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Denise Cooper, Ph.D.
Research Career Scientist,
Professor, Dept. of Molecular Medicine, College of Medicine

Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

Professor
COLLEGE OF MEDICINE MOLECULAR MEDICINE

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Contacts
Email: dcooper@health.usf.edu
Office: VAH, 2-214
Mailing address James A. Haley Veterans' Hospital 13000 Bruce B. Downs Blvd., room 2-214
Tampa, FL 33612
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Education
  • MS, Biochemistry and Molecular Biology, University of Oklahoma Health Sciences , USA - 2006.
  • PHD, Biochemistry and Molecular Biology, University Of Oklahoma Health Sciences Center, Oklahoma City, United States - 1983.
  •  
    Interdisciplinary & Emerging Signature Programs
    Metabolic Regulation and Disorders.
     


    Selected Publications
  • Sampson SR, Cooper DR. Specific protein kinase C isoforms as transducers and modulators of insulin signaling. Molecular genetics and metabolism. 89(1-2) : 32-47, .
  • Jiang K, Apostolatos AH, Ghansah T, Watson JE, Vickers T, Cooper DR, Epling-Burnette PK, Patel NA. Identification of a novel antiapoptotic human protein kinase C delta isoform, PKCdeltaVIII in NT2 cells. Biochemistry. 47(2) : 787-97, 2008.
  • Horovitz-Fried M, Jacob AI, Cooper DR, Sampson SR. Activation of the nuclear transcription factor SP-1 by insulin rapidly increases the expression of protein kinase C delta in skeletal muscle. Cellular signalling. 19(3) : 556-62, 2007.
  • Ghosh N, Patel N, Jiang K, Watson JE, Cheng J, Chalfant CE, Cooper DR. Ceramide-activated protein phosphatase involvement in insulin resistance via Akt, serine/arginine-rich protein 40, and ribonucleic acid splicing in L6 skeletal muscle cells. Endocrinology. 148(3) : 1359-66, 2007.
  • Patel NA, Song SS, Cooper DR. PKCdelta alternatively spliced isoforms modulate cellular apoptosis in retinoic acid-induced differentiation of human NT2 cells and mouse embryonic stem cells. Gene expression. 13(2) : 73-84, 2006.
  • Horovitz-Fried M, Cooper DR, Patel NA, Cipok M, Brand C, Bak A, Inbar A, Jacob AI, Sampson SR. Insulin rapidly upregulates protein kinase Cdelta gene expression in skeletal muscle. Cellular signalling. 18(2) : 183-93, 2006.
  • Patel NA, Kaneko S, Apostolatos HS, Bae SS, Watson JE, Davidowitz K, Chappell DS, Birnbaum MJ, Cheng JQ, Cooper DR. Molecular and genetic studies imply Akt-mediated signaling promotes protein kinase CbetaII alternative splicing via phosphorylation of serine/arginine-rich splicing factor SRp40. The Journal of biological chemistry. 280(14) : 14302-9, 2005.
  • Patel NA, Apostolatos HS, Mebert K, Chalfant CE, Watson JE, Pillay TS, Sparks J, Cooper DR. Insulin regulates protein kinase CbetaII alternative splicing in multiple target tissues: development of a hormonally responsive heterologous minigene. Molecular endocrinology (Baltimore, Md.). 18(4) : 899-911, 2004.
  • Patel NA, Eichler DC, Chappell DS, Illingworth PA, Chalfant CE, Yamamoto M, Dean NM, Wyatt JR, Mebert K, Watson JE, Cooper DR. The protein kinase C beta II exon confers mRNA instability in the presence of high glucose concentrations. The Journal of biological chemistry. 278(2) : 1149-57, 2003.
  • Okumura M, Yamamoto M, Sakuma H, Kojima T, Maruyama T, Jamali M, Cooper DR, Yasuda K. Leptin and high glucose stimulate cell proliferation in MCF-7 human breast cancer cells: reciprocal involvement of PKC-alpha and PPAR expression. Biochimica et biophysica acta. 1592(2) : 107-16, 2002.
