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Denise Cooper,  Ph.D.
Research Career Scientist,
Professor, Dept. of Molecular Medicine, College of Medicine


Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

Professor
COLLEGE OF MEDICINE MOLECULAR MEDICINE

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Contacts
Email: dcooper@health.usf.edu
Office: VAH, 2-214
Mailing address James A. Haley Veterans' Hospital 13000 Bruce B. Downs Blvd., room 2-214
Tampa, FL 33612
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Education
  • MS, Biochemistry and Molecular Biology, University of Oklahoma Health Sciences ,USA - 2006.
  • PHD, Biochemistry and Molecular Biology, University Of Oklahoma Health Sciences Center , Oklahoma City, United States - 1983.
  •  
    Interdisciplinary & Emerging Signature Programs
    Metabolic Regulation and Disorders.
     


    Selected Publications
  • Sampson, SR.Cooper, DR. Specific protein kinase C isoforms as transducers and modulators of insulin signaling. Molecular genetics and metabolism. 89(1-2): 32-47, .
  • Jiang, K.Apostolatos, AH.Ghansah, T.Watson, JE.Vickers, T.Cooper, DR.Epling-Burnette, PK.Patel, NA. Identification of a novel antiapoptotic human protein kinase C delta isoform, PKCdeltaVIII in NT2 cells. Biochemistry. 47(2): 787-97, 2008.
  • Ghosh, N.Patel, N.Jiang, K.Watson, JE.Cheng, J.Chalfant, CE.Cooper, DR. Ceramide-activated protein phosphatase involvement in insulin resistance via Akt, serine/arginine-rich protein 40, and ribonucleic acid splicing in L6 skeletal muscle cells. Endocrinology. 148(3): 1359-66, 2007.
  • Horovitz-Fried, M.Jacob, AI.Cooper, DR.Sampson, SR. Activation of the nuclear transcription factor SP-1 by insulin rapidly increases the expression of protein kinase C delta in skeletal muscle. Cellular signalling. 19(3): 556-62, 2007.
  • Patel, NA.Song, SS.Cooper, DR. PKCdelta alternatively spliced isoforms modulate cellular apoptosis in retinoic acid-induced differentiation of human NT2 cells and mouse embryonic stem cells. Gene expression. 13(2): 73-84, 2006.
  • Horovitz-Fried, M.Cooper, DR.Patel, NA.Cipok, M.Brand, C.Bak, A.Inbar, A.Jacob, AI.Sampson, SR. Insulin rapidly upregulates protein kinase Cdelta gene expression in skeletal muscle. Cellular signalling. 18(2): 183-93, 2006.
  • Patel, NA.Kaneko, S.Apostolatos, HS.Bae, SS.Watson, JE.Davidowitz, K.Chappell, DS.Birnbaum, MJ.Cheng, JQ.Cooper, DR. Molecular and genetic studies imply Akt-mediated signaling promotes protein kinase CbetaII alternative splicing via phosphorylation of serine/arginine-rich splicing factor SRp40. The Journal of biological chemistry. 280(14): 14302-9, 2005.
  • Patel, NA.Apostolatos, HS.Mebert, K.Chalfant, CE.Watson, JE.Pillay, TS.Sparks, J.Cooper, DR. Insulin regulates protein kinase CbetaII alternative splicing in multiple target tissues: development of a hormonally responsive heterologous minigene. Molecular endocrinology (Baltimore, Md.). 18(4): 899-911, 2004.
  • Patel, NA.Eichler, DC.Chappell, DS.Illingworth, PA.Chalfant, CE.Yamamoto, M.Dean, NM.Wyatt, JR.Mebert, K.Watson, JE.Cooper, DR. The protein kinase C beta II exon confers mRNA instability in the presence of high glucose concentrations. The Journal of biological chemistry. 278(2): 1149-57, 2003.
  • Patel, NA.Yamamoto, M.Illingworth, P.Mancu, D.Mebert, K.Chappell, DS.Watson, JE.Cooper, DR. Phosphoinositide 3-kinase mediates protein kinase C beta II mRNA destabilization in rat A10 smooth muscle cell cultures exposed to high glucose. Archives of biochemistry and biophysics. 403(1): 111-20, 2002.
