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Michael White,  Ph.D.
Deputy Director, Florida Center for Drug Discovery and Innovation
Professor, Departments of Molecular Medicine & Global Health


Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

Professor
COLLEGE OF MEDICINE MOLECULAR MEDICINE

Professor
COPH GLOBAL HEALTH

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Contacts
Email: mwhite3@health.usf.edu
Phone: (813) 974-8411
Office: IDRB, 325
Mailing address Dept. of Molecular Medicine 3720 Spectrum Blvd, Suite 304
Tampa, FL 33612
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Education
  • post-doc, Biochemistry, University of Washington ,USA - 1988.
  • PHD, Microbiolgy, Oregon State University , United States - 1984.
  • Ph.D., Microbiology, Oregon State University ,USA - 1983.
  • B.A., Microbiology, San Jose State University ,USA - 1978.
  •  
    Interdisciplinary & Emerging Signature Programs
    Allergy, Immunology & Infectious Disease.
     
    Research Summary

    Research in the White laboratory is focused on how the malaria-related protozoa, Toxoplasma gondii, grows and develops in its human host. T. gondii is an environmental pathogen of the central nervous system (class B bioterrorism agent), which causes an often fatal disease in people who are immunocompromised. Projects in our laboratory focus on how these parasites interact with their host cell to produce new progeny and establish persistent infections. Our studies employ a diverse and extensive array of genetic and biochemical approaches with special emphasis on whole-cell strategies that enable a global understanding of the biochemical pathways involved in parasite growth and development. From forward genetic approaches that allow essential protein factors to be identified by genetic complementation to cataloging gene clusters within the whole-cell transcriptome that are responsible for transduction of the parasite cell division cycle, we are uncovering novel pathways that hold the key to important parasite biology.

     