  • Patel NA, Yamamoto M, Illingworth P, Mancu D, Mebert K, Chappell DS, Watson JE, Cooper DR. Phosphoinositide 3-kinase mediates protein kinase C beta II mRNA destabilization in rat A10 smooth muscle cell cultures exposed to high glucose. Archives of biochemistry and biophysics. 403(1) : 111-20, 2002.
  • Jenq W, Ramirez G, Peguero A, Cooper DR, Vesely DL. D-glucose and NaCl enhance the expression of aquaporin-1: inhibition of both by cholera toxin. Nephron. 92(2) : 279-86, 2002.
  • Patel NA, Chalfant CE, Watson JE, Wyatt JR, Dean NM, Eichler DC, Cooper DR. Insulin regulates alternative splicing of protein kinase C beta II through a phosphatidylinositol 3-kinase-dependent pathway involving the nuclear serine/arginine-rich splicing factor, SRp40, in skeletal muscle cells. The Journal of biological chemistry. 276(25) : 22648-54, 2001.
  • Yamamoto M, Acevedo-Duncan M, Chalfant CE, Patel NA, Watson JE, Cooper DR. Acute glucose-induced downregulation of PKC-betaII accelerates cultured VSMC proliferation. American journal of physiology. Cell physiology. 279(3) : C587-95, 2000.
  • Sanchez-Ramos J, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Janssen W, Patel N, Cooper DR, Sanberg PR. Adult bone marrow stromal cells differentiate into neural cells in vitro. Experimental neurology. 164(2) : 247-56, 2000.
  • Chalfant CE, Ciaraldi TP, Watson JE, Nikoulina S, Henry RR, Cooper DR. Protein kinase Ctheta expression is increased upon differentiation of human skeletal muscle cells: dysregulation in type 2 diabetic patients and a possible role for protein kinase Ctheta in insulin-stimulated glycogen synthase activity. Endocrinology. 141(8) : 2773-8, 2000.
  • Yan SF, Lu J, Zou YS, Soh-Won J, Cohen DM, Buttrick PM, Cooper DR, Steinberg SF, Mackman N, Pinsky DJ, Stern DM. Hypoxia-associated induction of early growth response-1 gene expression. The Journal of biological chemistry. 274(21) : 15030-40, 1999.
  • Patel NA, Chalfant CE, Yamamoto M, Watson JE, Eichler DC, Cooper DR. Acute hyperglycemia regulates transcription and posttranscriptional stability of PKCbetaII mRNA in vascular smooth muscle cells. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 13(1) : 103-13, 1999.
  • Jenq W, Cooper DR, Bittle P, Ramirez G. Aquaporin-1 expression in proximal tubule epithelial cells of human kidney is regulated by hyperosmolarity and contrast agents. Biochemical and biophysical research communications. 256(1) : 240-8, 1999.
  • Yamamoto M, Patel NA, Taggart J, Sridhar R, Cooper DR. A shift from normal to high glucose levels stimulates cell proliferation in drug sensitive MCF-7 human breast cancer cells but not in multidrug resistant MCF-7/ADR cells which overproduce PKC-betaII. International journal of cancer. Journal international du cancer. 83(1) : 98-106, 1999.
  • Cooper DR, Watson JE, Patel N, Illingworth P, Acevedo-Duncan M, Goodnight J, Chalfant CE, Mischak H. Ectopic expression of protein kinase CbetaII, -delta, and -epsilon, but not -betaI or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells. Archives of biochemistry and biophysics. 372(1) : 69-79, 1999.
  • Yamamoto M, Acevedo-Duncan M, Chalfant CE, Patel NA, Watson JE, Cooper DR. The roles of protein kinase C beta I and beta II in vascular smooth muscle cell proliferation. Experimental cell research. 240(2) : 349-58, 1998.