  • Jenq, W.Ramirez, G.Peguero, A.Cooper, DR.Vesely, DL. D-glucose and NaCl enhance the expression of aquaporin-1: inhibition of both by cholera toxin. Nephron. 92(2): 279-86, 2002.
  • Okumura, M.Yamamoto, M.Sakuma, H.Kojima, T.Maruyama, T.Jamali, M.Cooper, DR.Yasuda, K. Leptin and high glucose stimulate cell proliferation in MCF-7 human breast cancer cells: reciprocal involvement of PKC-alpha and PPAR expression. Biochimica et biophysica acta. 1592(2): 107-16, 2002.
  • Patel, NA.Chalfant, CE.Watson, JE.Wyatt, JR.Dean, NM.Eichler, DC.Cooper, DR. Insulin regulates alternative splicing of protein kinase C beta II through a phosphatidylinositol 3-kinase-dependent pathway involving the nuclear serine/arginine-rich splicing factor, SRp40, in skeletal muscle cells. The Journal of biological chemistry. 276(25): 22648-54, 2001.
  • Sanchez-Ramos, J.Song, S.Cardozo-Pelaez, F.Hazzi, C.Stedeford, T.Willing, A.Freeman, TB.Saporta, S.Janssen, W.Patel, N.Cooper, DR.Sanberg, PR. Adult bone marrow stromal cells differentiate into neural cells in vitro. Experimental neurology. 164(2): 247-56, 2000.
  • Chalfant, CE.Ciaraldi, TP.Watson, JE.Nikoulina, S.Henry, RR.Cooper, DR. Protein kinase Ctheta expression is increased upon differentiation of human skeletal muscle cells: dysregulation in type 2 diabetic patients and a possible role for protein kinase Ctheta in insulin-stimulated glycogen synthase activity. Endocrinology. 141(8): 2773-8, 2000.
  • Yamamoto, M.Acevedo-Duncan, M.Chalfant, CE.Patel, NA.Watson, JE.Cooper, DR. Acute glucose-induced downregulation of PKC-betaII accelerates cultured VSMC proliferation. American journal of physiology. Cell physiology. 279(3): C587-95, 2000.
  • Patel, NA.Chalfant, CE.Yamamoto, M.Watson, JE.Eichler, DC.Cooper, DR. Acute hyperglycemia regulates transcription and posttranscriptional stability of PKCbetaII mRNA in vascular smooth muscle cells. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 13(1): 103-13, 1999.
  • Yan, SF.Lu, J.Zou, YS.Soh-Won, J.Cohen, DM.Buttrick, PM.Cooper, DR.Steinberg, SF.Mackman, N.Pinsky, DJ.Stern, DM. Hypoxia-associated induction of early growth response-1 gene expression. The Journal of biological chemistry. 274(21): 15030-40, 1999.
  • Jenq, W.Cooper, DR.Bittle, P.Ramirez, G. Aquaporin-1 expression in proximal tubule epithelial cells of human kidney is regulated by hyperosmolarity and contrast agents. Biochemical and biophysical research communications. 256(1): 240-8, 1999.
  • Yamamoto, M.Patel, NA.Taggart, J.Sridhar, R.Cooper, DR. A shift from normal to high glucose levels stimulates cell proliferation in drug sensitive MCF-7 human breast cancer cells but not in multidrug resistant MCF-7/ADR cells which overproduce PKC-betaII. International journal of cancer. Journal international du cancer. 83(1): 98-106, 1999.
  • Cooper, DR.Watson, JE.Patel, N.Illingworth, P.Acevedo-Duncan, M.Goodnight, J.Chalfant, CE.Mischak, H. Ectopic expression of protein kinase CbetaII, -delta, and -epsilon, but not -betaI or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells. Archives of biochemistry and biophysics. 372(1): 69-79, 1999.
