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Gloria Ferreira,  Ph.D.
Member, Florida Center of Excellence for Biomolecular Identification and Targeted Therapeutics (FCoE-BITT)
Affiliated Professor, Department of Chemistry
Professor, Department of Molecular Medicine, College of Medicine



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Email: gferreir@health.usf.edu
Phone: (813) 974-5797
Office: MDC, 2152
Mailing address 12901 Bruce B. Downs Blvd. MDC 7
Tampa, FL 33612
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  • PHD, Biochemistry, University Of Georgia , Athens, United States - 1986.
    Interdisciplinary & Emerging Signature Programs
    Cardiovascular, Cancer Biology.

    Lab Link:  

    Selected Publications
  • Hunter, GA.Ferreira, GC. Molecular enzymology of 5-Aminolevulinate synthase, the gatekeeper of heme biosynthesis. Biochimica et biophysica acta. (): , 2011.
  • Lendrihas, T.Hunter, GA.Ferreira, GC. Targeting the active site gate to yield hyperactive variants of 5-aminolevulinate synthase. The Journal of biological chemistry. 285(18): 13704-11, 2010.
  • Hunter, GA.Ferreira, GC. Identification and characterization of an inhibitory metal ion-binding site in ferrochelatase. The Journal of biological chemistry. 285(53): 41836-42, 2010.
  • Hunter, GA.Ferreira, GC. 5-aminolevulinate synthase: catalysis of the first step of heme biosynthesis. Cellular and molecular biology (Noisy-le-Grand, France). 55(1): 102-10, 2009.
  • Lendrihas, T.Zhang, J.Hunter, GA.Ferreira, GC. Arg-85 and Thr-430 in murine 5-aminolevulinate synthase coordinate acyl-CoA-binding and contribute to substrate specificity. Protein science : a publication of the Protein Society. 18(9): 1847-59, 2009.
  • Karlberg, T.Hansson, MD.Yengo, RK.Johansson, R.Thorvaldsen, HO.Ferreira, GC.Hansson, M.Al-Karadaghi, S. Porphyrin binding and distortion and substrate specificity in the ferrochelatase reaction: the role of active site residues. Journal of molecular biology. 378(5): 1074-83, 2008.
  • Hunter, GA.Sampson, MP.Ferreira, GC. Metal ion substrate inhibition of ferrochelatase. The Journal of biological chemistry. 283(35): 23685-91, 2008.
  • Turbeville, TD.Zhang, J.Hunter, GA.Ferreira, GC. Histidine 282 in 5-aminolevulinate synthase affects substrate binding and catalysis. Biochemistry. 46(20): 5972-81, 2007.
  • Hunter, GA.Zhang, J.Ferreira, GC. Transient kinetic studies support refinements to the chemical and kinetic mechanisms of aminolevulinate synthase. The Journal of biological chemistry. 282(32): 23025-35, 2007.
  • Gakh, O.Park, S.Liu, G.Macomber, L.Imlay, JA.Ferreira, GC.Isaya, G. Mitochondrial iron detoxification is a primary function of frataxin that limits oxidative damage and preserves cell longevity. Human molecular genetics. 15(3): 467-79, 2006.
  • Al-Karadaghi, S.Franco, R.Hansson, M.Shelnutt, JA.Isaya, G.Ferreira, GC. Chelatases: distort to select? Trends in biochemical sciences. 31(3): 135-42, 2006.
  • Dias, JS.Macedo, AL.Ferreira, GC.Peterson, FC.Volkman, BF.Goodfellow, BJ. The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP. The Journal of biological chemistry. 281(42): 31553-61, 2006.
  • Shi, Z.Franco, R.Haddad, R.Shelnutt, JA.Ferreira, GC. The conserved active-site loop residues of ferrochelatase induce porphyrin conformational changes necessary for catalysis. Biochemistry. 45(9): 2904-12, 2006.
  • Shi, Z.Ferreira, GC. Modulation of inhibition of ferrochelatase by N-methylprotoporphyrin. The Biochemical journal. 399(1): 21-8, 2006.
  • Zhang, J.Cheltsov, AV.Ferreira, GC. Conversion of 5-aminolevulinate synthase into a more active enzyme by linking the two subunits: spectroscopic and kinetic properties. Protein science : a publication of the Protein Society. 14(5): 1190-200, 2005.
  • Shipovskov, S., Karlberg, T., Fodje, M., Hansson, M. D., Ferreira, G. C, Hansson, M., Reimann, C. T. and Al-Karadaghi, S. Ferrochelatase-catalyzed Metallation of the Transition-state Inhibitor N-methyl Mesoporphyrin: A Time-resolved Crystallographic and Mass Spectrometric Study. Journal of Molecular Biololgy. 352(): 1081-1090, 2005.