  • Chalfant CE, Watson JE, Bisnauth LD, Kang JB, Patel N, Obeid LM, Eichler DC, Cooper DR. Insulin regulates protein kinase CbetaII expression through enhanced exon inclusion in L6 skeletal muscle cells. A novel mechanism of insulin- and insulin-like growth factor-i-induced 5' splice site selection. The Journal of biological chemistry. 273(2) : 910-6, 1998.
  • Perrotti D, Bonatti S, Trotta R, Martinez R, Skorski T, Salomoni P, Grassilli E, Lozzo RV, Cooper DR, Calabretta B. TLS/FUS, a pro-oncogene involved in multiple chromosomal translocations, is a novel regulator of BCR/ABL-mediated leukemogenesis. The EMBO journal. 17(15) : 4442-55, 1998.
  • Acevedo-Duncan M, Zhang R, Cooper DR, Greenberg HM. Effects of interferon and PKC modulators on human glioma protein kinase C, cell proliferation, and cell cycle. Neurochemical research. 22(7) : 775-84, 1997.
  • Chalfant CE, Ohno S, Konno Y, Fisher AA, Bisnauth LD, Watson JE, Cooper DR. A carboxy-terminal deletion mutant of protein kinase C beta II inhibits insulin-stimulated 2-deoxyglucose uptake in L6 rat skeletal muscle cells. Molecular endocrinology (Baltimore, Md.). 10(10) : 1273-81, 1996.
  • Coulson R, Proch PS, Olsson RA, Chalfant CE, Cooper DR. Upregulated renal adenosine A1 receptors augment PKC and glucose transport but inhibit proliferation. The American journal of physiology. 270(2 Pt 2) : F263-74, 1996.
  • Jenq W, Cooper DR, Ramirez G. Integrin expression on cell adhesion function and up-regulation of P125FAK and paxillin in metastatic renal carcinoma cells. Connective tissue research. 34(3) : 161-74, 1996.
  • Yamada K, Avignon A, Standaert ML, Cooper DR, Spencer B, Farese RV. Effects of insulin on the translocation of protein kinase C-theta and other protein kinase C isoforms in rat skeletal muscles. The Biochemical journal. 308 ( Pt 1): 177-80, 1995.
  • Chalfant CE, Mischak H, Watson JE, Winkler BC, Goodnight J, Farese RV, Cooper DR. Regulation of alternative splicing of protein kinase C beta by insulin. The Journal of biological chemistry. 270(22) : 13326-32, 1995.
  • Acevedo-Duncan M, Collins J, Zhang R, Haller E, Chalfant CE, Cooper DR. In situ effects of interferon on human glioma protein kinase C-alpha and -beta ultrastructural localization. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research. 6(11) : 1353-65, 1995.
  • Zhao L, Standaert ML, Cooper DR, Avignon A, Farese RV. Effects of insulin on protein kinase-C (PKC) in HIRC-B cells: specific activation of PKC epsilon and its resistance to phorbol ester-induced down-regulation. Endocrinology. 135(6) : 2504-10, 1994.
  • Farese RV, Standaert ML, Yamada K, Huang LC, Zhang C, Cooper DR, Wang Z, Yang Y, Suzuki S, Toyota T. Insulin-induced activation of glycerol-3-phosphate acyltransferase by a chiro-inositol-containing insulin mediator is defective in adipocytes of insulin-resistant, type II diabetic, Goto-Kakizaki rats. Proceedings of the National Academy of Sciences of the United States of America. 91(23) : 11040-4, 1994.
  • Standaert ML, Musunuru K, Yamada K, Cooper DR, Farese RV. Insulin-stimulated phosphatidylcholine hydrolysis, diacylglycerol/protein kinase C signalling, and hexose transport in pertussis toxin-treated BC3H-1 myocytes. Cellular signalling. 6(6) : 707-16, 1994.