  • Perrotti, D.Bonatti, S.Trotta, R.Martinez, R.Skorski, T.Salomoni, P.Grassilli, E.Lozzo, RV.Cooper, DR.Calabretta, B. TLS/FUS, a pro-oncogene involved in multiple chromosomal translocations, is a novel regulator of BCR/ABL-mediated leukemogenesis. The EMBO journal. 17(15): 4442-55, 1998.
  • Yamamoto, M.Acevedo-Duncan, M.Chalfant, CE.Patel, NA.Watson, JE.Cooper, DR. The roles of protein kinase C beta I and beta II in vascular smooth muscle cell proliferation. Experimental cell research. 240(2): 349-58, 1998.
  • Chalfant, CE.Watson, JE.Bisnauth, LD.Kang, JB.Patel, N.Obeid, LM.Eichler, DC.Cooper, DR. Insulin regulates protein kinase CbetaII expression through enhanced exon inclusion in L6 skeletal muscle cells. A novel mechanism of insulin- and insulin-like growth factor-i-induced 5' splice site selection. The Journal of biological chemistry. 273(2): 910-6, 1998.
  • Acevedo-Duncan, M.Zhang, R.Cooper, DR.Greenberg, HM. Effects of interferon and PKC modulators on human glioma protein kinase C, cell proliferation, and cell cycle. Neurochemical research. 22(7): 775-84, 1997.
  • Jenq, W.Cooper, DR.Ramirez, G. Integrin expression on cell adhesion function and up-regulation of P125FAK and paxillin in metastatic renal carcinoma cells. Connective tissue research. 34(3): 161-74, 1996.
  • Chalfant, CE.Ohno, S.Konno, Y.Fisher, AA.Bisnauth, LD.Watson, JE.Cooper, DR. A carboxy-terminal deletion mutant of protein kinase C beta II inhibits insulin-stimulated 2-deoxyglucose uptake in L6 rat skeletal muscle cells. Molecular endocrinology (Baltimore, Md.). 10(10): 1273-81, 1996.
  • Coulson, R.Proch, PS.Olsson, RA.Chalfant, CE.Cooper, DR. Upregulated renal adenosine A1 receptors augment PKC and glucose transport but inhibit proliferation. The American journal of physiology. 270(2 Pt 2): F263-74, 1996.
  • Yamada, K.Avignon, A.Standaert, ML.Cooper, DR.Spencer, B.Farese, RV. Effects of insulin on the translocation of protein kinase C-theta and other protein kinase C isoforms in rat skeletal muscles. The Biochemical journal. 308 ( Pt 1)(): 177-80, 1995.
  • Chalfant, CE.Mischak, H.Watson, JE.Winkler, BC.Goodnight, J.Farese, RV.Cooper, DR. Regulation of alternative splicing of protein kinase C beta by insulin. The Journal of biological chemistry. 270(22): 13326-32, 1995.
  • Acevedo-Duncan, M.Collins, J.Zhang, R.Haller, E.Chalfant, CE.Cooper, DR. In situ effects of interferon on human glioma protein kinase C-alpha and -beta ultrastructural localization. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research. 6(11): 1353-65, 1995.
  • Farese, RV.Standaert, ML.Arnold, TP.Yamada, K.Musunuru, K.Hernandez, H.Mischak, H.Cooper, DR. Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes. The Journal of clinical investigation. 93(5): 1894-9, 1994.
  • Yamada, K.Standaert, ML.Yu, B.Mischak, H.Cooper, DR.Farese, RV. Insulin-like effects of sodium orthovanadate on diacylglycerol-protein kinase C signaling in BC3H-1 myocytes. Archives of biochemistry and biophysics. 312(1): 167-72, 1994.
  • Zhao, L.Standaert, ML.Cooper, DR.Avignon, A.Farese, RV. Effects of insulin on protein kinase-C (PKC) in HIRC-B cells: specific activation of PKC epsilon and its resistance to phorbol ester-induced down-regulation. Endocrinology. 135(6): 2504-10, 1994.
  • Farese, RV.Standaert, ML.Yamada, K.Huang, LC.Zhang, C.Cooper, DR.Wang, Z.Yang, Y.Suzuki, S.Toyota, T. Insulin-induced activation of glycerol-3-phosphate acyltransferase by a chiro-inositol-containing insulin mediator is defective in adipocytes of insulin-resistant, type II diabetic, Goto-Kakizaki rats. Proceedings of the National Academy of Sciences of the United States of America. 91(23): 11040-4, 1994.
