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R Keller,  Ph.D.
Professor, Molecular Medicine


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Email: rkeller@health.usf.edu
Phone: (813) 974-5167
Office: MDC, 3026
Mailing address 12901 Bruce B. Downs Blvd MDC 7
Tampa, FL 33612-4799
  • PHD, Biochemistry, Vanderbilt University , Nashville, United States - 1973.
  • B.S. , Chemistry, Florida Atlantic University ,USA - 1968.
    Interdisciplinary & Emerging Signature Programs
    Research Summary

    Isoprenoid Metabolism

    Personal Page:   www.isoprenoids.com

    Selected Publications
  • Keller, RK.Small, M.Fliesler, SJ. Enzyme blockade: a nonradioactive method to determine the absolute rate of cholesterol synthesis in the brain. Journal of lipid research. 45(10): 1952-7, 2004.
  • Keller, RK.Arnold, TP.Fliesler, SJ. Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome. Journal of lipid research. 45(2): 347-55, 2004.
  • Morgan, D.Keller, RK. What evidence would prove the amyloid hypothesis? Towards rational drug treatments for Alzheimer's disease. Journal of Alzheimer's disease : JAD. 4(3): 257-60, 2002.
  • Keller, RK.Fliesler, SJ. Mechanism of aminobisphosphonate action: characterization of alendronate inhibition of the isoprenoid pathway. Biochemical and biophysical research communications. 266(2): 560-3, 1999.
  • Lopez, D.Chambers, CM.Keller, RK.Ness, GC. Compensatory responses to inhibition of hepatic squalene synthase. Archives of biochemistry and biophysics. 351(2): 159-66, 1998.
  • Hinson, DD.Chambliss, KL.Hoffmann, GF.Krisans, S.Keller, RK.Gibson, KM. Identification of an active site alanine in mevalonate kinase through characterization of a novel mutation in mevalonate kinase deficiency. The Journal of biological chemistry. 272(42): 26756-60, 1997.
  • Fliesler, SJ.Keller, RK. Isoprenoid metabolism in the vertebrate retina. The international journal of biochemistry & cell biology. 29(6): 877-94, 1997.
  • Sobreira, C.Hirano, M.Shanske, S.Keller, RK.Haller, RG.Davidson, E.Santorelli, FM.Miranda, AF.Bonilla, E.Mojon, DS.Barreira, AA.King, MP.DiMauro, S. Mitochondrial encephalomyopathy with coenzyme Q10 deficiency. Neurology. 48(5): 1238-43, 1997.
  • Hoffmann, GF.Wiesmann, UN.Brendel, S.Keller, RK.Gibson, KM. Regulatory adaptation of isoprenoid biosynthesis and the LDL receptor pathway in fibroblasts from patients with mevalonate kinase deficiency. Pediatric research. 41(4 Pt 1): 541-6, 1997.
  • Fliesler, SJ.Boesze-Battaglia, K.Paw, Z.Keller, RK.Albert, AD. Squalene is localized to the plasma membrane in bovine retinal rod outer segments. Experimental eye research. 64(2): 279-82, 1997.
  • Keller, RK. Squalene synthase inhibition alters metabolism of nonsterols in rat liver. Biochimica et biophysica acta. 1303(3): 169-79, 1996.
  • Keller, RK.Zhao, Z.Chambers, C.Ness, GC. Farnesol is not the nonsterol regulator mediating degradation of HMG-CoA reductase in rat liver. Archives of biochemistry and biophysics. 328(2): 324-30, 1996.
  • Fliesler, SJ.Florman, R.Keller, RK. Isoprenoid lipid metabolism in the retina: dynamics of squalene and cholesterol incorporation and turnover in frog rod outer segment membranes. Experimental eye research. 60(1): 57-69, 1995.
  • Fliesler, SJ.Keller, RK. Metabolism of [3H]farnesol to cholesterol and cholesterogenic intermediates in the living rat eye. Biochemical and biophysical research communications. 210(3): 695-702, 1995.
  • Ness, GC.Zhao, Z.Keller, RK. Effect of squalene synthase inhibition on the expression of hepatic cholesterol biosynthetic enzymes, LDL receptor, and cholesterol 7 alpha hydroxylase. Archives of biochemistry and biophysics. 311(2): 277-85, 1994.
