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Duane Eichler, Ph.D.


Interim Chair, College of Medicine-Dept. of Molecular Medicine

Basic Science Director, Cardiovascular Signature Research Program

Prevention Research Fellow, Florida Prevention Research Center (FPRC)

Unit Director, Clinical Research and Health Management

Co-Director, Training and Professional Education Unit

Professor (Joint Appointment), Department of Pediatrics, College of Medicine

Professor, Department of Biochemistry and Molecular Biology, College of Medicine

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Email: deichler@health.usf.edu
Phone: (813) 974-9716
Office: MDC, 3537
Mailing address 12901 Bruce B. Downs Blvd. MDC 7
Tampa, FL 33612
View My C.V.
  • PHD, Biological Chemistry, University Of California At Los Angeles, Los Angeles, United States - 1973.
  • B.A., Chemistry (Conc in BIochemistry), San Jose State University, USA - 1968.
Interdisciplinary & Emerging Signature Programs
Research Summary

CENTRAL HYPOTHESIS - We hypothesize that in accordance with the strict temporal and spatial control of NO production in vascular endothelial cells, argininosuccinate synthase, the rate-limiting step of the citrulline-NO cycle, is coordinately and tightly regulated via translational and posttranslational mechanisms, in addition to transcriptional regulation. This premise underlies the rationale for the specific aims our present research which focuses on posttranscriptional events that regulate AS function. First, we hypothesize that the determinants of AS subcellular localization are AS-interacting proteins which guide AS to distinct destinations within the cell. Methodology used to examine this hypothesis employs: 1) immunofluorescence microscopy; 2) pull-down analysis; 3) immunoprecipitation analysis; 4) tandem affinity purification analysis; 5) mass-spectrometry; and 6) NO determinations. Second, we hypothesize that a small protein encoded by an upstream open reading frame (uORF) represses AS mRNA translation under stress conditions by routing stalled AS mRNA particles into discrete cytoplasmic foci known as stress granules. To test this hypothesis we employ: 1) in vitro translation; 2) RNA immunoprecipitation; 3) RNA EMSA; 4) polysome profiling; 5) immunofluorescence microscopy; 6) western blotting; and 7) fluorescence in situ hybridization (FISH). Third, we hypothesize that endothelial AS protein is phosphorylated at serine/theronine residues which mediate an acute response, altering AS activity. To test this hypothesis the following methodology has been employed: 1) proteomics and mass-spectrometry; 2) mutational analysis; 3) NO determinations; 4) AS activity assays; and 5) western blotting. Fourth, to translate our cell culture work to a whole animal system, we are studying how changes in AS expression correlate with impaired vascular endothelial NO production in a diabetic rat model system. Experimental approaches include: 1) animal model characterization; 2) real time RT-PCR; 3) western blotting; 4) NO measurements; 5) immunofluorescence microscopy; and 6) FISH. Overall, results from this research have the potential to distinguish new therapeutic targets to be used to prevent or inhibit vascular endothelial dysfunction found in diabetes, obesity and other related vascular diseases.


Selected Publications

Positions Held
  • Member (Institute for Biomolecular Science, University of South Florida 1985 - 2002)
  • Associate Professor (Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1983 - 1988)
  • Assistant Professor (Department of Biochemistry, College of Medicine, University of South Florida 1977 - 1983)

  • American Heart Association (Member, 2007 - Present)
  • North American Association for the Study of Obesity (Member, 2007 - Present)
  • The RNA Society (Member, 2007 - Present)
  • American Society of Biological Chemists (Member, 2007 - Present)
  • H. Lee Moffitt Cancer Center & Research Institute (Member, 2007 - Present)
  • American Society of Microbiology (Member , 2007 - Present)
  • Institute for Biomolecular Science (Member, 2007 - Present)

  • Medical Education Award, Department of Molecular Medicine (USF College of Medicine, Tampa, FL - 2007)
  • Silver Performance Award for Outstanding Contributions 2006/2007 (USF College of Medicine, Tampa, FL - 2007)
  • Honorary Member of the Golden Key International Honor Society (University of South Florida, Tampa, FL - 2006)
  • Outstanding Contribution to the Program in Undergraduate Medical Education (USF College of Medicine, Tampa, FL - 2003)
  • Presidents Award for Faculty Excellence (USF College of Medicine, Tampa, FL - 2003)
  • Outstanding Contribution to the Program of Undergraduate Medical Education (USF College of Medicine, Tampa, FL - 2001)
  • Professorial Excellence Program Award, 1998-1999. (USF College of Medicine, Tampa, FL - 1999)
  • Distinguished Teacher Award (1996), presented from the Medical Class of 1996. (USF College of Medicine, Tampa, FL - 1996)
  • Outstanding Advisor Award from the Association of Medical Science Graduate Students (USF College of Medicine, Tampa, FL - 1991)
  • The Jerome Krivanek Distinguished Teacher Award (University of South Florida, Tampa, FL - 1991)
  • Outstanding Pre-Clinical Professor (USF College of Medicine, Tampa, FL - 1990)
  • Fellow of the Jane Coffin Childs Memorial Fund for Medical Research (Stanford University, Standord, Calif - 1972)
  • Margaret B. Kraatz Cancer Award for Outstanding Graduate Student Research (UCLA, Los Angeles, Calif - 1972)
  • Graduated with Great Distinction (San Jose State University, San Jose, California - 1968)

Patents & Licensing
  • Polynucleotide Targeted Against the Extended 5'-UTR Region of Argininosuccinate Synthase and Uses Th (USF#-04A060PRC - 2006)

Profile last modified on 10/31/2014