSPG2). Experimental eye research. 83(3) : 471-2, 2006.
  • Musselmann K, Alexandrou B, Kane B, Hassell JR. Maintenance of the keratocyte phenotype during cell proliferation stimulated by insulin. The Journal of biological chemistry. 280(38) : 32634-9, 2005.
  • Fukuchi K, Hart M, Yan Z, Hassell JR, Li L. Transgenic mice overexpressing both amyloid beta-protein and perlecan in pancreatic acinar cells. Histology and histopathology. 19(3) : 845-52, 2004.
  • Musselmann K, Kane BP, Hassell JR. Isolation of a putative keratocyte activating factor from the corneal stroma. Experimental eye research. 77(3) : 273-9, 2003.
  • Liu CY, Birk DE, Hassell JR, Kane B, Kao WW. Keratocan-deficient mice display alterations in corneal structure. The Journal of biological chemistry. 278(24) : 21672-7, 2003.
  • Berryhill BL, Kane B, Stramer BM, Fini ME, Hassell JR. Increased SPARC accumulation during corneal repair. Experimental eye research. 77(1) : 85-92, 2003.
  • Govindraj P, West L, Koob TJ, Neame P, Doege K, Hassell JR. Isolation and identification of the major heparan sulfate proteoglycans in the developing bovine rib growth plate. The Journal of biological chemistry. 277(22) : 19461-9, 2002.
  • Arikawa-Hirasawa E, Le AH, Nishino I, Nonaka I, Ho NC, Francomano CA, Govindraj P, Hassell JR, Devaney JM, Spranger J, Stevenson RE, Iannaccone S, Dalakas MC, Yamada Y. Structural and functional mutations of the perlecan gene cause Schwartz-Jampel syndrome, with myotonic myopathy and chondrodysplasia. American journal of human genetics. 70(5) : 1368-75, 2002.
  • Berryhill BL, Kader R, Kane B, Birk DE, Feng J, Hassell JR. Partial restoration of the keratocyte phenotype to bovine keratocytes made fibroblastic by serum. Investigative ophthalmology & visual science. 43(11) : 3416-21, 2002.
  • Pandit SG, Govindraj P, Sasse J, Neame PJ, Hassell JR. The fibroblast growth factor receptor, FGFR3, forms gradients of intact and degraded protein across the growth plate of developing bovine ribs. The Biochemical journal. 361(Pt 2) : 231-41, 2002.
  • Berryhill BL, Beales MP, Hassell JR. Production of prostaglandin D synthase as a keratan sulfate proteoglycan by cultured bovine keratocytes. Investigative ophthalmology & visual science. 42(6) : 1201-7, 2001.
  • Hart M, Li L, Tokunaga T, Lindsey JR, Hassell JR, Snow AD, Fukuchi K. Overproduction of perlecan core protein in cultured cells and transgenic mice. The Journal of pathology. 194(2) : 262-9, 2001.
  • Arikawa-Hirasawa E, Wilcox WR, Le AH, Silverman N, Govindraj P, Hassell JR, Yamada Y. Dyssegmental dysplasia, Silverman-Handmaker type, is caused by functional null mutations of the perlecan gene. Nature genetics. 27(4) : 431-4, 2001.
  • Dunlevy JR, Beales MP, Berryhill BL, Cornuet PK, Hassell JR. Expression of the keratan sulfate proteoglycans lumican, keratocan and osteoglycin/mimecan during chick corneal development. Experimental eye research. 70(3) : 349-62, 2000.
  • Neame PJ, Kay CJ, McQuillan DJ, Beales MP, Hassell JR. Independent modulation of collagen fibrillogenesis by decorin and lumican. Cellular and molecular life sciences : CMLS. 57(5) : 859-63, 2000.
  • Chakravarti S, Petroll WM, Hassell JR, Jester JV, Lass JH, Paul J, Birk DE. Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma. Investigative ophthalmology & visual science. 41(11) : 3365-73, 2000.
  • Corpuz LM, Dunlevy JR, Hassell JR, Conrad AH, Conrad GW. Molecular cloning and relative tissue expression of decorin and lumican in embryonic quail cornea. Matrix biology : journal of the International Society for Matrix Biology. 19(7) : 699-704, 2000.
