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Kenneth Ugen,  Ph.D.
Professor, Molecular Medicine


Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

Professor
COLLEGE OF MEDICINE MOLECULAR MEDICINE

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Contacts
Email: kugen@health.usf.edu
Phone: (813) 974-1917
Office: MDC, 4025
Mailing address Molecular Medicine COM MDC 7
Tampa, FL 33612
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Education
  • PHD, Developmental Biology, University Of Cincinnati , United States - 1986.
  • B.S., Biological Sciences, UNIVERSITY OF PITTSBURGH ,USA - 1980.
  •  
    Interdisciplinary & Emerging Signature Programs
    Cancer Biology, Allergy, Immunology & Infectious Disease, Biomedical Engineering & Nanomedicine.
     
    Research Summary

    My research focuses on applied and translational immunology with the goal of developing and characterizing novel gene based vaccines and therapies against infectious agents and cancer. Previous funded work has centered on vaccine development against neurodegenerative diseases such as Alzheimers Disease as well as the development of vaccines and immunotherapeutics against HIV. Current research emphasis is centering on methods for delivering novel gene and peptide based therapies against cancer Basic molecular and immunological methods are being used in the lab to pursue these goals

     


    Selected Publications
  • Connolly, RJ.Chapman, T.Hoff, AM.Kutzler, MA.Jaroszeski, MJ.Ugen, KE. Non-contact helium-based plasma for delivery of DNA vaccines: Enhancement of humoral and cellular immune responses. Human vaccines & immunotherapeutics. 8(11): , 2012.
  • Ugen, KE.Weiner, DB. Highlights of the DNA Vaccines 2011 Meeting. Human vaccines & immunotherapeutics. 8(11): , 2012.
  • Connolly, RJ.Rey, JI.Lambert, VM.Wegerif, G.Jaroszeski, MJ.Ugen, KE. Enhancement of antigen specific humoral immune responses after delivery of a DNA plasmid based vaccine through a contact-independent helium plasma. Vaccine. 29(39): 6781-4, 2011.
  • Marshall, WG.Boone, BA.Burgos, JD.Gografe, SI.Baldwin, MK.Danielson, ML.Larson, MJ.Caretto, DR.Cruz, Y.Ferraro, B.Heller, LC.Ugen, KE.Jaroszeski, MJ.Heller, R. Electroporation-mediated delivery of a naked DNA plasmid expressing VEGF to the porcine heart enhances protein expression. Gene therapy. 17(3): 419-23, 2010.
  • Kutzler, MA.Kraynyak, KA.Nagle, SJ.Parkinson, RM.Zharikova, D.Chattergoon, M.Maguire, H.Muthumani, K.Ugen, K.Weiner, DB. Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo. Gene therapy. 17(1): 72-82, 2010.
  • Muthumani, K.Lambert, VM.Sardesai, NY.Kim, JJ.Heller, R.Weiner, DB.Ugen, KE. Analysis of the potential for HIV-1 Vpr as an anti-cancer agent. Current HIV research. 7(2): 144-52, 2009.
  • Muthumani, K.Lambert, VM.Shanmugam, M.Thieu, KP.Choo, AY.Chung, JC.Satishchandran, A.Kim, JJ.Weiner, DB.Ugen, KE. Anti-tumor activity mediated by protein and peptide transduction of HIV viral protein R (Vpr). Cancer biology & therapy. 8(2): 180-7, 2009.
  • Fiala,M., Singer,E.J., Comminism,D., Mirzapoiazova,T., Verin,A., Espinoza,A.,Ugen,K., Bernas,M., Witte,M., Weinard,M. and Lossinsky,A.S. HIV-1 antigens in neurons of cocaine-abusing patients The Open Virology Journal. 2(): 24-31, 2008.
  • Solórzano-Vargas, RS.Vasilevko, V.Acero, G.Ugen, KE.Martinez, R.Govezensky, T.Vazquez-Ramirez, R.Kubli-Garfias, C.Cribbs, DH.Manoutcharian, K.Gevorkian, G. Epitope mapping and neuroprotective properties of a human single chain FV antibody that binds an internal epitope of amyloid-beta 1-42. Molecular immunology. 45(4): 881-6, 2008.
  • Daud, AI.DeConti, RC.Andrews, S.Urbas, P.Riker, AI.Sondak, VK.Munster, PN.Sullivan, DM.Ugen, KE.Messina, JL.Heller, R. Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 26(36): 5896-903, 2008.
  • McCray, AN.Ugen, KE.Heller, R. Enhancement of anti-melanoma activity of a plasmid expressing HIV-1 Vpr delivered through in vivo electroporation. Cancer biology & therapy. 6(8): 1269-75, 2007.
  • Li, Q.Gordon, M.Cao, C.Ugen, KE.Morgan, D. Improvement of a low pH antigen-antibody dissociation procedure for ELISA measurement of circulating anti-Abeta antibodies. BMC neuroscience. 8(): 22, 2007.
  • Ugen, KE.Nyland, SB. Injecting drugs of abuse and immunity: implications for HIV vaccine testing and efficacy. Springer seminars in immunopathology. 28(3): 281-7, 2006.
  • Kutzler, MA.Cao, C.Bai, Y.Dong, H.Choe, PY.Saulino, V.McLaughlin, L.Whelan, A.Choo, AY.Weiner, DB.Ugen, KE. Mapping of immune responses following wild-type and mutant ABeta42 plasmid or peptide vaccination in different mouse haplotypes and HLA Class II transgenic mice. Vaccine. 24(21): 4630-9, 2006.
  • Ugen, KE.Kutzler, MA.Marrero, B.Westover, J.Coppola, D.Weiner, DB.Heller, R. Regression of subcutaneous B16 melanoma tumors after intratumoral delivery of an IL-15-expressing plasmid followed by in vivo electroporation. Cancer gene therapy. 13(10): 969-74, 2006.