    Selected Publications
  • White, MW.Radke, JR.Radke, JB. Toxoplasma development-turn the switch on or off? Cellular microbiology. (): , 2014.
  • Radke, JB.Lucas, O.De Silva, EK.Ma, Y.Sullivan, WJ.Weiss, LM.Llinas, M.White, MW. ApiAP2 transcription factor restricts development of the Toxoplasma tissue cyst. Proceedings of the National Academy of Sciences of the United States of America. 110(17): 6871-6, 2013.
  • Suvorova, ES.Croken, M.Kratzer, S.Ting, LM.de Felipe, MC.Balu, B.Markillie, ML.Weiss, LM.Kim, K.White, MW. Discovery of a splicing regulator required for cell cycle progression. PLoS genetics. 9(2): e1003305, 2013.
  • Suvorova, ES.Radke, JB.Ting, LM.Vinayak, S.Alvarez, CA.Kratzer, S.Kim, K.Striepen, B.White, MW. A nucleolar AAA-NTPase is required for parasite division. Molecular microbiology. 90(2): 338-55, 2013.
  • Gaji, R., Behnke, M.S., Lehmann, M.M., White, M.W., and Carruthers, V.B. Parasite egress and invasion are tied to early G1 to ensure efficient cell-to-cell transmission. Mol. Microbiol.. 79(): 192-204, 2011.
  • Suvorova, E. S., Lehmann, M.W., Kratzer, S., White, M.W. Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii Molecular Biochemical Parasitology. in press(): , 2011.
  • Sheiner, L. Piester, J., Poulsen, N., Beatty, W. , Behnke, M., Lucas, O. , Kroger, N., White, M.W., Striepen, B. In silico investigation and in vivo characterization of proteins of the T. gondii apicoplast PLoS Pathogens. in press(): , 2011.
  • Szatanek, T., Anderson-White, B. R., White, M. W., Saeij, J., Gubbels, M. J. Cactin is essential for G1 progression in Toxoplasma gondii Molecular Microbiology. in review(): , 2011.
  • Behnke, M., Wootton, J., Lehmann, M., Radke, J.B., Lucas, O., Nawas, J., Sibley, L. D., and White, M. W. Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii. [FF10a rating by Faculty of 1000] PLoS One . pii: e12354(): , 2010.
  • Amit Bahl, A., Davis, P.H., Behnke, M., Dzierszinski, F., Jagalur, M., Chen, F. Dhanasekaran Shanmugam, D., White, M., Kulp, D. and Roos, D. S. A novel multifunctional oligonucleotide microarray for Toxoplasma gondii. BMC Genomics. 11(): 603-607, 2010.
  • Asis Khan, A. Behnke, M.S., Dunay, I.R., White, M.W., and Sibley, L.D. Phenotypic and gene expression changes among type I strains of Toxoplasma gondii. Eurkaryotic Cell . 8(12): 1828-1836, 2009.
  • Conde de Felipe, MM.Lehmann, MM.Jerome, ME.White, MW. Inhibition of Toxoplasma gondii growth by pyrrolidine dithiocarbamate is cell cycle specific and leads to population synchronization. Molecular and biochemical parasitology. 157(1): 22-31, 2008.
  • Gubbels, M., White, M.W., and Szatanek, T. The cell cycle and control of Toxoplasma division: tightly knit or loosely stitched. Int. J. Parasitol.. 38(12): 1343-1358, 2008.
  • Gubbels, MJ.Lehmann, M.Muthalagi, M.Jerome, ME.Brooks, CF.Szatanek, T.Flynn, J.Parrot, B.Radke, J.Striepen, B.White, MW. Forward genetic analysis of the apicomplexan cell division cycle in Toxoplasma gondii. [FF10a rating by Faculty of 1000] PLoS pathogens. 4(2): e36, 2008.
  • Behnke, MS.Radke, JB.Smith, AT.Sullivan, WJ.White, MW. The transcription of bradyzoite genes in Toxoplasma gondii is controlled by autonomous promoter elements. Molecular microbiology. 68(6): 1502-18, 2008.
  • Saeij, JP.Coller, S.Boyle, JP.Jerome, ME.White, MW.Boothroyd, JC. Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. [FF17a rating by Faculty of 1000] Nature. 445(7125): 324-7, 2007.
  • Radke, JR.Donald, RG.Eibs, A.Jerome, ME.Behnke, MS.Liberator, P.White, MW. Changes in the expression of human cell division autoantigen-1 influence Toxoplasma gondii growth and development. PLoS pathogens. 2(10): e105, 2006.
  • Taylor, S., Barragan, A., Su, C., Fux, B., Fentress, S., Tang, K., Beatty, W.L., El Hajj, H., Jerome, M., Behnke, M. S., White, M.W., Wootton, J.C., and Sibley, L.D. A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. [FF14a rating by Faculty of 1000] Science. 314(): 1776-1780, 2006.
  • Boyle, JP.Rajasekar, B.Saeij, JP.Ajioka, JW.Berriman, M.Paulsen, I.Roos, DS.Sibley, LD.White, MW.Boothroyd, JC. Just one cross appears capable of dramatically altering the population biology of a eukaryotic pathogen like Toxoplasma gondii. [FF8a rating by Faculty of 1000] Proceedings of the National Academy of Sciences of the United States of America. 103(27): 10514-9, 2006.
  • Radke, JR.Behnke, MS.Mackey, AJ.Radke, JB.Roos, DS.White, MW. The transcriptome of Toxoplasma gondii. BMC biology. 3(): 26, 2005.
  • Guerini, MN.Behnke, MS.White, MW. Biochemical and genetic analysis of the distinct proliferating cell nuclear antigens of Toxoplasma gondii. Molecular and biochemical parasitology. 142(1): 56-65, 2005.
  • White, MW.Jerome, ME.Vaishnava, S.Guerini, M.Behnke, M.Striepen, B. Genetic rescue of a Toxoplasma gondii conditional cell cycle mutant. Molecular microbiology. 55(4): 1060-71, 2005.
  • Radke, JR.Gubbels, MJ.Jerome, ME.Radke, JB.Striepen, B.White, MW. Identification of a sporozoite-specific member of the Toxoplasma SAG superfamily via genetic complementation. Molecular microbiology. 52(1): 93-105, 2004.