  • Corpuz LM, Dunlevy JR, Hassell JR, Conrad AH, Conrad GW. Molecular cloning and relative tissue expression of keratocan and mimecan in embryonic quail cornea. Matrix biology : journal of the International Society for Matrix Biology. 19(7) : 693-8, 2000.
  • Beales MP, Funderburgh JL, Jester JV, Hassell JR. Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of the keratocyte phenotype in culture. Investigative ophthalmology & visual science. 40(8) : 1658-63, 1999.
  • Dunlevy JR, Berryhill BL, Vergnes JP, SundarRaj N, Hassell JR. Cloning, chromosomal localization, and characterization of cDNA from a novel gene, SH3BP4, expressed by human corneal fibroblasts. Genomics. 62(3) : 519-24, 1999.
  • Tapanadechopone P, Hassell JR, Rigatti B, Couchman JR. Localization of glycosaminoglycan substitution sites on domain V of mouse perlecan. Biochemical and biophysical research communications. 265(3) : 680-90, 1999.
  • Arikawa-Hirasawa E, Watanabe H, Takami H, Hassell JR, Yamada Y. Perlecan is essential for cartilage and cephalic development. Nature genetics. 23(3) : 354-8, 1999.
  • Villar MJ, Hassell JR, Brandan E. Interaction of skeletal muscle cells with collagen type IV is mediated by perlecan associated with the cell surface. Journal of cellular biochemistry. 75(4) : 665-74, 1999.
  • Sundarraj N, Fite D, Belak R, Sundarraj S, Rada J, Okamoto S, Hassell J. Proteoglycan distribution during healing of corneal stromal wounds in chick. Experimental eye research. 67(4) : 433-42, 1998.
  • Dunlevy JR, Chakravarti S, Gyalzen P, Vergnes JP, Hassell JR. Cloning and chromosomal localization of mouse keratocan, a corneal keratan sulfate proteoglycan. Mammalian genome : official journal of the International Mammalian Genome Society. 9(4) : 316-9, 1998.
  • Hassell JR, Rada J, Cornuet P, Vergnes JP, Kinchington PR. Gene structure of chick lumican and identification of the first exon. Biochimica et biophysica acta. 1397(2) : 119-25, 1998.
  • Peng HB, Ali AA, Daggett DF, Rauvala H, Hassell JR, Smalheiser NR. The relationship between perlecan and dystroglycan and its implication in the formation of the neuromuscular junction. Cell adhesion and communication. 5(6) : 475-89, 1998.
  • Dunlevy JR, Neame PJ, Vergnes JP, Hassell JR. Identification of the N-linked oligosaccharide sites in chick corneal lumican and keratocan that receive keratan sulfate. The Journal of biological chemistry. 273(16) : 9615-21, 1998.
  • Hassell JR, Klintworth GK. Serum sulfotransferase levels in patients with macular corneal dystrophy type I. Archives of ophthalmology. 115(11) : 1419-21, 1997.
  • Larraín J, Alvarez J, Hassell JR, Brandan E. Expression of perlecan, a proteoglycan that binds myogenic inhibitory basic fibroblast growth factor, is down regulated during skeletal muscle differentiation. Experimental cell research. 234(2) : 405-12, 1997.
  • Dolan M, Horchar T, Rigatti B, Hassell JR. Identification of sites in domain I of perlecan that regulate heparan sulfate synthesis. The Journal of biological chemistry. 272(7) : 4316-22, 1997.
  • Rada JA, Fini ME, Hassell JR. Regionalized growth patterns of young chicken corneas. Investigative ophthalmology & visual science. 37(10) : 2060-7, 1996.
  • Couchman JR, Ljubimov AV, Sthanam M, Horchar T, Hassell JR. Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 43(9) : 955-63, 1995.
  • SundarRaj N, Fite D, Ledbetter S, Chakravarti S, Hassell JR. Perlecan is a component of cartilage matrix and promotes chondrocyte attachment. Journal of cell science. 108 ( Pt 7): 2663-72, 1995.
  • Chakravarti S, Stallings RL, SundarRaj N, Cornuet PK, Hassell JR. Primary structure of human lumican (keratan sulfate proteoglycan) and localization of the gene (LUM) to chromosome 12q21.3-q22. Genomics. 27(3) : 481-8, 1995.
  • Chakravarti S, Horchar T, Jefferson B, Laurie GW, Hassell JR. Recombinant domain III of perlecan promotes cell attachment through its RGDS sequence. The Journal of biological chemistry. 270(1) : 404-9, 1995.