  • McCray, AN.Ugen, KE.Muthumani, K.Kim, JJ.Weiner, DB.Heller, R. Complete regression of established subcutaneous B16 murine melanoma tumors after delivery of an HIV-1 Vpr-expressing plasmid by in vivo electroporation. Molecular therapy : the journal of the American Society of Gene Therapy. 14(5): 647-55, 2006.
  • Nyland,S., Loughran,T.P. and Ugen,K.E. Human T Cell Lymphotropic Virus Types I and II Manual of Molecular and Clinical Laboratory Immunology. 1(): 798-802, 2006.
  • MacGregor, RR.Boyer, JD.Ugen, KE.Tebas, P.Higgins, TJ.Baine, Y.Ciccarelli, RB.Ginsberg, RS.Weiner, DB. Plasmid vaccination of stable HIV-positive subjects on antiviral treatment results in enhanced CD8 T-cell immunity and increased control of viral "blips". Vaccine. 23(17-18): 2066-73, 2005.
  • Ugen,K.E. and Wang,B. Severe acute respiratory syndrome: pathology, animal models and vaccine development DNA and Cell Biology. 24(): 477-478, 2005.
  • Kutzler, M., Ugen,K.E., and Weiner,D.B. Smallpox: Pathogenesis and Host Immune Response Relevant to Vaccine and Therapeutic Strategies for This Potential Bioterror Agent Microorganisms and Bioterrorism . (1): 63-81, 2005.
  • Heller, LC.Ugen, K.Heller, R. Electroporation for targeted gene transfer. Expert opinion on drug delivery. 2(2): 255-68, 2005.
  • Ferrantelli, F.Kitabwalla, M.Rasmussen, RA.Cao, C.Chou, TC.Katinger, H.Stiegler, G.Cavacini, LA.Bai, Y.Cotropia, J.Ugen, KE.Ruprecht, RM. Potent cross-group neutralization of primary human immunodeficiency virus isolates with monoclonal antibodies--implications for acquired immunodeficiency syndrome vaccine. The Journal of infectious diseases. 189(1): 71-4, 2004.
  • Manoutcharian, K.Acero, G.Munguia, ME.Becerril, B.Massieu, L.Govezensky, T.Ortiz, E.Marks, JD.Cao, C.Ugen, K.Gevorkian, G. Human single chain Fv antibodies and a complementarity determining region-derived peptide binding to amyloid-beta 1-42. Neurobiology of disease. 17(1): 114-21, 2004.
  • Muthumani, K.Choo, AY.Hwang, DS.Ugen, KE.Weiner, DB. HIV-1 Vpr: enhancing sensitivity of tumors to apoptosis. Current drug delivery. 1(4): 335-44, 2004.
  • Wilcock, DM.Munireddy, SK.Rosenthal, A.Ugen, KE.Gordon, MN.Morgan, D. Microglial activation facilitates Abeta plaque removal following intracranial anti-Abeta antibody administration. Neurobiology of disease. 15(1): 11-20, 2004.
  • Giri, M.Ugen, KE.Weiner, DB. DNA vaccines against human immunodeficiency virus type 1 in the past decade. Clinical microbiology reviews. 17(2): 370-89, 2004.
  • Cao, C.Bai, Y.Holloway, MJ.Edgeworth, RL.Jackson, EA.Cotropia, J.Ugen, KE. Characterization of a novel human anti-HIV-1 gp41 IgM monoclonal antibody designated clone 37. DNA and cell biology. 23(12): 836-41, 2004.
  • Li, Q.Cao, C.Chackerian, B.Schiller, J.Gordon, M.Ugen, KE.Morgan, D. Overcoming antigen masking of anti-amyloidbeta antibodies reveals breaking of B cell tolerance by virus-like particles in amyloidbeta immunized amyloid precursor protein transgenic mice. BMC neuroscience. 5(): 21, 2004.
  • Manoutcharian, K.Acero, G.Munguia, ME.Montero, JA.Govezensky, T.Cao, C.Ugen, K.Gevorkian, G. Amyloid-beta peptide-specific single chain Fv antibodies isolated from an immune phage display library. Journal of neuroimmunology. 145(1-2): 12-7, 2003.
  • Ugen, KE.Heller, R. Electroporation as a method for the efficient in vivo delivery of therapeutic genes. DNA and cell biology. 22(12): 753, 2003.
  • CAo,C., Ugen,K.E., Dickey,C., Wilcock,D, Bai,Y., Gordon,M.N. and Morgan,D. Characterization of amyloid beta vaccination strategies in mice Fondation IPSEN Pour La Recherche Therapeutique. 1(): 21-31, 2003.
  • Morgan,D., Jantzen,P., Wilcock,D., DiCarlo,G., Ugen,K. and Gordon,M. The association of microglial activation and amyloid resuction in APP+PS1 transgenic mice Current Medicinal Chemistry, Immunology, Endocrine and Metabolic Agents. 3(): 233-243, 2003.
  • Wilcock, DM.DiCarlo, G.Henderson, D.Jackson, J.Clarke, K.Ugen, KE.Gordon, MN.Morgan, D. Intracranially administered anti-Abeta antibodies reduce beta-amyloid deposition by mechanisms both independent of and associated with microglial activation. The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(9): 3745-51, 2003.
  • Austin, L.Arendash, GW.Gordon, MN.Diamond, DM.DiCarlo, G.Dickey, C.Ugen, K.Morgan, D. Short-term beta-amyloid vaccinations do not improve cognitive performance in cognitively impaired APP + PS1 mice. Behavioral neuroscience. 117(3): 478-84, 2003.