  • Standaert, ML.Musunuru, K.Yamada, K.Cooper, DR.Farese, RV. Insulin-stimulated phosphatidylcholine hydrolysis, diacylglycerol/protein kinase C signalling, and hexose transport in pertussis toxin-treated BC3H-1 myocytes. Cellular signalling. 6(6): 707-16, 1994.
  • Gower, WR.Dietz, JR.Vesely, DL.Finley, CL.Skolnick, KA.Fabri, PJ.Cooper, DR.Chalfant, CE. Atrial natriuretic peptide gene expression in the rat gastrointestinal tract. Biochemical and biophysical research communications. 202(1): 562-70, 1994.
  • Farese, RV.Standaert, M.Yu, B.Hernandez, H.Cooper, DR. 2-Hydroxypropyl-beta-cyclodextrin enhances phorbol ester effects on glucose transport and/or protein kinase C-beta translocation to the plasma membrane in rat adipocytes and soleus muscles. The Journal of biological chemistry. 268(27): 19949-55, 1993.
  • Cooper, DR.Khalakdina, A.Watson, JE. Chronic effects of glucose on insulin signaling in A-10 vascular smooth muscle cells. Archives of biochemistry and biophysics. 302(2): 490-8, 1993.
  • Standaert, ML.Cooper, DR.Hernandez, H.Arnold, TP.Farese, RV. Differential down-regulation of insulin-sensitive protein kinase-C isoforms by 12-O-tetradecanoylphorbol-13-acetate in rat adipocytes and BC3H-1 myocytes. Endocrinology. 132(2): 689-92, 1993.
  • Cooper, DR.Watson, JE.Dao, ML. Decreased expression of protein kinase-C alpha, beta, and epsilon in soleus muscle of Zucker obese (fa/fa) rats. Endocrinology. 133(5): 2241-7, 1993.
  • Arnold, TP.Standaert, ML.Hernandez, H.Watson, J.Mischak, H.Kazanietz, MG.Zhao, L.Cooper, DR.Farese, RV. Effects of insulin and phorbol esters on MARCKS (myristoylated alanine-rich C-kinase substrate) phosphorylation (and other parameters of protein kinase C activation) in rat adipocytes, rat soleus muscle and BC3H-1 myocytes. The Biochemical journal. 295 ( Pt 1)(): 155-64, 1993.
  • Farese, RV.Standaert, ML.Cooper, DR. Protein kinase C downregulation? Nature. 360(6402): 305, 1992.
  • Farese, RV.Standaert, ML.Francois, AJ.Ways, K.Arnold, TP.Hernandez, H.Cooper, DR. Effects of insulin and phorbol esters on subcellular distribution of protein kinase C isoforms in rat adipocytes. The Biochemical journal. 288 ( Pt 1)(): 319-23, 1992.
  • Farese, RV.Standaert, ML.Arnold, T.Yu, B.Ishizuka, T.Hoffman, J.Vila, M.Cooper, DR. The role of protein kinase C in insulin action. Cellular signalling. 4(2): 133-43, 1992.
  • Cooper, DR.Watson, JE.Hernandez, H.Yu, B.Standaert, ML.Ways, DK.Arnold, TT.Ishizuka, T.Farese, RV. Direct evidence for protein kinase C involvement in insulin-stimulated hexose uptake. Biochemical and biophysical research communications. 188(1): 142-8, 1992.
  • Yu, B.Standaert, M.Arnold, T.Hernandez, H.Watson, J.Ways, K.Cooper, DR.Farese, RV. Effects of insulin on diacylglycerol/protein kinase-C signalling and glucose transport in rat skeletal muscles in vivo and in vitro. Endocrinology. 130(6): 3345-55, 1992.
  • Coulson, R.Johnson, RA.Olsson, RA.Cooper, DR.Scheinman, SJ. Adenosine stimulates phosphate and glucose transport in opossum kidney epithelial cells. The American journal of physiology. 260(6 Pt 2): F921-8, 1991.