  • Okamoto S, Ohji M, Kosaku K, Sundarraj N, Hassell JR, Thoft RA, Pipas JM. Characterization of immortalized rabbit corneal epithelial cells with SV40 large T antigen. Japanese journal of ophthalmology. 39(4) : 323-33, 1995.
  • Mandarino LJ, Finlayson J, Hassell JR. High glucose downregulates glucose transport activity in retinal capillary pericytes but not endothelial cells. Investigative ophthalmology & visual science. 35(3) : 964-72, 1994.
  • Ohji M, SundarRaj N, Hassell JR, Thoft RA. Basement membrane synthesis by human corneal epithelial cells in vitro. Investigative ophthalmology & visual science. 35(2) : 479-85, 1994.
  • Cornuet PK, Blochberger TC, Hassell JR. Molecular polymorphism of lumican during corneal development. Investigative ophthalmology & visual science. 35(3) : 870-7, 1994.
  • Funderburgh JL, Funderburgh ML, Brown SJ, Vergnes JP, Hassell JR, Mann MM, Conrad GW. Sequence and structural implications of a bovine corneal keratan sulfate proteoglycan core protein. Protein 37B represents bovine lumican and proteins 37A and 25 are unique. The Journal of biological chemistry. 268(16) : 11874-80, 1993.
  • Rada JA, Cornuet PK, Hassell JR. Regulation of corneal collagen fibrillogenesis in vitro by corneal proteoglycan (lumican and decorin) core proteins. Experimental eye research. 56(6) : 635-48, 1993.
  • Noonan DM, Hassell JR. Perlecan, the large low-density proteoglycan of basement membranes: structure and variant forms. Kidney international. 43(1) : 53-60, 1993.
  • Chakravarti S, Hassell JR, Phillips SL. Perlecan gene expression precedes laminin gene expression during differentiation of F9 embryonal carcinoma cells. Developmental dynamics : an official publication of the American Association of Anatomists. 197(2) : 107-14, 1993.
  • Rescan PY, Loréal O, Hassell JR, Yamada Y, Guillouzo A, Clément B. Distribution and origin of the basement membrane component perlecan in rat liver and primary hepatocyte culture. The American journal of pathology. 142(1) : 199-208, 1993.
  • Blochberger TC, Cornuet PK, Hassell JR. Isolation and partial characterization of lumican and decorin from adult chicken corneas. A keratan sulfate-containing isoform of decorin is developmentally regulated. The Journal of biological chemistry. 267(29) : 20613-9, 1992.
  • Schrecengost PK, Blochberger TC, Hassell JR. Identification of chick corneal keratan sulfate proteoglycan precursor protein in whole corneas and in cultured corneal fibroblasts. Archives of biochemistry and biophysics. 292(1) : 54-61, 1992.
  • Blochberger TC, Vergnes JP, Hempel J, Hassell JR. cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine. The Journal of biological chemistry. 267(1) : 347-52, 1992.
  • Danielson KG, Martinez-Hernandez A, Hassell JR, Iozzo RV. Establishment of a cell line from the EHS tumor: biosynthesis of basement membrane constituents and characterization of a hybrid proteoglycan containing heparan and chondroitin sulfate chains. Matrix (Stuttgart, Germany). 12(1) : 22-35, 1992.
  • Hassell JR, Schrecengost PK, Rada JA, SundarRaj N, Sossi G, Thoft RA. Biosynthesis of stromal matrix proteoglycans and basement membrane components by human corneal fibroblasts. Investigative ophthalmology & visual science. 33(3) : 547-57, 1992.
  • Li W, Vergnes JP, Cornuet PK, Hassell JR. cDNA clone to chick corneal chondroitin/dermatan sulfate proteoglycan reveals identity to decorin. Archives of biochemistry and biophysics. 296(1) : 190-7, 1992.
  • Rada JA, McFarland AL, Cornuet PK, Hassell JR. Proteoglycan synthesis by scleral chondrocytes is modulated by a vision dependent mechanism. Current eye research. 11(8) : 767-82, 1992.
  • Noonan DM, Fulle A, Valente P, Cai S, Horigan E, Sasaki M, Yamada Y, Hassell JR. The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule. The Journal of biological chemistry. 266(34) : 22939-47, 1991.