  • Muthumani, K.Bagarazzi, M.Conway, D.Hwang, DS.Manson, K.Ciccarelli, R.Israel, Z.Montefiori, DC.Ugen, K.Miller, N.Kim, J.Boyer, J.Weiner, DB. A Gag-Pol/Env-Rev SIV239 DNA vaccine improves CD4 counts, and reduce viral loads after pathogenic intrarectal SIV(mac)251 challenge in rhesus Macaques. Vaccine. 21(7-8): 629-37, 2003.
  • Nyland, SB.Cao, C.Bai, Y.Loughran, TP.Ugen, KE. Modulation of infection and type 1 cytokine expression parameters by morphine during in vitro coinfection with human T-cell leukemia virus type I and HIV-1. Journal of acquired immune deficiency syndromes (1999). 32(4): 406-16, 2003.
  • Nyland,S.B., Cao,C. and Ugen,K.E. Human T Cell Lymphotropic Virus Type I and II Manual of Clinical Laboratory Immunology. 1(): 749-753, 2002.
  • Edgeworth, RL.San, JH.Rosenzweig, JA.Nguyen, NL.Boyer, JD.Ugen, KE. Vaccine development against HIV-1: current perspectives and future directions. Immunologic research. 25(1): 53-74, 2002.
  • Gómez-Román, VR.Cao, C.Bai, Y.Santamaría, H.Acero, G.Manoutcharian, K.Weiner, DB.Ugen, KE.Gevorkian, G. Phage-displayed mimotopes recognizing a biologically active anti-HIV-1 gp120 murine monoclonal antibody. Journal of acquired immune deficiency syndromes (1999). 31(2): 147-53, 2002.
  • MacGregor, RR.Ginsberg, R.Ugen, KE.Baine, Y.Kang, CU.Tu, XM.Higgins, T.Weiner, DB.Boyer, JD. T-cell responses induced in normal volunteers immunized with a DNA-based vaccine containing HIV-1 env and rev. AIDS (London, England). 16(16): 2137-43, 2002.
  • Wilcock, DM.Gordon, MN.Ugen, KE.Gottschall, PE.DiCarlo, G.Dickey, C.Boyett, KW.Jantzen, PT.Connor, KE.Melachrino, J.Hardy, J.Morgan, D. Number of Abeta inoculations in APP+PS1 transgenic mice influences antibody titers, microglial activation, and congophilic plaque levels. DNA and cell biology. 20(11): 731-6, 2001.
  • Dickey, CA.Morgan, DG.Kudchodkar, S.Weiner, DB.Bai, Y.Cao, C.Gordon, MN.Ugen, KE. Duration and specificity of humoral immune responses in mice vaccinated with the Alzheimer's disease-associated beta-amyloid 1-42 peptide. DNA and cell biology. 20(11): 723-9, 2001.
  • Kim, JJ.Yang, JS.Nottingham, LK.Lee, DJ.Lee, M.Manson, KH.Wyand, MS.Boyer, JD.Ugen, KE.Weiner, DB. Protection from immunodeficiency virus challenges in rhesus macaques by multicomponent DNA immunization. Virology. 285(2): 204-17, 2001.
  • Ugen, KE.Morgan, D. Alzheimer's disease: molecularly based immunotherapeutics. DNA and cell biology. 20(11): 677-8, 2001.
  • Han, TK.Yoder, S.Cao, C.Ugen, KE.Dao, ML. Expression of Streptococcus mutans wall-associated protein A gene in Chinese hamster ovary cells: prospect for a dental caries DNA vaccine. DNA and cell biology. 20(9): 595-601, 2001.
  • Nyland, SB.Specter, S.Ugen, KE. Direct vs. indirect modulation of complex in vitro human retroviral infections by morphine. Advances in experimental medicine and biology. 493(): 49-58, 2001.
  • Sin, JI.Kim, J.Chattergoon, M.Ayyavoo, V.McCallus, D.Ugen, KE.Boyer, JD.Weiner, DB. Engineering of DNA vaccines using molecular adjuvant plasmids. Developments in biologicals. 104(): 187-98, 2000.
  • Dickey, C.Ziegner, U.Agadjanyan, MG.Srikantan, V.Refaeli, Y.Prabhu, A.Sato, A.Williams, WV.Weiner, DB.Ugen, KE. Murine monoclonal antibodies biologically active against the amino region of HIV-1 gp120: isolation and characterization. DNA and cell biology. 19(4): 243-52, 2000.
  • Morgan, D.Diamond, DM.Gottschall, PE.Ugen, KE.Dickey, C.Hardy, J.Duff, K.Jantzen, P.DiCarlo, G.Wilcock, D.Connor, K.Hatcher, J.Hope, C.Gordon, M.Arendash, GW. A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease. Nature. 408(6815): 982-5, 2000.
  • Kim, JJ.Nottingham, LK.Tsai, A.Lee, DJ.Maguire, HC.Oh, J.Dentchev, T.Manson, KH.Wyand, MS.Agadjanyan, MG.Ugen, KE.Weiner, DB. Antigen-specific humoral and cellular immune responses can be modulated in rhesus macaques through the use of IFN-gamma, IL-12, or IL-18 gene adjuvants. Journal of medical primatology. 28(4-5): 214-23, 2000.
  • Edgeworth, RL.Ugen, KE. Immunopathological factors for vertical transmission of HIV-1. Pathobiology : journal of immunopathology, molecular and cellular biology. 68(2): 53-67, 2000.
  • Cotropia,J.P. and Ugen,K.E. Passive Immunotherapy Against HIV-1: Current Status and Potential Human Retroviral Infections: Immunotherpeutic and Therapeutic Control. 1(): 217-238, 2000.
  • Kim, JJ.Yang, JS.VanCott, TC.Lee, DJ.Manson, KH.Wyand, MS.Boyer, JD.Ugen, KE.Weiner, DB. Modulation of antigen-specific humoral responses in rhesus macaques by using cytokine cDNAs as DNA vaccine adjuvants. Journal of virology. 74(7): 3427-9, 2000.