  • Gower WR, Dietz JR, Vesely DL, Finley CL, Skolnick KA, Fabri PJ, Cooper DR, Chalfant CE. Atrial natriuretic peptide gene expression in the rat gastrointestinal tract. Biochemical and biophysical research communications. 202(1) : 562-70, 1994.
  • Yamada K, Standaert ML, Yu B, Mischak H, Cooper DR, Farese RV. Insulin-like effects of sodium orthovanadate on diacylglycerol-protein kinase C signaling in BC3H-1 myocytes. Archives of biochemistry and biophysics. 312(1) : 167-72, 1994.
  • Farese RV, Standaert ML, Arnold TP, Yamada K, Musunuru K, Hernandez H, Mischak H, Cooper DR. Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes. The Journal of clinical investigation. 93(5) : 1894-9, 1994.
  • Farese RV, Standaert M, Yu B, Hernandez H, Cooper DR. 2-Hydroxypropyl-beta-cyclodextrin enhances phorbol ester effects on glucose transport and/or protein kinase C-beta translocation to the plasma membrane in rat adipocytes and soleus muscles. The Journal of biological chemistry. 268(27) : 19949-55, 1993.
  • Arnold TP, Standaert ML, Hernandez H, Watson J, Mischak H, Kazanietz MG, Zhao L, Cooper DR, Farese RV. Effects of insulin and phorbol esters on MARCKS (myristoylated alanine-rich C-kinase substrate) phosphorylation (and other parameters of protein kinase C activation) in rat adipocytes, rat soleus muscle and BC3H-1 myocytes. The Biochemical journal. 295 ( Pt 1): 155-64, 1993.
  • Cooper DR, Watson JE, Dao ML. Decreased expression of protein kinase-C alpha, beta, and epsilon in soleus muscle of Zucker obese (fa/fa) rats. Endocrinology. 133(5) : 2241-7, 1993.
  • Standaert ML, Cooper DR, Hernandez H, Arnold TP, Farese RV. Differential down-regulation of insulin-sensitive protein kinase-C isoforms by 12-O-tetradecanoylphorbol-13-acetate in rat adipocytes and BC3H-1 myocytes. Endocrinology. 132(2) : 689-92, 1993.
  • Cooper DR, Khalakdina A, Watson JE. Chronic effects of glucose on insulin signaling in A-10 vascular smooth muscle cells. Archives of biochemistry and biophysics. 302(2) : 490-8, 1993.
  • Cooper DR, Watson JE, Hernandez H, Yu B, Standaert ML, Ways DK, Arnold TT, Ishizuka T, Farese RV. Direct evidence for protein kinase C involvement in insulin-stimulated hexose uptake. Biochemical and biophysical research communications. 188(1) : 142-8, 1992.
  • Farese RV, Standaert ML, Arnold T, Yu B, Ishizuka T, Hoffman J, Vila M, Cooper DR. The role of protein kinase C in insulin action. Cellular signalling. 4(2) : 133-43, 1992.
  • Yu B, Standaert M, Arnold T, Hernandez H, Watson J, Ways K, Cooper DR, Farese RV. Effects of insulin on diacylglycerol/protein kinase-C signalling and glucose transport in rat skeletal muscles in vivo and in vitro. Endocrinology. 130(6) : 3345-55, 1992.
  • Farese RV, Standaert ML, Francois AJ, Ways K, Arnold TP, Hernandez H, Cooper DR. Effects of insulin and phorbol esters on subcellular distribution of protein kinase C isoforms in rat adipocytes. The Biochemical journal. 288 ( Pt 1): 319-23, 1992.
  • Farese RV, Standaert ML, Cooper DR. Protein kinase C downregulation? Nature. 360(6402) : 305, 1992.
  • Standaert ML, Sasse J, Cooper DR, Farese RV. Protein kinase C(19-31) pseudosubstrate inhibition of insulin action in rat adipocytes. FEBS letters. 282(1) : 139-42, 1991.