  • Radke, JR.Guerini, MN.Jerome, M.White, MW. A change in the premitotic period of the cell cycle is associated with bradyzoite differentiation in Toxoplasma gondii. Molecular and biochemical parasitology. 131(2): 119-27, 2003.
  • Mitzel, DN.Weisend, CM.White, MW.Hardy, ME. Translational regulation of rotavirus gene expression. The Journal of general virology. 84(Pt 2): 383-91, 2003.
  • Striepen, B.White, MW.Li, C.Guerini, MN.Malik, SB.Logsdon, JM.Liu, C.Abrahamsen, MS. Genetic complementation in apicomplexan parasites. Proceedings of the National Academy of Sciences of the United States of America. 99(9): 6304-9, 2002.
  • Kvaal, CA.Radke, JR.Guerini, MN.White, MW. Isolation of a Toxoplasma gondii cyclin by yeast two-hybrid interactive screen. Molecular and biochemical parasitology. 120(2): 187-94, 2002.
  • Radke, JR.Striepen, B.Guerini, MN.Jerome, ME.Roos, DS.White, MW. Defining the cell cycle for the tachyzoite stage of Toxoplasma gondii. Molecular and biochemical parasitology. 115(2): 165-75, 2001.
  • Radke, JR.Guerini, MN.White, MW. Toxoplasma gondii: characterization of temperature-sensitive tachyzoite cell cycle mutants. Experimental parasitology. 96(3): 168-77, 2000.
  • Guerini, MN.Que, X.Reed, SL.White, MW. Two genes encoding unique proliferating-cell-nuclear-antigens are expressed in Toxoplasma gondii. Molecular and biochemical parasitology. 109(2): 121-31, 2000.
  • Radke, JR.White, MW. Expression of herpes simplex virus thymidine kinase in Toxoplasma gondii attenuates tachyzoite virulence in mice. Infection and immunity. 67(10): 5292-7, 1999.
  • Radke, JR.White, MW. A cell cycle model for the tachyzoite of Toxoplasma gondii using the Herpes simplex virus thymidine kinase. Molecular and biochemical parasitology. 94(2): 237-47, 1998.
  • Jerome, ME.Radke, JR.Bohne, W.Roos, DS.White, MW. Toxoplasma gondii bradyzoites form spontaneously during sporozoite-initiated development. Infection and immunity. 66(10): 4838-44, 1998.
  • Bohne, W.Hunter, CA.White, MW.Ferguson, DJ.Gross, U.Roos, DS. Targeted disruption of the bradyzoite-specific gene BAG1 does not prevent tissue cyst formation in Toxoplasma gondii. Molecular and biochemical parasitology. 92(2): 291-301, 1998.
  • Tilley, M.Fichera, ME.Jerome, ME.Roos, DS.White, MW. Toxoplasma gondii sporozoites form a transient parasitophorous vacuole that is impermeable and contains only a subset of dense-granule proteins. Infection and immunity. 65(11): 4598-605, 1997.
  • White, MW.Radke, JR. Methods to prepare RNA and to isolate developmentally regulated genes from Eimeria. Methods (San Diego, Calif.). 13(2): 158-70, 1997.
  • Clark, TG.Abrahamsen, MS.White, MW. Developmental expression of heat shock protein 90 in Eimeria bovis. Molecular and biochemical parasitology. 78(1-2): 259-63, 1996.
  • Severson, WE.Mascolo, PL.White, MW. Lymphocyte p56L32 is a RNA/DNA-binding protein which interacts with conserved elements of the murine L32 ribosomal protein mRNA. European journal of biochemistry / FEBS. 229(2): 426-32, 1995.
  • Abrahamsen, MS.Clark, TG.White, MW. An improved method for isolating RNA from coccidian oocysts. The Journal of parasitology. 81(1): 107-9, 1995.
  • Abrahamsen, MS.Johnson, RR.Hathaway, M.White, MW. Identification of Eimeria bovis merozoite cDNAs using differential mRNA display. Molecular and biochemical parasitology. 71(2): 183-91, 1995.
  • Speer, CA.Tilley, M.Temple, ME.Blixt, JA.Dubey, JP.White, MW. Sporozoites of Toxoplasma gondii lack dense-granule protein GRA3 and form a unique parasitophorous vacuole. Molecular and biochemical parasitology. 75(1): 75-86, 1995.
  • Abrahamsen, MS.Johnson, RR.Clark, TG.White, MW. Developmental regulation of an Eimeria bovis mRNA encoding refractile body-associated proteins. Molecular and biochemical parasitology. 68(1): 25-34, 1994.
  • Abrahamsen, MS.Johnson, RR.Jutila, MA.Speer, CA.White, MW. Eimeria bovis: expression of a related group of refractile body-associated proteins during schizogony. Experimental parasitology. 78(3): 331-5, 1994.
  • Abrahamsen, MS.Clark, TG.Mascolo, P.Speer, CA.White, MW. Developmental gene expression in Eimeria bovis. Molecular and biochemical parasitology. 57(1): 1-14, 1993.
  • White, MW.Shatry, AM.Jutila, MA.Speer, CA. Biochemical and ultrastructural observations of coccidian parasite and host cell interactions. Sub-cellular biochemistry. 18(): 365-83, 1992.

  • Positions Held
  • Affiliate WWAMI Professor (Department of Biochemistry, University of Washington 2003 - 2009)
  • Professor (Veterinary Molecular Biology, Montana State University 2001 - 2009)
  • Visiting Professor (Department of Biology, University of Pennsylvania 1996 - 2004)
  • Associate Professor (Veterinary Molecular Biology, Montana State University 1994 - 2001)
  • Assistant Professor (Veterinary Molecular Biology, Montana State University 1988 - 1994)
  • Research Assistant & Professor (Department of Biochemistry, University of Washington 1988 - 1988)
  • Senior Fellow (Department of Biochemistry, University of Washington 1984 - 1987)


  • Profile last modified on 09/16/2013