  • Farese, RV.Ishizuka, T.Standaert, ML.Cooper, DR. Sulfonylureas activate glucose transport and protein kinase C in rat adipocytes. Metabolism: clinical and experimental. 40(2): 196-200, 1991.
  • Standaert, ML.Sasse, J.Cooper, DR.Farese, RV. Protein kinase C(19-31) pseudosubstrate inhibition of insulin action in rat adipocytes. FEBS letters. 282(1): 139-42, 1991.
  • Farese, RV.Standaert, ML.Ishizuka, T.Yu, B.Hernandez, H.Waldron, C.Watson, J.Farese, JP.Cooper, DR.Wickstrom, E. Antisense DNA downregulates protein kinase C isozymes (beta and alpha) and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes. Antisense research and development. 1(1): 35-42, 1991.
  • Farese, RV.Standaert, ML.Ishizuka, T.Yu, B.Arnold, TP.Cooper, DR. The role of diacylglycerol/protein kinase C signaling in insulin-stimulated glucose transport. Transactions of the Association of American Physicians. 104(): 187-93, 1991.
  • Ishizuka, T.Cooper, DR.Arnold, T.Hernandez, H.Farese, RV. Downregulation of protein kinase C and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes by phorbol esters, glucose, and insulin. Diabetes. 40(10): 1274-81, 1991.
  • Cooper, DR.Ishizuka, T.Watson, JE.Standaert, ML.Nair, G.Farese, RV. Protein kinase C activation patterns are determined by methodological variations. Studies of insulin action in BC3H-1 myocytes and rat adipose tissue. Biochimica et biophysica acta. 1054(1): 95-102, 1990.
  • Standaert, ML.Buckley, DJ.Ishizuka, T.Hoffman, JM.Cooper, DR.Pollet, RJ.Farese, RV. Protein kinase C inhibitors block insulin and PMA-stimulated hexose transport in isolated rat adipocytes and BC3H-1 myocytes. Metabolism: clinical and experimental. 39(11): 1170-9, 1990.
  • Cooper, DR.Vila, MC.Watson, JE.Nair, G.Pollet, RJ.Standaert, M.Farese, RV. Sulfonylurea-stimulated glucose transport association with diacylglycerollike activation of protein kinase C in BC3H1 myocytes. Diabetes. 39(11): 1399-407, 1990.
  • Ishizuka, T.Cooper, DR.Farese, RV. Insulin provokes apparent increases in rat adipocyte M-kinase. Biochemical and biophysical research communications. 169(3): 966-72, 1990.
  • Ishizuka, T.Cooper, DR.Hernandez, H.Buckley, D.Standaert, M.Farese, RV. Effects of insulin on diacylglycerol-protein kinase C signaling in rat diaphragm and soleus muscles and relationship to glucose transport. Diabetes. 39(2): 181-90, 1990.
  • Cooper, DR.Hernandez, H.Kuo, JY.Farese, RV. Insulin increases the synthesis of phospholipid and diacylglycerol and protein kinase C activity in rat hepatocytes. Archives of biochemistry and biophysics. 276(2): 486-94, 1990.
  • Farese, RV.Cooper, DR. Potential role of phospholipid-signaling systems in insulin action and states of clinical insulin resistance. Diabetes/metabolism reviews. 5(5): 455-74, 1989.
  • Farese, RV.Nair, GP.Sierra, CG.Standaert, ML.Pollet, RJ.Cooper, DR. Insulin-like effects of epidermal growth factor and insulin-like growth factor-I on [3H]2-deoxyglucose uptake, diacylglycerol generation and protein kinase C activation in BC3H-1 myocytes. The Biochemical journal. 261(3): 927-34, 1989.
  • Acevedo-Duncan, M.Cooper, DR.Standaert, ML.Farese, RV. Immunological evidence that insulin activates protein kinase C in BC3H-1 myocytes. FEBS letters. 244(1): 174-6, 1989.