  • Farese RV, Standaert ML, Ishizuka T, Yu B, Arnold TP, Cooper DR. The role of diacylglycerol/protein kinase C signaling in insulin-stimulated glucose transport. Transactions of the Association of American Physicians. 104: 187-93, 1991.
  • Farese RV, Standaert ML, Ishizuka T, Yu B, Hernandez H, Waldron C, Watson J, Farese JP, Cooper DR, Wickstrom E. Antisense DNA downregulates protein kinase C isozymes (beta and alpha) and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes. Antisense research and development. 1(1) : 35-42, 1991.
  • Farese RV, Ishizuka T, Standaert ML, Cooper DR. Sulfonylureas activate glucose transport and protein kinase C in rat adipocytes. Metabolism: clinical and experimental. 40(2) : 196-200, 1991.
  • Coulson R, Johnson RA, Olsson RA, Cooper DR, Scheinman SJ. Adenosine stimulates phosphate and glucose transport in opossum kidney epithelial cells. The American journal of physiology. 260(6 Pt 2) : F921-8, 1991.
  • Ishizuka T, Cooper DR, Arnold T, Hernandez H, Farese RV. Downregulation of protein kinase C and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes by phorbol esters, glucose, and insulin. Diabetes. 40(10) : 1274-81, 1991.
  • Ishizuka T, Cooper DR, Hernandez H, Buckley D, Standaert M, Farese RV. Effects of insulin on diacylglycerol-protein kinase C signaling in rat diaphragm and soleus muscles and relationship to glucose transport. Diabetes. 39(2) : 181-90, 1990.
  • Cooper DR, Ishizuka T, Watson JE, Standaert ML, Nair G, Farese RV. Protein kinase C activation patterns are determined by methodological variations. Studies of insulin action in BC3H-1 myocytes and rat adipose tissue. Biochimica et biophysica acta. 1054(1) : 95-102, 1990.
  • Cooper DR, Hernandez H, Kuo JY, Farese RV. Insulin increases the synthesis of phospholipid and diacylglycerol and protein kinase C activity in rat hepatocytes. Archives of biochemistry and biophysics. 276(2) : 486-94, 1990.
  • Cooper DR, Vila MC, Watson JE, Nair G, Pollet RJ, Standaert M, Farese RV. Sulfonylurea-stimulated glucose transport association with diacylglycerollike activation of protein kinase C in BC3H1 myocytes. Diabetes. 39(11) : 1399-407, 1990.
  • Standaert ML, Buckley DJ, Ishizuka T, Hoffman JM, Cooper DR, Pollet RJ, Farese RV. Protein kinase C inhibitors block insulin and PMA-stimulated hexose transport in isolated rat adipocytes and BC3H-1 myocytes. Metabolism: clinical and experimental. 39(11) : 1170-9, 1990.
  • Ishizuka T, Cooper DR, Farese RV. Insulin provokes apparent increases in rat adipocyte M-kinase. Biochemical and biophysical research communications. 169(3) : 966-72, 1990.
  • Cooper DR, Watson JE, Acevedo-Duncan M, Pollet RJ, Standaert ML, Farese RV. Retention of specific protein kinase C isozymes following chronic phorbol ester treatment in BC3H-1 myocytes. Biochemical and biophysical research communications. 161(1) : 327-34, 1989.
  • Ishizuka T, Hoffman J, Cooper DR, Watson JE, Pushkin DB, Farese RV. Glucose-induced synthesis of diacylglycerol de novo is associated with translocation (activation) of protein kinase C in rat adipocytes. FEBS letters. 249(2) : 234-8, 1989.
  • Farese RV, Nair GP, Sierra CG, Standaert ML, Pollet RJ, Cooper DR. Insulin-like effects of epidermal growth factor and insulin-like growth factor-I on [3H]2-deoxyglucose uptake, diacylglycerol generation and protein kinase C activation in BC3H-1 myocytes. The Biochemical journal. 261(3) : 927-34, 1989.