  • Chakravarti S, Phillips SL, Hassell JR. Assignment of the perlecan (heparan sulfate proteoglycan) gene to mouse chromosome 4. Mammalian genome : official journal of the International Mammalian Genome Society. 1(4) : 270-2, 1991.
  • Dodge GR, Kovalszky I, Chu ML, Hassell JR, McBride OW, Yi HF, Iozzo RV. Heparan sulfate proteoglycan of human colon: partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1. Genomics. 10(3) : 673-80, 1991.
  • Jost CJ, Funderburgh JL, Mann M, Hassell JR, Conrad GW. Cell-free translation and characterization of corneal keratan sulfate proteoglycan core proteins. The Journal of biological chemistry. 266(20) : 13336-41, 1991.
  • Rada JA, Thoft RA, Hassell JR. Increased aggrecan (cartilage proteoglycan) production in the sclera of myopic chicks. Developmental biology. 147(2) : 303-12, 1991.
  • Midura RJ, Hascall VC, MacCallum DK, Meyer RF, Thonar EJ, Hassell JR, Smith CF, Klintworth GK. Proteoglycan biosynthesis by human corneas from patients with types 1 and 2 macular corneal dystrophy. The Journal of biological chemistry. 265(26) : 15947-55, 1990.
  • Dodge GR, Kovalszky I, Hassell JR, Iozzo RV. Transforming growth factor beta alters the expression of heparan sulfate proteoglycan in human colon carcinoma cells. The Journal of biological chemistry. 265(29) : 18023-9, 1990.
  • Ledbetter S, Copeland EJ, Noonan D, Vogeli G, Hassell JR. Altered steady-state mRNA levels of basement membrane proteins in diabetic mouse kidneys and thromboxane synthase inhibition. Diabetes. 39(2) : 196-203, 1990.
  • Iozzo RV, Hassell JR. Identification of the precursor protein for the heparan sulfate proteoglycan of human colon carcinoma cells and its post-translational modifications. Archives of biochemistry and biophysics. 269(1) : 239-49, 1989.
  • Grant DS, Leblond CP, Kleinman HK, Inoue S, Hassell JR. The incubation of laminin, collagen IV, and heparan sulfate proteoglycan at 35 degrees C yields basement membrane-like structures. The Journal of cell biology. 108(4) : 1567-74, 1989.
  • Clément B, Segui-Real B, Hassell JR, Martin GR, Yamada Y. Identification of a cell surface-binding protein for the core protein of the basement membrane proteoglycan. The Journal of biological chemistry. 264(21) : 12467-71, 1989.
  • Mukhopadhyay NK, Shome K, Saha AK, Hassell JR, Glew RH. Heparin binds to Leishmania donovani promastigotes and inhibits protein phosphorylation. The Biochemical journal. 264(2) : 517-25, 1989.
  • Klein DJ, Brown DM, Oegema TR, Brenchley PE, Anderson JC, Dickinson MA, Horigan EA, Hassell JR. Glomerular basement membrane proteoglycans are derived from a large precursor. The Journal of cell biology. 106(3) : 963-70, 1988.
  • Newsome DA, Pfeffer BA, Hewitt AT, Robey PG, Hassell JR. Detection of extracellular matrix molecules synthesized in vitro by monkey and human retinal pigment epithelium: influence of donor age and multiple passages. Experimental eye research. 46(3) : 305-21, 1988.
  • Horton WE, Cleveland J, Rapp U, Nemuth G, Bolander M, Doege K, Yamada Y, Hassell JR. An established rat cell line expressing chondrocyte properties. Experimental cell research. 178(2) : 457-68, 1988.
  • Laurie GW, Inoue S, Bing JT, Hassell JR. Visualization of the large heparan sulfate proteoglycan from basement membrane. The American journal of anatomy. 181(3) : 320-6, 1988.
  • Noonan DM, Horigan EA, Ledbetter SR, Vogeli G, Sasaki M, Yamada Y, Hassell JR. Identification of cDNA clones encoding different domains of the basement membrane heparan sulfate proteoglycan. The Journal of biological chemistry. 263(31) : 16379-87, 1988.
  • Singer II, Scott S, Kawka DW, Hassell JR. Extracellular matrix fibers containing fibronectin and basement membrane heparan sulfate proteoglycan coalign with focal contacts and microfilament bundles in stationary fibroblasts. Experimental cell research. 173(2) : 558-71, 1987.