  • Boyer, JD.Cohen, AD.Ugen, KE.Edgeworth, RL.Bennett, M.Shah, A.Schumann, K.Nath, B.Javadian, A.Bagarazzi, ML.Kim, J.Weiner, DB. Therapeutic immunization of HIV-infected chimpanzees using HIV-1 plasmid antigens and interleukin-12 expressing plasmids. AIDS (London, England). 14(11): 1515-22, 2000.
  • Yoder, S.Cao, C.Ugen, KE.Dao, ML. High-level expression of a truncated wall-associated protein A from the dental cariogenic Streptococcus mutans. DNA and cell biology. 19(7): 401-8, 2000.
  • Viveros, M.Dickey, C.Cotropia, JP.Gevorkian, G.Larralde, C.Broliden, K.Levi, M.Burgess, A.Cao, C.Weiner, DB.Agadjanyan, MG.Ugen, KE. Characterization of a novel human immunodeficiency virus type 1 neutralizable epitope within the immunodominant region of gp41. Virology. 270(1): 135-45, 2000.
  • Boyer, JD.Chattergoon, MA.Ugen, KE.Shah, A.Bennett, M.Cohen, A.Nyland, S.Lacy, KE.Bagarazzi, ML.Higgins, TJ.Baine, Y.Ciccarelli, RB.Ginsberg, RS.MacGregor, RR.Weiner, DB. Enhancement of cellular immune response in HIV-1 seropositive individuals: A DNA-based trial. Clinical immunology (Orlando, Fla.). 90(1): 100-7, 1999.
  • Nyland, SB.Specter, S.Ugen, KE. Morphine effects on HTLV-I infection in the presence or absence of concurrent HIV-1 infection. DNA and cell biology. 18(4): 285-91, 1999.
  • Kang, LC.Kerben, MJ.Ugen, KE.Specter, SC. Modulation of ex vivo cytokine production by splenocytes using in vitro combined therapeutics in murine retroviral model. DNA and cell biology. 18(7): 585-92, 1999.
  • Bagarazzi, ML.Boyer, JD.Javadian, MA.Chattergoon, MA.Shah, AR.Cohen, AD.Bennett, MK.Ciccarelli, RB.Ugen, KE.Weiner, DB. Systemic and mucosal immunity is elicited after both intramuscular and intravaginal delivery of human immunodeficiency virus type 1 DNA plasmid vaccines to pregnant chimpanzees. The Journal of infectious diseases. 180(4): 1351-5, 1999.
  • Boyer, JD.Kim, J.Ugen, K.Cohen, AD.Ahn, L.Schumann, K.Lacy, K.Bagarazzi, ML.Javadian, A.Ciccarelli, RB.Ginsberg, RS.MacGregor, RR.Weiner, DB. HIV-1 DNA vaccines and chemokines. Vaccine. 17 Suppl 2(): S53-64, 1999.
  • Ugen, KE.Nyland, SB.Boyer, JD.Vidal, C.Lera, L.Rasheid, S.Chattergoon, M.Bagarazzi, ML.Ciccarelli, R.Higgins, T.Baine, Y.Ginsberg, R.Macgregor, RR.Weiner, DB. DNA vaccination with HIV-1 expressing constructs elicits immune responses in humans. Vaccine. 16(19): 1818-21, 1998.
  • Noe, SN.Nyland, SB.Ugen, K.Friedman, H.Klein, TW. Cannabinoid receptor agonists enhance syncytia formation in MT-2 cells infected with cell free HIV-1MN. Advances in experimental medicine and biology. 437(): 223-9, 1998.
  • MacGregor, RR.Boyer, JD.Ugen, KE.Lacy, KE.Gluckman, SJ.Bagarazzi, ML.Chattergoon, MA.Baine, Y.Higgins, TJ.Ciccarelli, RB.Coney, LR.Ginsberg, RS.Weiner, DB. First human trial of a DNA-based vaccine for treatment of human immunodeficiency virus type 1 infection: safety and host response. The Journal of infectious diseases. 178(1): 92-100, 1998.
  • Bagarazzi, ML.Boyer, JD.Ugen, KE.Javadian, MA.Chattergoon, M.Shah, A.Bennett, M.Ciccarelli, R.Carrano, R.Coney, L.Weiner, DB. Safety and immunogenicity of HIV-1 DNA constructs in chimpanzees. Vaccine. 16(19): 1836-41, 1998.
  • Boyer, J.Ugen, K.Wang, B.Chattergoon, M.Tsai, A.Merva, M.Weiner, DB. Induction of a TH1 type cellular immune response to the human immunodeficiency type 1 virus by in vivo DNA inoculation. Developments in biological standardization. 92(): 169-74, 1998.
  • Nyland, SB.Specter, S.Im-Sin, J.Ugen, KE. Opiate effects on in vitro human retroviral infection. Advances in experimental medicine and biology. 437(): 91-100, 1998.
  • Sin, JI.Kim, JJ.Ugen, KE.Ciccarelli, RB.Higgins, TJ.Weiner, DB. Enhancement of protective humoral (Th2) and cell-mediated (Th1) immune responses against herpes simplex virus-2 through co-delivery of granulocyte-macrophage colony-stimulating factor expression cassettes. European journal of immunology. 28(11): 3530-40, 1998.
  • Ugen, K.E., Rushing,G., Vidal, C., Kim,J.,, Javadian,A., Boyer,J. and Weiner, D.B. Beta chemokine levels in non-human primates modulated in vivo by HIv-1 DNA vaccines World AIDS Conference Proceedings. 5(): 55-59, 1998.
  • Kim,J.J., Nottingham,L.K., Tsai,A., Morrison,L.D., Oh,J., Dentchev,T., VanCott,T.C., Manson, K.H., Ugen,K.E. and Weiner,D.B. Modulation of protective immunity in macaques using molecular adjuvants World AIDS Conference Proceedings. 5(): 35-39, 1998.