  • Vila, MC.Cooper, DR.Davis, JS.Standaert, ML.Farese, RV. Studies of in vivo phosphorylated proteins in BC3H-1 myocytes suggest that protein kinase C is involved in insulin action. FEBS letters. 244(1): 177-80, 1989.
  • Cooper, DR.Watson, JE.Acevedo-Duncan, M.Pollet, RJ.Standaert, ML.Farese, RV. Retention of specific protein kinase C isozymes following chronic phorbol ester treatment in BC3H-1 myocytes. Biochemical and biophysical research communications. 161(1): 327-34, 1989.
  • Ishizuka, T.Hoffman, J.Cooper, DR.Watson, JE.Pushkin, DB.Farese, RV. Glucose-induced synthesis of diacylglycerol de novo is associated with translocation (activation) of protein kinase C in rat adipocytes. FEBS letters. 249(2): 234-8, 1989.
  • Ishizuka, T.Cooper, DR.Farese, RV. Insulin stimulates the translocation of protein kinase C in rat adipocytes. FEBS letters. 257(2): 337-40, 1989.
  • Farese, RV.Cooper, DR.Konda, TS.Nair, G.Standaert, ML.Davis, JS.Pollet, RJ. Mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes. The Biochemical journal. 256(1): 175-84, 1988.
  • Wallin, BA.McCafferty, JP.Fox, MJ.Cooper, DR.Goldschmidt, MS. Incidence and management of diarrhea during longterm auranofin therapy. The Journal of rheumatology. 15(12): 1755-8, 1988.
  • Farese, RV.Nair, GP.Standaert, ML.Cooper, DR. Epidermal growth factor and insulin-like growth factor I stimulate the hydrolysis of the insulin-sensitive phosphatidylinositol-glycan in BC3H-1 myocytes. Biochemical and biophysical research communications. 156(3): 1346-52, 1988.
  • Standaert, ML.Farese, RV.Cooper, DR.Pollet, RJ. Insulin-induced glycerolipid mediators and the stimulation of glucose transport in BC3H-1 myocytes. The Journal of biological chemistry. 263(18): 8696-705, 1988.
  • Nair, GP.Standaert, ML.Pollet, RJ.Cooper, DR.Farese, RV. Effects of insulin and phorbol esters on diacylglycerol generation and synthesis and hydrolysis of phosphatidylcholine in BC3H-1 myocytes. Biochemical and biophysical research communications. 154(3): 1345-9, 1988.
  • Farese, RV.Cooper, DR.Konda, TS.Nair, G.Standaert, ML.Pollet, RJ. Insulin provokes co-ordinated increases in the synthesis of phosphatidylinositol, phosphatidylinositol phosphates and the phosphatidylinositol-glycan in BC3H-1 myocytes. The Biochemical journal. 256(1): 185-8, 1988.
  • Farese, RV.Fanjul, LF.de Ruiz Galarreta, CM.Davis, JS.Cooper, DR. ACTH increases diacylglycerol content and subcellular redistribution of protein kinase C in the rat adrenal in vivo. Life sciences. 41(24): 2631-7, 1987.

  • Positions Held
  • Research Scientist/Investigator ( , James A. Haley Veterans Hospital 1986 - 2002)
  • Associate Professor (Dept. of Internal Medicine, College of Medicine, University of South Florida 1997 - 2000)
  • Associate Professor (Dept. of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1995 - 2000)
  • Assistant Professor (Dept. of Medicine, Division of Endocrinology and Medicine, College of Medicine, University of South Florida 1987 - 1997)
  • Assistant Professor (Dept. of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1992 - 1995)
  • Postdoctoral Fellow (Dept. of Medicine, James A. Haley Veterans Hospital, University of South Florida 1985 - 1986)
  • Associate Research Scientist (Biomembrane Research Program, Oklahoma Medical Research Foundation 1983 - 1985)


  • Memberships
  • American Society for Cell Biology (Member, 1997 - Present)
  • American Society for Biochemistry and Molecular Biology (Member, 1987 - Present)
  • Endocrine Society (Member, 1986 - Present)
  • American Diabetes Association (Member, 1986 - Present)
  • American Heart Association (Member, 2006 - 2006)


  • Profile last modified on 02/05/2014