  • Horton W, Miyashita T, Kohno K, Hassell JR, Yamada Y. Identification of a phenotype-specific enhancer in the first intron of the rat collagen II gene. Proceedings of the National Academy of Sciences of the United States of America. 84(24) : 8864-8, 1987.
  • Doege K, Sasaki M, Horigan E, Hassell JR, Yamada Y. Complete primary structure of the rat cartilage proteoglycan core protein deduced from cDNA clones. The Journal of biological chemistry. 262(36) : 17757-67, 1987.
  • Newsome DA, Hewitt AT, Huh W, Robey PG, Hassell JR. Detection of specific extracellular matrix molecules in drusen, Bruch's membrane, and ciliary body. American journal of ophthalmology. 104(4) : 373-81, 1987.
  • Ledbetter SR, Fisher LW, Hassell JR. Domain structure of the basement membrane heparan sulfate proteoglycan. Biochemistry. 26(4) : 988-95, 1987.
  • Ledbetter SR, Wagner CW, Martin GR, Rohrbach DH, Hassell JR. Response of diabetic basement membrane--producing cells to glucose and insulin. Diabetes. 36(9) : 1029-34, 1987.
  • Horton WE, Yamada Y, Hassell JR. Retinoic acid rapidly reduces cartilage matrix synthesis by altering gene transcription in chondrocytes. Developmental biology. 123(2) : 508-16, 1987.
  • Klintworth GK, Meyer R, Dennis R, Hewitt AT, Stock EL, Lenz ME, Hassell JR, Stark WJ, Kuettner KE, Thonar EJ. Macular corneal dystrophy. Lack of keratan sulfate in serum and cornea. Ophthalmic paediatrics and genetics. 7(3) : 139-43, 1986.
  • Thonar EJ, Meyer RF, Dennis RF, Lenz ME, Maldonado B, Hassell JR, Hewitt AT, Stark WJ, Stock EL, Kuettner KE. Absence of normal keratan sulfate in the blood of patients with macular corneal dystrophy. American journal of ophthalmology. 102(5) : 561-9, 1986.
  • Tyree B, Hassell JR, Hascall VC. Altered synthesis of heparan sulfate proteoglycans at low sulfate concentration. Archives of biochemistry and biophysics. 250(1) : 202-10, 1986.
  • Doege K, Fernandez P, Hassell JR, Sasaki M, Yamada Y. Partial cDNA sequence encoding a globular domain at the C terminus of the rat cartilage proteoglycan. The Journal of biological chemistry. 261(18) : 8108-11, 1986.
  • Hassell JR, Kimura JH, Hascall VC. Proteoglycan core protein families. Annual review of biochemistry. 55: 539-67, 1986.
  • Doege K, Hassell JR, Caterson B, Yamada Y. Link protein cDNA sequence reveals a tandemly repeated protein structure. Proceedings of the National Academy of Sciences of the United States of America. 83(11) : 3761-5, 1986.
  • Kleinman HK, McGarvey ML, Hassell JR, Star VL, Cannon FB, Laurie GW, Martin GR. Basement membrane complexes with biological activity. Biochemistry. 25(2) : 312-8, 1986.
  • Ledbetter SR, Hassell JR. beta-D-xyloside-mediated alteration in the synthesis of basement membrane proteoglycan. Archives of biochemistry and biophysics. 246(1) : 403-10, 1986.
  • Laurie GW, Bing JT, Kleinman HK, Hassell JR, Aumailley M, Martin GR, Feldmann RJ. Localization of binding sites for laminin, heparan sulfate proteoglycan and fibronectin on basement membrane (type IV) collagen. Journal of molecular biology. 189(1) : 205-16, 1986.
  • Horton W, Hassell JR. Independence of cell shape and loss of cartilage matrix production during retinoic acid treatment of cultured chondrocytes. Developmental biology. 115(2) : 392-7, 1986.
  • Hassell JR, Leyshon WC, Ledbetter SR, Tyree B, Suzuki S, Kato M, Kimata K, Kleinman HK. Isolation of two forms of basement membrane proteoglycans. The Journal of biological chemistry. 260(13) : 8098-105, 1985.
  • Ledbetter SR, Tyree B, Hassell JR, Horigan EA. Identification of the precursor protein to basement membrane heparan sulfate proteoglycans. The Journal of biological chemistry. 260(13) : 8106-113, 1985.