  • Boyer,J.D., Bennett,M., Chattergoon,M.A., Ugen,K.E., Shah,A., Cohen,A., Bagarazzi,M.L., Higgins,T.J., Baine,Y., Ciccarelli,R.B., Ginsberg,R.S., Kim,J.J., MacGregor,R.R. and Weiner, D.B. Enhancement of cellular immune response in patients seropositive for HIV-1 utilizing DNA vaccines World AIDS Conference Proceedings. 2(): 441-446, 1998.
  • Agadjanyan, MG.Wang, B.Nyland, SB.Weiner, DB.Ugen, KE. DNA plasmid based vaccination against the oncogenic human T cell leukemia virus type 1. Current topics in microbiology and immunology. 226(): 175-92, 1998.
  • Agadjanyan, MG.Chattergoon, MA.Petrushina, I.Bennett, M.Kim, J.Ugen, KE.Kieber-Emmons, T.Weiner, DB. Monoclonal antibodies define a cellular antigen involved in HTLV-I infection. Hybridoma. 17(1): 9-19, 1998.
  • Ugen,K.E., Boyer,J., Wang,B.,, Gluckman,S., Williams, W.V., Nyland,S., Carmack,B., Agadjanyan, M.G., Bagarazzi,M, Higgins,T., Coney,L., Ciccarelli,R.,, Ginsberg,R, MacGregor,R.R. and Weiner, D.B. Facilitated nucleic acid vaccination of humans with an HIV-1 expressing DNA boosts immune responses against the envelope glycoprotein Vaccines . 97(1): 145-149, 1997.
  • Boyer,J.D., Ugen,K., Chattergoon,M., Tsai,A, Coney, L. and Weiner,D.B Induction of a cross reactive cellular immune response to HIV-1 by in vivo DNA inoculation Vaccines. 97(1): 137-140, 1997.
  • Mahalingam, S.MacDonald, B.Ugen, KE.Ayyavoo, V.Agadjanyan, MG.Williams, WV.Weiner, DB. In vitro and in vivo tumor growth suppression by HIV-1 Vpr. DNA and cell biology. 16(2): 137-43, 1997.
  • Agadjanyan,M.G., Trivedi,N., Kudchodkar,S., Bennett,M., Ugen,K.E. and Weiner,D.B. HIV-2 expression vector generates cross strain humoral and cellular anti-HIV-2 as well as anti-SIV immune responses Vaccines. 97(1): , 1997.
  • Ugen, KE.Srikantan, V.Goedert, JJ.Nelson, RP.Williams, WV.Weiner, DB. Vertical transmission of human immunodeficiency virus type 1: seroreactivity by maternal antibodies to the carboxy region of the gp41 envelope glycoprotein. The Journal of infectious diseases. 175(1): 63-9, 1997.
  • Weiner,D.B., Ugen,K.E. and Williams,W.V. Methods and compositions for diagnosing and treating certain HIV infected patients Biotechnology Advances. 15(): 438, 1997.
  • Wang, B.Dang, K.Agadjanyan, MG.Srikantan, V.Li, F.Ugen, KE.Boyer, J.Merva, M.Williams, WV.Weiner, DB. Mucosal immunization with a DNA vaccine induces immune responses against HIV-1 at a mucosal site. Vaccine. 15(8): 821-5, 1997.
  • Boyer, JD.Ugen, KE.Wang, B.Agadjanyan, M.Gilbert, L.Bagarazzi, ML.Chattergoon, M.Frost, P.Javadian, A.Williams, WV.Refaeli, Y.Ciccarelli, RB.McCallus, D.Coney, L.Weiner, DB. Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination. Nature medicine. 3(5): 526-32, 1997.
  • Boyer, JD.Ugen, KE.Chattergoon, M.Wang, B.Shah, A.Agadjanyan, M.Bagarazzi, ML.Javadian, A.Carrano, R.Coney, L.Williams, WV.Weiner, DB. DNA vaccination as anti-human immunodeficiency virus immunotherapy in infected chimpanzees. The Journal of infectious diseases. 176(6): 1501-9, 1997.
  • Agadjanyan, MG.Trivedi, NN.Kudchodkar, S.Bennett, M.Levine, W.Lin, A.Boyer, J.Levy, D.Ugen, KE.Kim, JJ.Weiner, DB. An HIV type 2 DNA vaccine induces cross-reactive immune responses against HIV type 2 and SIV. AIDS research and human retroviruses. 13(18): 1561-72, 1997.
  • Ugen, KE.Boyer, JD.Wang, B.Bagarazzi, M.Javadian, A.Frost, P.Merva, MM.Agadjanyan, MG.Nyland, S.Williams, WV.Coney, L.Ciccarelli, R.Weiner, DB. Nucleic acid immunization of chimpanzees as a prophylactic/immunotherapeutic vaccination model for HIV-1: prelude to a clinical trial. Vaccine. 15(8): 927-30, 1997.
  • Boyer,J.D., Wang, B., Ugen,K.E., Srikantan,V., Gilbert,L., Kudchodkar,S., Javadian.A., Dang,K., Merva,M., McCallus, D., Ciccarelli,R., Carrano,R.A., Coney,L. and Weiner, D. B. Induction of humoral and cellular immune responses to HIV-1 in chimpanzees by in vivo DNA inoculation Vaccines. 96(1): 51-54, 1996.
  • McCallus,D., Coney,L., Wang,B., Boyer,J., Pachuk,C., Higgins,T., Carrano,R., Sims,D., Ugen,K., Weiner,D.B. and Ciccarelli, R Facilitated DNA injection elicits specific anti-HIV immune responses in preclinical animal models Vaccines. 96(1): 55-60, 1996.