  • Keller, RK.Vilsaint, F. Regulation of isoprenoid metabolism in rat liver: near constant chain lengths of dolichyl phosphate and ubiquinone are maintained during greatly altered rates of cholesterogenesis. Biochimica et biophysica acta. 1170(2): 204-10, 1993.
  • Fliesler, SJ.Florman, R.Rapp, LM.Pittler, SJ.Keller, RK. In vivo biosynthesis of cholesterol in the rat retina. FEBS letters. 335(2): 234-8, 1993.
  • Keller, RK.Cannons, A.Vilsaint, F.Zhao, Z.Ness, GC. Identification and regulation of rat squalene synthetase mRNA. Archives of biochemistry and biophysics. 302(1): 304-6, 1993.
  • Keller, RK.Standert, M. GTPases. The Journal of the Florida Medical Association. 79(11): 775-7, 1992.
  • Ness, GC.Keller, RK.Pendleton, LC. Feedback regulation of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase activity by dietary cholesterol is not due to altered mRNA levels. The Journal of biological chemistry. 266(23): 14854-7, 1991.
  • Keller, RK.Fliesler, SJ. Incorporation of squalene into rod outer segments. The Journal of biological chemistry. 265(23): 13709-12, 1990.
  • Margo, CE.Pusateri, TJ.Ulshafer, RJ.Keller, RK. Lipid crystals in malignant melanoma of the choroid. Retina (Philadelphia, Pa.). 10(1): 68-71, 1990.
  • Keller, RK.Nellis, SW.Cuadrado-Simonet, L. A rapid procedure for the separation and analysis of metabolites of the sterol and dolichol pathways. Chemistry and physics of lipids. 51(3-4): 261-7, 1989.
  • Keller, RK.Simonet, WS. Near normal levels of isoprenoid lipids in severe mevalonic aciduria. Biochemical and biophysical research communications. 152(2): 857-61, 1988.
  • Keller, RK.Fliesler, SJ.Nellis, SW. Isoprenoid biosynthesis in the retina. Quantitation of the sterol and dolichol biosynthetic pathways. The Journal of biological chemistry. 263(5): 2250-4, 1988.
  • Keller, RK. The mechanism and regulation of dolichyl phosphate biosynthesis in rat liver. The Journal of biological chemistry. 261(26): 12053-9, 1986.
  • Keller, RK.Adair, WL.Cafmeyer, N.Simion, FA.Fleischer, B.Fleischer, S. Characterization of polyisoprenyl phosphate phosphatase activity in rat liver. Archives of biochemistry and biophysics. 249(1): 207-14, 1986.
  • Keller, RK.Adair, WL.Cafmeyer, N. A procedure for the preparation of [32P]phosphatidic acid. Analytical biochemistry. 155(1): 119-22, 1986.
  • Keller, RK.Nellis, SW. Quantitation of dolichyl phosphate and dolichol in major organs of the rat as a function of age. Lipids. 21(5): 353-5, 1986.
  • Keller, RK.Brennan, S.Cafmeyer, N.Adair, WL. Quantitation of tissue levels of dolichyl pyrophosphate by high-performance liquid chromatography on silica. Journal of chromatography. 343(2): 413-8, 1985.
  • Adair, WL.Keller, RK. Isolation and assay of dolichol and dolichyl phosphate. Methods in enzymology. 111(): 201-15, 1985.
  • Keller, RK.Fuller, MS.Rottler, GD.Connelly, LW. Extraction of dolichyl phosphate and its quantitation by straight-phase high-performance liquid chromatography. Analytical biochemistry. 147(1): 166-73, 1985.
  • Adair, WL.Cafmeyer, N.Keller, RK. Solubilization and characterization of the long chain prenyltransferase involved in dolichyl phosphate biosynthesis. The Journal of biological chemistry. 259(7): 4441-6, 1984.
  • Connelly, LW.Keller, RK. Quantitation of excretion of dolichol and dolichyl phosphate from the rat. Bioscience reports. 4(9): 771-6, 1984.
  • Keller, RK.Armstrong, D.Crum, FC.Koppang, N. Dolichol and dolichyl phosphate levels in brain tissue from English setters with ceroid lipofuscinosis. Journal of neurochemistry. 42(4): 1040-7, 1984.