  • Campbell MA, Brown KS, Hassell JR, Horigan EA, Keeler RF. Inhibition of limb chondrogenesis by a Veratrum alkaloid: temporal specificity in vivo and in vitro. Developmental biology. 111(2) : 464-70, 1985.
  • Hassell JR, Horigan EA, Mosley GL, Ledbetter SR, Kleinman HK, Chandrasekhar S. Appearance of basement membrane components during the differentiation of limb bud mesenchyme cells in culture. Progress in clinical and biological research. 171: 75-86, 1985.
  • Wewer UM, Albrechtsen R, Hassell JR. Heparan sulfate proteoglycans made by different basement-membrane-producing tumors have immunological and structural similarities. Differentiation; research in biological diversity. 30(1) : 61-7, 1985.
  • Kleinman HK, Cannon FB, Laurie GW, Hassell JR, Aumailley M, Terranova VP, Martin GR, DuBois-Dalcq M. Biological activities of laminin. Journal of cellular biochemistry. 27(4) : 317-25, 1985.
  • Tyree B, Horigan EA, Klippenstein DL, Hassell JR. Heterogeneity of heparan sulfate proteoglycans synthesized by PYS-2 cells. Archives of biochemistry and biophysics. 231(2) : 328-35, 1984.
  • Chandrasekhar S, Kleinman HK, Hassell JR, Martin GR, Termine JD, Trelstad RL. Regulation of type I collagen fibril assembly by link protein and proteoglycans. Collagen and related research. 4(5) : 323-37, 1984.
  • Nakazawa K, Hassell JR, Hascall VC, Lohmander LS, Newsome DA, Krachmer J. Defective processing of keratan sulfate in macular corneal dystrophy. The Journal of biological chemistry. 259(22) : 13751-7, 1984.
  • Martin GR, Kleinman HK, Terranova VP, Ledbetter S, Hassell JR. The regulation of basement membrane formation and cell-matrix interactions by defined supramolecular complexes. Ciba Foundation symposium. 108: 197-212, 1984.
  • Bernard BA, De Luca LM, Hassell JR, Yamada KM, Olden K. Retinoic acid alters the proportion of high mannose to complex type oligosaccharides on fibronectin secreted by cultured chondrocytes. The Journal of biological chemistry. 259(8) : 5310-5, 1984.
  • Fisher LW, Termine JD, Dejter SW, Whitson SW, Yanagishita M, Kimura JH, Hascall VC, Kleinman HK, Hassell JR, Nilsson B. Proteoglycans of developing bone. The Journal of biological chemistry. 258(10) : 6588-94, 1983.
  • Hassell JR, Cintron C, Kublin C, Newsome DA. Proteoglycan changes during restoration of transparency in corneal scars. Archives of biochemistry and biophysics. 222(2) : 362-9, 1983.
  • Nakazawa K, Hassell JR, Hascall VC, Newsome DA. Heterogeneity of proteoglycans in monkey corneal stroma. Archives of biochemistry and biophysics. 222(1) : 105-16, 1983.
  • Nakazawa K, Newsome DA, Nilsson B, Hascall VC, Hassell JR. Purification of keratan sulfate proteoglycan from monkey cornea. The Journal of biological chemistry. 258(10) : 6051-5, 1983.
  • Nilsson B, Nakazawa K, Hassell JR, Newsome DA, Hascall VC. Structure of oligosaccharides and the linkage region between keratan sulfate and the core protein on proteoglycans from monkey cornea. The Journal of biological chemistry. 258(10) : 6056-63, 1983.
  • Chandrasekhar S, Kleinman HK, Hassell JR. Interaction of link protein with collagen. The Journal of biological chemistry. 258(10) : 6226-31, 1983.
  • Kleinman HK, McGarvey ML, Hassell JR, Martin GR. Formation of a supramolecular complex is involved in the reconstitution of basement membrane components. Biochemistry. 22(21) : 4969-74, 1983.
  • Woodley DT, Rao CN, Hassell JR, Liotta LA, Martin GR, Kleinman HK. Interactions of basement membrane components. Biochimica et biophysica acta. 761(3) : 278-83, 1983.
  • Mynderse LA, Hassell JR, Kleinman HK, Martin GR, Martinez-Hernandez A. Loss of heparan sulfate proteoglycan from glomerular basement membrane of nephrotic rats. Laboratory investigation; a journal of technical methods and pathology. 48(3) : 292-302, 1983.