  • Ugen,K.E., Boyer,J.D., Agadjanyan,M.G., BAng,B., Javadian,A., Coney,L, McCallus,D. Williams, W.V., Ciccarelli,R., Carrano,R. and Weiner,D.B. Site-specific humoral immune responses generated in chimpanzees after DNA inoculation with HIV-1 envelope expressing constructs Vaccines. 96(1): 61-65, 1996.
  • Ugen,K.E., Fernandes,L., Wang,B, Weiner,D.B. and Williams, W.V. Retroviruses and Autoimmunity Microrganisms and Autoimmune Diseases. (): 219-231, 1996.
  • Ayyavoo, V.Ugen, KE.Fernandes, LS.Goedert, JJ.Rubinstein, A.Williams, WV.Weiner, DB. Analysis of genetic heterogeneity, antigenicity, and biological characteristics of HIV-1 in a maternal transmitter and nontransmitter patient pair. DNA and cell biology. 15(7): 571-80, 1996.
  • Boyer, JD.Wang, B.Ugen, KE.Agadjanyan, M.Javadian, A.Frost, P.Dang, K.Carrano, RA.Ciccarelli, R.Coney, L.Williams, WV.Weiner, DB. In vivo protective anti-HIV immune responses in non-human primates through DNA immunization. Journal of medical primatology. 25(3): 242-50, 1996.
  • Cotropia, J.Ugen, KE.Kliks, S.Broliden, K.Broliden, PA.Hoxie, JA.Srikantan, V.Williams, WV.Weiner, DB. A human monoclonal antibody to HIV-1 gp41 with neutralizing activity against diverse laboratory isolates. Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association. 12(3): 221-32, 1996.
  • Ugen,K.E., Agadjanyan,M.G., Wang,B., Kudchodkar, S.,, Merva,M., Boyer,J.D., Li,F., Williams, W.V. and Weiner, D.B. Elicitation of immune responses on rodents after DNA inoculation with an HTLV-I envelope glycoprotein expressing construct: a model for vaccination efficacy Vaccines. 95(1): 129-134, 1995.
  • Boyer,J.D., Wang,B., Ugen,K.E., Agadjanyan, M.G., Wang, B., Kudchodkar,S., Merva, M., Boyer, J.D., Li,F., Willimas, W.V. and Weiner, D.B. Induction of humoral and cellular immune response to HIV-1 in Cynomologous macaques and chimpanzees Vaccines. 95(1): 99-104, 1995.
  • Ugen, KE. Vertical transmission of HIV-1 infection. Diagnosis and prevention. The Journal of the Florida Medical Association. 82(12): 825-6, 1995.
  • Wang, B.Boyer, JD.Ugen, KE.Srikantan, V.Ayyaroo, V.Agadjanyan, MG.Williams, WV.Newman, M.Coney, L.Carrano, R. Nucleic acid-based immunization against HIV-I: induction of protective in vivo immune responses. AIDS . 9 Suppl A(): S159-70, 1995.
  • Wang, B.Merva, M.Dang, K.Ugen, KE.Williams, WV.Weiner, DB. Immunization by direct DNA inoculation induces rejection of tumor cell challenge. Human gene therapy. 6(4): 407-18, 1995.
  • Wang, B.Boyer, J.Srikantan, V.Ugen, K.Agadjanian, M.Merva, M.Gilbert, L.Dang, K.McCallus, D.Moelling, K. DNA inoculation induces cross clade anti-HIV-1 responses. Annals of the New York Academy of Sciences. 772(): 186-97, 1995.
  • Wang, B.Boyer, J.Srikantan, V.Ugen, K.Gilbert, L.Phan, C.Dang, K.Merva, M.Agadjanyan, MG.Newman, M. Induction of humoral and cellular immune responses to the human immunodeficiency type 1 virus in nonhuman primates by in vivo DNA inoculation. Virology. 211(1): 102-12, 1995.
  • Ugen, KE.Wang, B.Ayyavoo, V.Agadjanyan, M.Boyer, J.Li, F.Kudchodkar, S.Lin, J.Merva, M.Fernandes, L. DNA inoculation as a novel vaccination method against human retroviruses with rheumatic disease associations. Immunologic research. 13(2-3): 154-62, 1994.
  • Srikantan, V.Wang, B.Satre, MA.Ugen, KE.Dang, K.Scales, F.Godillot, AP.Williams, WV.Weiner, DB. Cloning and biological characterization of human single-chain Fv fragments that mediate neutralization of HIV-1. AIDS (London, England). 8(11): 1525-32, 1994.
  • Prammer, KV.Boyer, J.Ugen, K.Shattil, SJ.Kieber-Emmons, T. Bioactive Arg-Gly-Asp conformations in anti-integrin GPIIb-IIIa antibodies. Receptor. 4(2): 93-108, 1994.
  • Ugen, KE.Von Feldt, JM.Weiner, DB.Ziegner, UH. Diagnosis and prediction of pediatric HIV-1 infection and AIDS: current status. Journal of clinical laboratory analysis. 8(5): 309-314, 1994.
  • Wang, B.Merva, M.Dang, K.Ugen, KE.Boyer, J.Williams, WV.Weiner, DB. DNA inoculation induces protective in vivo immune responses against cellular challenge with HIV-1 antigen-expressing cells. AIDS research and human retroviruses. 10 Suppl 2(): S35-41, 1994.
  • Williams, WV.VonFeldt, JM.Rosenbaum, H.Ugen, KE.Weiner, DB. Molecular cloning of a soluble form of the granulocyte-macrophage colony-stimulating factor receptor alpha chain from a myelomonocytic cell line. Expression, biologic activity, and preliminary analysis of transcript distribution. Arthritis and rheumatism. 37(10): 1468-78, 1994.
  • Agadjanyan, MG.Ugen, KE.Wang, B.Williams, WV.Weiner, DB. Identification of an 80-kilodalton membrane glycoprotein important for human T-cell leukemia virus type I and type II syncytium formation and infection. Journal of virology. 68(1): 485-93, 1994.