  • O'Neill, HA.Gakh, O.Park, S.Cui, J.Mooney, SM.Sampson, M.Ferreira, GC.Isaya, G. Assembly of human frataxin is a mechanism for detoxifying redox-active iron. Biochemistry. 44(2): 537-45, 2005.
  • Franco, R.Bai, G.Prosinecki, V.Abrunhosa, F.Ferreira, GC.Bastos, M. Porphyrin-substrate binding to murine ferrochelatase: effect on the thermal stability of the enzyme. The Biochemical journal. 386(Pt 3): 599-605, 2005.
  • Hunter, GA.Rivera, E.Ferreira, GC. Supraphysiological concentrations of 5-aminolevulinic acid dimerize in solution to produce superoxide radical anions via a protonated dihydropyrazine intermediate. Archives of biochemistry and biophysics. 437(2): 128-37, 2005.
  • Dias, JS.Macedo, AL.Ferreira, GC.Jeanty, N.Taketani, S.Goodfellow, BJ.Peterson, FC.Volkman, BF. 1H, 15N and 13C resonance assignments of the heme-binding protein murine p22HBP. Journal of biomolecular NMR. 32(4): 338, 2005.
  • Shi, Z.Ferreira, GC. Probing the active site loop motif of murine ferrochelatase by random mutagenesis. The Journal of biological chemistry. 279(19): 19977-86, 2004.
  • Ferreira, G. C. Heme Synthesis Encyclopedia of Biological Chemistry. ((Lennarz, W. and Lane, M. D., Eds.), Academic Press, San Diego, CA, USA.): 2:362-2:366, 2004.
  • Hofer, T.Wenger, RH.Kramer, MF.Ferreira, GC.Gassmann, M. Hypoxic up-regulation of erythroid 5-aminolevulinate synthase. Blood. 101(1): 348-50, 2003.
  • Park, S.Gakh, O.O'Neill, HA.Mangravita, A.Nichol, H.Ferreira, GC.Isaya, G. Yeast frataxin sequentially chaperones and stores iron by coupling protein assembly with iron oxidation. The Journal of biological chemistry. 278(33): 31340-51, 2003.
  • Shi, Z.Ferreira, GC. A continuous anaerobic fluorimetric assay for ferrochelatase by monitoring porphyrin disappearance. Analytical biochemistry. 318(1): 18-24, 2003.
  • Krishnamachary, B., Berg-Dixon, S., Kelly, B., Agani, F., Feldser, D., Ferreira, G., Iyer, N., LaRusch, J., Pak, B., Taghavi, P., and Semenza, G. L. Regulation of Colon Carcinoma Cell Invasion by Hypoxia-inducible Factor 1. Cancer Res.. 63(): 1138-1143, 2003.
  • Cheltsov, AV.Guida, WC.Ferreira, GC. Circular permutation of 5-aminolevulinate synthase: effect on folding, conformational stability, and structure. The Journal of biological chemistry. 278(30): 27945-55, 2003.
  • Zhang, J.Ferreira, GC. Transient state kinetic investigation of 5-aminolevulinate synthase reaction mechanism. The Journal of biological chemistry. 277(47): 44660-9, 2002.
  • Ferreira, GC.Cheltsov, AV. Circular permutation of 5-aminolevulinate synthase as a tool to evaluate folding, structure and function. Cellular and molecular biology (Noisy-le-Grand, France). 48(1): 11-6, 2002.
  • Lu, Y.Sousa, A.Franco, R.Mangravita, A.Ferreira, GC.Moura, I.Shelnutt, JA. Binding of protoporphyrin IX and metal derivatives to the active site of wild-type mouse ferrochelatase at low porphyrin-to-protein ratios. Biochemistry. 41(26): 8253-62, 2002.
  • Ferreira, GC.Franco, R.Mangravita, A.George, GN. Unraveling the substrate-metal binding site of ferrochelatase: an X-ray absorption spectroscopic study. Biochemistry. 41(15): 4809-18, 2002.
  • Ferreira, GC.Zhang, JS. Mechanism of 5-aminolevulinate synthase and the role of the protein environment in controlling the cofactor chemistry. Cellular and molecular biology (Noisy-le-Grand, France). 48(8): 827-33, 2002.
  • Goodfellow, BJ.Dias, JS.Ferreira, GC.Henklein, P.Wray, V.Macedo, AL. The solution structure and heme binding of the presequence of murine 5-aminolevulinate synthase. FEBS letters. 505(2): 325-31, 2001.