  • Newsome DA, Harne LC, Brown KS, Hassell JR. Corneal keratinization in the oel/ + mouse. Current eye research. 2(8) : 553-60, 1983.
  • Poole AR, Pidoux I, Reiner A, Cöster L, Hassell JR. Mammalian eyes and associated tissues contain molecules that are immunologically related to cartilage proteoglycan and link protein. The Journal of cell biology. 93(3) : 910-20, 1982.
  • Newsome DA, Gross J, Hassell JR. Human corneal stroma contains three distinct collagens. Investigative ophthalmology & visual science. 22(3) : 376-81, 1982.
  • Rohrbach DH, Hassell JR, Kleinman HK, Martin GR. Alterations in the basement membrane (heparan sulfate) proteoglycan in diabetic mice. Diabetes. 31(2) : 185-8, 1982.
  • Newsome DA, Hassell JR, Rodrigues MM, Rahe AE, Krachmer JH. Biochemical and histological analysis of "recurrent' macular corneal dystrophy. Archives of ophthalmology. 100(7) : 1125-31, 1982.
  • Varner HH, Hewitt AT, Hassell JR, Jerdan JA, Horigan EA, Martin GR. Isolation of a protein factor which suppresses the chondrocyte phenotype. Progress in clinical and biological research. 110 Pt B: 105-11, 1982.
  • Hassell JR, Horigan EA. Chondrogenesis: a model developmental system for measuring teratogenic potential of compounds. Teratogenesis, carcinogenesis, and mutagenesis. 2(3-4) : 325-31, 1982.
  • Newsome DA, Foidart JM, Hassell JR, Krachmer JH, Rodrigues MM, Katz SI. Detection of specific collagen types in normal and keratoconus corneas. Investigative ophthalmology & visual science. 20(6) : 738-50, 1981.
  • Harnisch JP, Barrach HJ, Hassell JR, Sinha PK. Identification of a basement membrane proteoglycan in exfoliation material. Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology. 215(4) : 273-8, 1981.
  • Hassell JR, Newsome DA, Martin GR. Isolation and characterization of the proteoglycans and collagens synthesized by cells in culture. Vision research. 21(1) : 49-53, 1981.
  • Hassell JR, Newsome DA. Vitamin A-induced alterations in corneal and conjunctival epithelial glycoprotein biosynthesis. Annals of the New York Academy of Sciences. 359: 358-65, 1981.
  • Hassell JR, Newsome DA, De Luca LM. Increased biosynthesis of specific glycoconjugates in rat corneal epithelium following treatment with vitamin A. Investigative ophthalmology & visual science. 19(6) : 642-7, 1980.
  • Hassell JR, Robey PG, Barrach HJ, Wilczek J, Rennard SI, Martin GR. Isolation of a heparan sulfate-containing proteoglycan from basement membrane. Proceedings of the National Academy of Sciences of the United States of America. 77(8) : 4494-8, 1980.
  • Hassell JR, Newsome DA, Krachmer JH, Rodrigues MM. Macular corneal dystrophy: failure to synthesize a mature keratan sulfate proteoglycan. Proceedings of the National Academy of Sciences of the United States of America. 77(6) : 3705-9, 1980.
  • Hassell JR, Newsome DA, Hascall VC. Characterization and biosynthesis of proteoglycans of corneal stroma from rhesus monkey. The Journal of biological chemistry. 254(24) : 12346-54, 1979.
  • Hassell JR, Pennypacker JP, Kleinman HK, Pratt RM, Yamada KM. Enhanced cellular fibronectin accumulation in chondrocytes treated with vitamin A. Cell. 17(4) : 821-6, 1979.
  • Pennypacker JP, Hassell JR, Yamada KM, Pratt RM. The influence of an adhesive cell surface protein on chondrogenic expression in vitro. Experimental cell research. 121(2) : 411-5, 1979.
  • Hassell JR, Pennypacker JP, Yamada KM, Pratt RM. Changes in cell surface proteins during normal and vitamin A-inhibited chondrogenesis in vitro. Annals of the New York Academy of Sciences. 312: 406-9, 1978.
  • Lewis CA, Pratt RM, Pennypacker JP, Hassell JR. Inhibition of limb chondrogenesis in vitro by vitamin A: alterations in cell surface characteristics. Developmental biology. 64(1) : 31-47, 1978.
