University of South Florida

University of South Florida


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Kay-Pong Daniel Yip, Ph.D.

Primary Department: COLLEGE OF MEDICINE MOLECULAR PHARMACOLOGY & PHYSIOLOGY

Professor, Department of Molecular Pharmacology and Physiology

Director, Molecular Pharmacology and Physiology Seminar Series

Academic Email : dyip@usf.edu

Phone : (813) 974-7111

Office : MDC, 3034A

Mailing address : 12901 Bruce B. Downs Blvd. MDC 8
Tampa, FL 33612

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Education

  • PHD, Physiology & Biophysics, University Of Southern California, Los Angeles, United States - 1991.
  • B.S. (Honors), Biology, Chinese University of Hong Kong, Hong Kong, Republic of China - 1984.

Research summary

  • The research interests of our laboratory are to develop and apply confocal fluorescence microscopy, laser-based video microscopy, and flash photolysis of caged compounds to study renal cellular function in situ and in vivo. Currently we are investigating the regulation of water channels (aquaporin-2) trafficking in kidney collecting duct by antidiuretic hormone (vasopressin), the role of integrins and Ca2+ microdomains in mechanical signal transduction in renal vascular smooth muscle, and cellular mechanisms of pressure natriuresis and its relationship with the development of hypertension.

    In addition, we're studying the regulation of blood pressure and renal functions, with emphasis in developing novel imaging techniques for in vivo and in situ studies, and using interdisciplinary approaches to study the physiological phenomena from subcellular to organ level. There are two active projects in lab, which are supported by NIH and AHA.

    1. Intracellular Ca2+ oscillations and trafficking of aquaporin-2 (AQP2) in intact inner medullary collecting duct cells. Confocal fluorescence microscopy allows the study of physiological process in a single cell in a perfused tubule without the complication of out-of-focus-fluorescence signal derived from the other cells. By combining confocal fluorescence microscopy and tubular microperfusion technique, we are the first to demonstrate that vasopressin (AVP) in physiological range ( 0.01  0.1 nM) mobilizes intracellular Ca2+ in form of oscillations in intact inner medullary collecting duct (J. Biol. Chem. 2000). By developing a novel confocal fluorescence imaging technique using a styryl dye (FM1-43) to monitor real time exocytosis of aquaporin-2 in inner medullary collecting duct (IMCD), we provide the first direct evidence that AVP induced AQP2 exocytotic insertion is Ca2+ dependent (J. Physiol. 2002). We also have evidence that AVP uses not only PKA but also EPAC as cAMP receptor to induce AQP2 apical exocytosis (Am. J. Physiol. 2005).

    2. Integrin-mediated mechanotransduction in renal vascular smooth muscle cells. Myogenic response and tubuloglomerular feedback are the two major mechanisms responsible to provide renal blood flow autoregulation. But mechanism of mechanotransduction in myogenic response is not known. We hypothesize that increase of transmural pressure (stretch) alters the attachment between extracellular matrix (ECM) and integrins in vascular smooth muscle, and that mechanical force is transduced via integrin to trigger Ca2+ sparks (local subcellular Ca2+ release from ryanodine receptors, detected by high speed confocal microscopy) to initiate and maintain myogenic contraction. We are testing these hypotheses by applying controlled mechanical force using fibronectin-coated paramagnetic beads (or nanoparticles) and monitoring the occurrence of Ca2+ sparks in freshly isolated renal vascular smooth muscles, and examining the effects of transmural pressure in the frequency of Ca2+ sparks in intact renal vascular smooth muscle cells. (Am. J. Physiol 2007)

Selected Publications

Positions Held

Interdisciplinary and Emerging Signature Programs

  • Allergy, Immunology & Infectious Disease, Cancer Biology, Cardiovascular, Cellular and Molecular Biology, Environmental & Global Health, Pulmonary Physiology.

Memberships

  • American Society of Nephrology (Member, 2008 - Present)
  • American Physiological Society (Member, 2008 - Present)

Awards/Honors

  • National Kidney Foundation Young Investigator Grant (National Kidney Foundation - 1994)
  • Clifford and Evelyn Cherry Fellowship Award (American Heart Association - 1992)
  • American Heart Association Postdoctoral Fellowship (American Heart Association - 1992)

Lectures

  • "Integrin-mediated mechanotransduction in myogenic response of afferent arterioles" 2008 Experiment Biology symposium. FL, United States - 2008.
  • "The Kidney: Cellular, Tubular, and Vascular Physiology" Mathematical Biosciences Institute of Ohio State University, Workshop . OH, United States - 2007.
  • "Calcium signaling in aquaporin trafficking" The 5th International Conference of Aquaporin . Japan - 2007.
  • "Calcium signaling and Aquaporin-2 trafficking in kidney collecting duct" Microscopy and Microanalysis 2007 invited speaker. FL, United States - 2007.
  • "Trafficking of aquaporin-2 in kidney inner medullary collecting duct" Chinese University of Hong Kong, Department of Physiology . Japan - 2007.
  • "Bifurcation of renal hemodynamics and pressure natriuresis" University of Florida, Department of Medicine Division of Nephrology . FL, United States - 2005.

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Profile last modified on 10/31/2019