  • Ishizuka T, Cooper DR, Farese RV. Insulin stimulates the translocation of protein kinase C in rat adipocytes. FEBS letters. 257(2) : 337-40, 1989.
  • Farese RV, Cooper DR. Potential role of phospholipid-signaling systems in insulin action and states of clinical insulin resistance. Diabetes/metabolism reviews. 5(5) : 455-74, 1989.
  • Vila MC, Cooper DR, Davis JS, Standaert ML, Farese RV. Studies of in vivo phosphorylated proteins in BC3H-1 myocytes suggest that protein kinase C is involved in insulin action. FEBS letters. 244(1) : 177-80, 1989.
  • Acevedo-Duncan M, Cooper DR, Standaert ML, Farese RV. Immunological evidence that insulin activates protein kinase C in BC3H-1 myocytes. FEBS letters. 244(1) : 174-6, 1989.
  • Wallin BA, McCafferty JP, Fox MJ, Cooper DR, Goldschmidt MS. Incidence and management of diarrhea during longterm auranofin therapy. The Journal of rheumatology. 15(12) : 1755-8, 1988.
  • Farese RV, Cooper DR, Konda TS, Nair G, Standaert ML, Davis JS, Pollet RJ. Mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes. The Biochemical journal. 256(1) : 175-84, 1988.
  • Farese RV, Nair GP, Standaert ML, Cooper DR. Epidermal growth factor and insulin-like growth factor I stimulate the hydrolysis of the insulin-sensitive phosphatidylinositol-glycan in BC3H-1 myocytes. Biochemical and biophysical research communications. 156(3) : 1346-52, 1988.
  • Farese RV, Cooper DR, Konda TS, Nair G, Standaert ML, Pollet RJ. Insulin provokes co-ordinated increases in the synthesis of phosphatidylinositol, phosphatidylinositol phosphates and the phosphatidylinositol-glycan in BC3H-1 myocytes. The Biochemical journal. 256(1) : 185-8, 1988.
  • Nair GP, Standaert ML, Pollet RJ, Cooper DR, Farese RV. Effects of insulin and phorbol esters on diacylglycerol generation and synthesis and hydrolysis of phosphatidylcholine in BC3H-1 myocytes. Biochemical and biophysical research communications. 154(3) : 1345-9, 1988.
  • Standaert ML, Farese RV, Cooper DR, Pollet RJ. Insulin-induced glycerolipid mediators and the stimulation of glucose transport in BC3H-1 myocytes. The Journal of biological chemistry. 263(18) : 8696-705, 1988.
  • Farese RV, Fanjul LF, de Ruiz Galarreta CM, Davis JS, Cooper DR. ACTH increases diacylglycerol content and subcellular redistribution of protein kinase C in the rat adrenal in vivo. Life sciences. 41(24) : 2631-7, 1987.

  • Positions Held
  • Research Scientist/Investigator ( , James A. Haley Veterans Hospital 1986 - 2002)
  • Associate Professor (Dept. of Internal Medicine, College of Medicine, University of South Florida 1997 - 2000)
  • Associate Professor (Dept. of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1995 - 2000)
  • Assistant Professor (Dept. of Medicine, Division of Endocrinology and Medicine, College of Medicine, University of South Florida 1987 - 1997)
  • Assistant Professor (Dept. of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1992 - 1995)
  • Postdoctoral Fellow (Dept. of Medicine, James A. Haley Veterans Hospital, University of South Florida 1985 - 1986)
  • Associate Research Scientist (Biomembrane Research Program, Oklahoma Medical Research Foundation 1983 - 1985)


  • Memberships
  • American Society for Cell Biology (Member, 1997 - Present)
  • American Society for Biochemistry and Molecular Biology (Member, 1987 - Present)
  • Endocrine Society (Member, 1986 - Present)
  • American Diabetes Association (Member, 1986 - Present)
  • American Heart Association (Member, 2006 - 2006)


  • Profile last modified on 02/05/2014