  • Coney, L.Wang, B.Ugen, KE.Boyer, J.McCallus, D.Srikantan, V.Agadjanyan, M.Pachuk, CJ.Herold, K.Merva, M. Facilitated DNA inoculation induces anti-HIV-1 immunity in vivo. Vaccine. 12(16): 1545-50, 1994.
  • wang,B, Merva,M., Dang,K., Boyer,J., Ugen,K.E., LiVolsi,V.,, Wilson,D., Li,F., Schumacher,H.R.,, Williams,W.V. and Weiner,D.B. A novel system to evaluate DNA inocualtion techniques for induction of in vivo protection Vaccines. 94(1): 83-90, 1994.
  • Sato, AI.Balamuth, FB.Ugen, KE.Williams, WV.Weiner, DB. Identification of CD7 glycoprotein as an accessory molecule in HIV-1-mediated syncytium formation and cellfree infection. Journal of immunology (Baltimore, Md. : 1950). 152(10): 5142-52, 1994.
  • Ugen,K.E., Galetta,S.M., VonFeldt,J.M., McCallus,D.E., Williams,W.V. and Weiner,D.B. Ocular manifestations of HIV-1 infection The AIDS Reader. 4(): 66-71, 1994.
  • Goedert,J.J., Robert-Guroff,M., Ugen,K., Weiner,D.B., Matthews,T., Drummond,J., Rubinstein,A., Rossi,P., Durda,P., Zwart, G., Goudsmit,J., Allain,J.P., Walsh,J., Blattner,W., Mathison,B. and Dublin,S. Perinatal transmission of HIV type 1: associations with maternal and anti-HIV serological reactivity AIDS Res. Hum. Retro. 10(): 1125-1134, 1994.
  • Ugen,K.E.,Srikantan,V., Wang,B.,Goedert,J., Ziegner,U., Agadjanyan,M.G., Boyer,J.D., Cotropia,J.P., Williams,W.V. and Weiner,D.B. Vertical transmission of human retroviral infections: immunological parameters Arch of STD/HIV Research. 8(): 285-292, 1994.
  • Soprano, DR.Gyda, M.Jiang, H.Harnish, DC.Ugen, K.Satre, M.Chen, L.Soprano, KJ.Kochhar, DM. A sustained elevation in retinoic acid receptor-beta 2 mRNA and protein occurs during retinoic acid-induced fetal dysmorphogenesis. Mechanisms of development. 45(3): 243-53, 1994.
  • Eaton, AM.Ugen, KE.Weiner, DB.Wildes, T.Levy, JA. An anti-gp41 human monoclonal antibody that enhances HIV-1 infection in the absence of complement. AIDS research and human retroviruses. 10(1): 13-8, 1994.
  • D'Angelo, M.Chen, JM.Ugen, K.Greene, RM. TGF beta 1 regulation of collagen metabolism by embryonic palate mesenchymal cells. The Journal of experimental zoology. 270(2): 189-201, 1994.
  • Wang,B., Ugen,K.E., Srikantan,V., Agadjanyan,V., Dang,K., Sato,A.I., Refaeli,Y., Boyer,J., Williams,W.V. and Weiner,D.B. Gene immunization: a novel method for vaccine development against HIV Vaccines. 93(1): 143-150, 1993.
  • Ugen,K.E., Refaeli,Y., Ziegner,U., Agadjanyan,M.G., Satre,M., Srikantan,V., Wang,B., Sato,A., Williams,W.V. and Weiner,D.B. Generation of monoclonal antibodies against the amino region of gp120 that elicit antibody dependent cellular cytotoxicity Vaccines. 93(1): 215-221, 1993.
  • Wang, B.Ugen, KE.Srikantan, V.Agadjanyan, MG.Dang, K.Refaeli, Y.Sato, AI.Boyer, J.Williams, WV.Weiner, DB. Gene inoculation generates immune responses against human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences of the United States of America. 90(9): 4156-4160, 1993.
  • Bhat, S.Mettus, RV.Reddy, EP.Ugen, KE.Srikanthan, V.Williams, WV.Weiner, DB. The galactosyl ceramide/sulfatide receptor binding region of HIV-1 gp120 maps to amino acids 206-275. AIDS research and human retroviruses. 9(2): 175-81, 1993.
  • Wang, B.Boyer, J.Srikantan, V.Coney, L.Carrano, R.Phan, C.Merva, M.Dang, K.Agadjanan, M.Gilbert, L. DNA inoculation induces neutralizing immune responses against human immunodeficiency virus type 1 in mice and nonhuman primates. DNA and cell biology. 12(9): 799-805, 1993.
  • Wang, B.Agadjanyan, MG.Srikantan, V.Ugen, KE.Hall, W.Kaplan, MH.Dang, K.Williams, WV.Weiner, DB. Molecular cloning, expression, and biological characterization of an HTLV-II envelope glycoprotein: HIV-1 expression is permissive for HTLV-II-induced cell fusion. AIDS research and human retroviruses. 9(9): 849-60, 1993.
  • Agadjanyan,M.G.,, Ugen,K.E., Wang,B., Willimas,W.V. and Weiner,D.B. DNA inoculation with an HTLV-I envelope DNA construct elicits immune responses in rabbits Vaccines. 94(1): 47-53, 1993.
  • Ugen,K.E., Goedert,J.J. and Weiner,D.B. Maternal antibodies against HIV-1 envelope glycoprotein: model to evaluate vertical transmission/potential vaccine efficacy Arch of STD/HIV Research. 7(): 223-228, 1993.
  • VonFeldt,J.M., Ugen,K.E.,Weiner,D.B. and Williams, W.V. Bioactive peptides based on antibody structure Biomedical applications of biotechnology. 92(1): 55-85, 1992.