  • Hassell JR, Pennypacker JP, Lewis CA. Chondrogenesis and cell proliferation in limb bud cell cultures treated with cytosine arabinoside and vitamin A. Experimental cell research. 112(2) : 409-17, 1978.
  • Hassell JR, Silverman-Jones CS, De Luca LM. The in vivo stimulation of mannose incorporation into mannosylretinylphosphate, dolichylmannosylphosphate, and specific glycopeptides of rat liver by high doses of retinylpalmitate. The Journal of biological chemistry. 253(5) : 1627-31, 1978.
  • Pennypacker JP, Lewis CA, Hassell JR. Altered proteoglycan metabolism in mouse limb mesenchyme cell cultures treated with vitamin A. Archives of biochemistry and biophysics. 186(2) : 351-8, 1978.
  • Hassell JR, Greenberg JH, Johnston MC. Inhibition of cranial neural crest cell development by vitamin A in the cultured chick embryo. Journal of embryology and experimental morphology. 39: 267-71, 1977.
  • Hassell JR, Pratt RM. Elevated levels of cAMP alters the effect of epidermal growth factor in vitro on programmed cell death in the secondary palatal epithelium. Experimental cell research. 106(1) : 55-62, 1977.
  • Hand AR, Hassell JR. Tissue fixation with diimidoesters as an alternative to aldehydes. II. Cytochemical and biochemical studies of rat liver fixed with dimethylsuberimidate. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 24(9) : 1000-11, 1976.
  • Hassel JR, Orkin RW. Synthesis and distribution of collagen in the rat palate during shelf elevation. Developmental biology. 49(1) : 80-8, 1976.
  • Hassell JR. The development of rat palatal shelves in vitro. An ultrastructural analysis of the inhibition of epithelial cell death and palate fusion by the epidermal growth factor. Developmental biology. 45(1) : 90-102, 1975.
  • Pratt RM, Hassell JR. Appearance and distribution of carbohydrate-rich macromolecules on the epithelial surface of the developing rat palatal shelf. Developmental biology. 45(1) : 192-8, 1975.
  • Johnston MC, Hassell JR, Brown KS. The embryology of cleft lip and cleft palate. Clinics in plastic surgery. 2(2) : 195-203, 1975.
  • Hassell J, Klein NW. A quantitative analysis of ovalbumin utilization by the cultured chick embryo and its relationship to growth regulation during development. Developmental biology. 26(3) : 380-92, 1971.
  • Hassell J, Yager JD, Klein NW. Incorporation of 14C-amino acids into egg proteins by the laying hen. Poultry science. 49(4) : 1121-5, 1970.

  • Positions Held
  • Professor (Biochemistry and Molecular Biology, University of South Florida 1997 - 2006)
  • Director of Research (Research Department, Shriners Hospitals for Children 1997 - 2006)
  • Professor (Secondary Appointment) (Cell Biology and Physiology, University of Pittsburgh 1997 - 1997)
  • Professor (Ophthalmology, University of Pittsburgh 1988 - 1997)
  • Professor (Secondary Appointment) (Molecular Genetics and Biochemistry, University of Pittsburgh 1988 - 1996)
  • Research Biologist (National Institute of Dental Research, NIH 1980 - 1988)
  • Research Biologist (National Eye Institute, NIH 1978 - 1980)
  • Staff Fellow (National Institute of Dental Research, NIH 1972 - 1978)


  • Memberships
  • American Society for Biological Chemists (Member, 1987 - Present)
  • Association for Research in Vision and Ophthalmology (Member, 1979 - Present)
  • American Society for Cell Biology (Member, 1974 - Present)


  • Awards/Honors
  • Burroughs Wellcome Visiting Professor (University of North Dakota - 1999)
  • Berry Endowed Chair (University of South Florida - 1997)
  • Merit Award for R01 EY08104 (NIH - 1994)
  • Research Institute Award (Alcon - 1991)
  • Jules Stein Professorship (University of Pittsburgh - 1988)
  • The NIH Directors Award (NIH - 1985)
  • NIH Fellowship (University of Connecticut - 1970)
  • UCONN Fellowship (University of Connecticut - 1968)
  • Charles O'Hara Scholarship (Marquette University - 1968)
  • NDEA Title IV (Marquette University - 1967)
  • NIH Trainee (Marquette University - 1966)


  • Profile last modified on 03/20/2012