  • Franco, R.Pereira, AS.Tavares, P.Mangravita, A.Barber, MJ.Moura, I.Ferreira, GC. Substitution of murine ferrochelatase glutamate-287 with glutamine or alanine leads to porphyrin substrate-bound variants. The Biochemical journal. 356(Pt 1): 217-22, 2001.
  • Cheltsov, AV.Barber, MJ.Ferreira, GC. Circular permutation of 5-aminolevulinate synthase. Mapping the polypeptide chain to its function. The Journal of biological chemistry. 276(22): 19141-9, 2001.
  • Kramer, MF.Gunaratne, P.Ferreira, GC. Transcriptional regulation of the murine erythroid-specific 5-aminolevulinate synthase gene. Gene. 247(1-2): 153-66, 2000.
  • Franco, R.Ma, JG.Lu, Y.Ferreira, GC.Shelnutt, JA. Porphyrin interactions with wild-type and mutant mouse ferrochelatase. Biochemistry. 39(10): 2517-29, 2000.
  • Schneider-Yin, X.Gouya, L.Dorsey, M.Rüfenacht, U.Deybach, JC.Ferreira, GC. Mutations in the iron-sulfur cluster ligands of the human ferrochelatase lead to erythropoietic protoporphyria. Blood. 96(4): 1545-9, 2000.
  • Hunter, GA.Ferreira, GC. Pre-steady-state reaction of 5-aminolevulinate synthase. Evidence for a rate-determining product release. The Journal of biological chemistry. 274(18): 12222-8, 1999.
  • Hunter, GA.Ferreira, GC. Lysine-313 of 5-Aminolevulinate Synthase Acts as a General Base during Formation of the Quinonoid Reaction Intermediates. Biochemistry. 38(38): 12526, 1999.
  • Ferreira, GC. Ferrochelatase. The international journal of biochemistry & cell biology. 31(10): 995-1000, 1999.
  • Feldser, D., Agani, F., Iyer, N. V., Pak, B., Ferreira, G., and Semenza, G. L. Reciprocal Positive Regulation of Hypoxia-inducible Factor 1 and Insulin-like Growth Factor 2. Cancer Res.. 59(): 3915-3918, 1999.
  • Hunter, GA.Ferreira, GC. Lysine-313 of 5-aminolevulinate synthase acts as a general base during formation of the quinonoid reaction intermediates. Biochemistry. 38(12): 3711-8, 1999.
  • Gong, J.Hunter, GA.Ferreira, GC. Aspartate-279 in aminolevulinate synthase affects enzyme catalysis through enhancing the function of the pyridoxal 5'-phosphate cofactor. Biochemistry. 37(10): 3509-17, 1998.
  • Tan, D.Harrison, T.Hunter, GA.Ferreira, GC. Role of arginine 439 in substrate binding of 5-aminolevulinate synthase. Biochemistry. 37(6): 1478-84, 1998.
  • Tan, D.Barber, MJ.Ferreira, GC. The role of tyrosine 121 in cofactor binding of 5-aminolevulinate synthase. Protein science : a publication of the Protein Society. 7(5): 1208-13, 1998.
  • Gong, J.Kay, CJ.Barber, MJ.Ferreira, GC. Mutations at a glycine loop in aminolevulinate synthase affect pyridoxal phosphate cofactor binding and catalysis. Biochemistry. 35(45): 14109-17, 1996.
  • Tan, D.Ferreira, GC. Active site of 5-aminolevulinate synthase resides at the subunit interface. Evidence from in vivo heterodimer formation. Biochemistry. 35(27): 8934-41, 1996.
  • Ferreira, GC. Ferrochelatase binds the iron-responsive element present in the erythroid 5-aminolevulinate synthase mRNA. Biochemical and biophysical research communications. 214(3): 875-8, 1995.
  • Franco, R.Moura, JJ.Moura, I.Lloyd, SG.Huynh, BH.Forbes, WS.Ferreira, GC. Characterization of the iron-binding site in mammalian ferrochelatase by kinetic and Mössbauer methods. The Journal of biological chemistry. 270(44): 26352-7, 1995.
  • Ferreira, GC.Franco, R.Lloyd, SG.Moura, I.Moura, JJ.Huynh, BH. Structure and function of ferrochelatase. Journal of bioenergetics and biomembranes. 27(2): 221-9, 1995.
  • Ferreira, GC.Vajapey, U.Hafez, O.Hunter, GA.Barber, MJ. Aminolevulinate synthase: lysine 313 is not essential for binding the pyridoxal phosphate cofactor but is essential for catalysis. Protein science : a publication of the Protein Society. 4(5): 1001-6, 1995.