  • Ugen,K.E., Goedert,J.J.,Boyer,J., Refaeli,Y.,Williams,W.V.,Willoughby,A., Landesman,S., Rubinstein,A, Kieber-Emmons,T., Berzofsky,J. and Weiner,D.B. Vertical transmission of HIV-1 infection:maternal humoral immune responses to gp120 and gp41 peptides Vaccines. 92(1): 183-189, 1992.
  • Sato,A.I., Ugen,K.E., Williams,W.V., Perussia,B. and Weiner,D.B. Identification of two non-CD4 molecules important for HIV-1 mediated syncytium formation Vaccines. 92(1): 223-228, 1992.
  • Von Feldt, JM.Kieber-Emmons, T.Weiner, DB.Ugen, KE.Williams, WV. Molecular structure and granulocyte/macrophage colony-stimulating factor activity. DNA and Cell Biology. 11(3): 183-91, 1992.
  • Kieber-Emmons, T.Krowka, JF.Boyer, J.Ugen, KE.Williams, WV.Morrow, WJ.Weiner, DB. Immunological characteristics of the putative CD4-binding site of the HIV-1 envelope protein. Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology. 60(4): 187-194, 1992.
  • Laczkó, I.Hollósi, M.Urge, L.Ugen, KE.Weiner, DB.Mantsch, HH.Thurin, J.Otvös, L. Synthesis and conformational studies of N-glycosylated analogues of the HIV-1 principal neutralizing determinant. Biochemistry. 31(17): 4282-4288, 1992.
  • Ugen, KE.McCallus, DE.Von Feldt, JM.Williams, WV.Greene, MI.Weiner, DB. Ocular tissue involvement in HIV infection: immunological and pathological aspects. Immunologic Research. 11(2): 141-153, 1992.
  • Ugen, KE.Goedert, JJ.Boyer, J.Refaeli, Y.Frank, I.Williams, WV.Willoughby, A.Landesman, S.Mendez, H.Rubinstein, A. Vertical transmission of human immunodeficiency virus (HIV) infection. Reactivity of maternal sera with glycoprotein 120 and 41 peptides from HIV type 1. The Journal of Clinical Investigation. 89(6): 1923-1930, 1992.
  • Cotropia,J.P., Ugen,K.E., Lambert,D., Ljunggren-Broliden,K., Kliks,S., Hoxie,J. and Weiner,D.B. Characterization of human monoclonal antibodies (HuMab) to the HiV-1 transmembrane (TM) gp41 protein Vaccines. 92(1): 157-163, 1992.
  • Satre, MA.Ugen, KE.Kochhar, DM. Developmental changes in endogenous retinoids during pregnancy and embryogenesis in the mouse. Biology of reproduction. 46(5): 802-10, 1992.
  • Osther, K.Wiik, A.Black, F.Skinhøj, P.Kellermann, G.Ugen, KE.Williams, WV.Weiner, DB. PASSHIV-1 treatment of patients with HIV-1 infection. A preliminary report of a phase I trial of hyperimmune porcine immunoglobulin to HIV-1. AIDS . 6(12): 1457-1464, 1992.
  • McCallus, DE.Ugen, KE.Sato, AI.Williams, WV.Weiner, DB. Construction of a recombinant bacterial human CD4 expression system producing a bioactive CD4 molecule. Viral Immunology. 5(2): 163-172, 1992.
  • Ugen,K.E., Perussia,B., Kamoun,M., Merva,M.J., Williams,W.V.,Nara,P.,Kieber-Emmons,T. and Weiner,D.B. Inhibition of HIV-1 cellular infection by immunologic reagents Vaccines. 91(1): 115-121, 1991.
  • Kieber-Emmons,T., Ugen,K.E., Merva,M.J., Whalley,A., Morrow,W.J. Engineered peptides that mimic HIV-1 neutraliziing envelope structures Vaccines. 91(1): 165-173, 1991.
  • Osther, K.Kellermann, G.Ugen, KE.Williams, WV.Weiner, DB. Protective humoral immune responses to the human immunodeficiency virus induced in immunized pigs: a possible source of therapeutic immunoglobulin preparations. Hybridoma. 10(6): 673-683, 1991.
  • Mahalingam, M.Ugen, KE.Kao, KJ.Klein, PA. Functional role of platelets in experimental metastasis studied with cloned murine fibrosarcoma cell variants. Cancer Research. 48(6): 1460-1464, 1988.
  • Ugen, KE.Mahalingam, M.Klein, PA.Kao, KJ. Inhibition of tumor cell-induced platelet aggregation and experimental tumor metastasis by the synthetic Gly-Arg-Gly-Asp-Ser peptide. Journal of the National Cancer Institute. 80(18): 1461-1466, 1988.

  • Positions Held
  • Professor (Medical Microbiology and Immunology, UNIVERSITY of SOUTH FLORIDA 2005 - 2006)
  • Associate Professor (Medical Microbiology and Immunology, UNIVERSITY of SOUTH FLORIDA 2000 - 2005)
  • Assistant Professor (Medical Microbiology and Immunology, UNIVERSITY of SOUTH FLORIDA 1995 - 2000)
  • Assistant Professor (Biology, UNIVERSITY OF TEXAS at SAN ANTONIO 1995 - 1995)
  • Research Assistant Professor (Pathology, UNIVERSITY OF PENNSYLVANIA 1994 - 1995)
  • Research Associate (Pathology, UNIVERSITY OF PENNSYLVANIA 1991 - 1994)
  • Research Investigator (Immunology Program, The Wistar Institute 1989 - 1991)
  • Postdoctoral Fellow (Anatomy, Jefferson Medical College 1988 - 1989)
  • Postdoctoral Fellow (Pathology, UNIVERSITY OF FLORIDA 1986 - 1988)


  • Memberships
  • American Association for Cancer Research (Member, 2007 - Present)
  • American Society for Gene Therapy (Member, 2007 - Present)


  • Profile last modified on 09/16/2013