  • Keller, RK.Rottler, GD.Cafmeyer, N.Adair, WL. Subcellular localization and substrate specificity of dolichol kinase from rat liver. Biochimica et biophysica acta. 719(1): 118-25, 1982.
  • Adair, WL.Keller, RK. Dolichol metabolism in rat liver. Determination of the subcellular distribution of dolichyl phosphate and its site and rate of de novo biosynthesis. The Journal of biological chemistry. 257(15): 8990-6, 1982.
  • Keller, RK.Jehle, E.Adair, WL. The origin of dolichol in the liver of the rat. Determination of the dietary contribution. The Journal of biological chemistry. 257(15): 8985-9, 1982.
  • Keller, RK.Tamkun, JW.Adair, WL. Development of quantitative assay for tissue levels of dolichyl phosphate. Biochemistry. 20(20): 5831-6, 1981.
  • Keller, RK.Swank, GD. Studies on the relative rates of intracellular transport of egg white proteins. Biochimica et biophysica acta. 673(1): 93-100, 1981.
  • Keller, RK.Adair, WL.Ness, GC. Studies on the regulation of glycoprotein biosynthesis. An investigation of the rate-limiting steps of dolichyl phosphate biosynthesis. The Journal of biological chemistry. 254(20): 9966-9, 1979.
  • Keller, RK.Boon, DY.Crum, FC. N-Acetylglucosamine- 1 -phosphate transferase from hen oviduct: solubilization, characterization, and inhibition by tunicamycin. Biochemistry. 18(18): 3946-52, 1979.
  • Keller, RK.Swank, GD. Tunicamycin does not block ovalbumin secretion in the oviduct. Biochemical and biophysical research communications. 85(2): 762-8, 1978.
  • Keller, RK.Adair, WL. Microdetermination of dolichol in tissues. Biochimica et biophysica acta. 489(2): 330-6, 1977.
  • Keller, RK.Chandra, T.Schrader, WT.O'Malley, BW. Protein kinases of the chick oviduct: a study of the cytoplasmic and nuclear enzymes. Biochemistry. 15(9): 1958-67, 1976.
  • Keller, RK.Socher, SH.Krall, JF.Chandra, T.O'Malley, BW. Fractionation of chick oviduct chromatin: IV. Association of protein kinase with transcriptionally active chromatin. Biochemical and biophysical research communications. 66(2): 453-9, 1975.
  • Tulsiani, DR.Keller, RK.Touster, O. The preparation and chemical composition of the multiple forms of beta-glucuronidase from the female rat preputial gland. The Journal of biological chemistry. 250(12): 4770-6, 1975.
  • Keller, RK.Touster, O. Physical and chemical properties of beta-glucuronidase from the preputial gland of the female rat. The Journal of biological chemistry. 250(12): 4765-9, 1975.
  • Keller, RK.Touster, O. Spectral studies of conformational changes induced in beta-glucuronidase by saccharo-1,4-lactone. The Journal of biological chemistry. 250(7): 2739-44, 1975.

  • Positions Held
  • Professor (Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1988 - 2006)
  • Associate Professor (Biochemistry, College of Medicine, University of South Florida 1981 - 1987)
  • Assistant Professor (Biochemistry, College of Medicine, University of South Florida 1975 - 1980)
  • Postdoctoral Fellow (Dept. of Cell Biology, Baylor College of Medicine 1973 - 1975)
  • Graduate Student (Dept. of Biochemistry, Vanderbilt University 1968 - 1972)

  • Memberships
  • Neuroscience Program-USF College of Medicine (Member, 2006 - Present)
  • American Society of Biochemistry and Molecular Biology (ASBMB) (Full Member, 1969 - Present)

  • Awards/Honors
  • Professional Excellence Award (University of South Florida - 1999)
  • National Institutes of Health Research Career Development Award (University of South Florida - 1980)
  • National Institutes of Health Postdoctoral Fellow (Baylor College of Medicine - 1974)
  • National Institutes of Health Predoctoral Trainee (Vanderbilt University - 1968)

  • Profile last modified on 04/09/2012