  • Gong, J.Ferreira, GC. Aminolevulinate synthase: functionally important residues at a glycine loop, a putative pyridoxal phosphate cofactor-binding site. Biochemistry. 34(5): 1678-85, 1995.
  • Ferreira, GC. Heme biosynthesis: biochemistry, molecular biology, and relationship to disease. Journal of bioenergetics and biomembranes. 27(2): 147-50, 1995.
  • Ferreira, GC.Gong, J. 5-Aminolevulinate synthase and the first step of heme biosynthesis. Journal of bioenergetics and biomembranes. 27(2): 151-9, 1995.
  • Hunter, GA.Ferreira, GC. A continuous spectrophotometric assay for 5-aminolevulinate synthase that utilizes substrate cycling. Analytical biochemistry. 226(2): 221-4, 1995.
  • Jabs, EW.Thomas, PJ.Bernstein, M.Coss, C.Ferreira, GC.Pedersen, PL. Chromosomal localization of genes required for the terminal steps of oxidative metabolism: alpha and gamma subunits of ATP synthase and the phosphate carrier. Human genetics. 93(5): 600-2, 1994.
  • Ferreira, GC.Franco, R.Lloyd, SG.Pereira, AS.Moura, I.Moura, JJ.Huynh, BH. Mammalian ferrochelatase, a new addition to the metalloenzyme family. The Journal of biological chemistry. 269(10): 7062-5, 1994.
  • Ferreira, GC. Mammalian ferrochelatase. Overexpression in Escherichia coli as a soluble protein, purification and characterization. The Journal of biological chemistry. 269(6): 4396-400, 1994.
  • Ferreira, GC. Erythroid 5-aminolevulinate synthase and X-linked sideroblastic anemia. The Journal of the Florida Medical Association. 80(7): 481-3, 1993.
  • Ferreira, GC.Dailey, HA. Expression of mammalian 5-aminolevulinate synthase in Escherichia coli. Overproduction, purification, and characterization. The Journal of biological chemistry. 268(1): 584-90, 1993.
  • Ferreira, GC.Neame, PJ.Dailey, HA. Heme biosynthesis in mammalian systems: evidence of a Schiff base linkage between the pyridoxal 5'-phosphate cofactor and a lysine residue in 5-aminolevulinate synthase. Protein science : a publication of the Protein Society. 2(11): 1959-65, 1993.
  • Ferreira, GC.Pedersen, PL. Phosphate transport in mitochondria: past accomplishments, present problems, and future challenges. Journal of bioenergetics and biomembranes. 25(5): 483-92, 1993.
  • Ferreira, GC.Pedersen, PL. Overexpression of higher eukaryotic membrane proteins in bacteria. Novel insights obtained with the liver mitochondrial proton/phosphate symporter. The Journal of biological chemistry. 267(8): 5460-6, 1992.
  • Pratt, RD.Ferreira, GC.Pedersen, PL. Mitochondrial phosphate transport. Import of the H+/Pi symporter and role of the presequence. The Journal of biological chemistry. 266(2): 1276-80, 1991.
  • Ferreira, GC.Pratt, RD.Pedersen, PL. Mitochondrial proton/phosphate transporter. An antibody directed against the COOH terminus and proteolytic cleavage experiments provides new insights about its membrane topology. The Journal of biological chemistry. 265(34): 21202-6, 1990.
  • Ferreira, GC.Pratt, RD.Pedersen, PL. Energy-linked anion transport. Cloning, sequencing, and characterization of a full length cDNA encoding the rat liver mitochondrial proton/phosphate symporter. The Journal of biological chemistry. 264(26): 15628-33, 1989.
  • Ferreira, GC.Dailey, HA. Mouse protoporphyrinogen oxidase. Kinetic parameters and demonstration of inhibition by bilirubin. The Biochemical journal. 250(2): 597-603, 1988.
  • Ferreira, GC.Andrew, TL.Karr, SW.Dailey, HA. Organization of the terminal two enzymes of the heme biosynthetic pathway. Orientation of protoporphyrinogen oxidase and evidence for a membrane complex. The Journal of biological chemistry. 263(8): 3835-9, 1988.
  • Ferreira, GC.Dailey, HA. Reconstitution of the two terminal enzymes of the heme biosynthetic pathway into phospholipid vesicles. The Journal of biological chemistry. 262(9): 4407-12, 1987.
  • Rigler, MW.Ferreira, GC.Patton, JS. Intramembranous particles are clustered on microvillus membrane vesicles. Biochimica et biophysica acta. 816(1): 131-41, 1985.

  • Positions Held

  • American Society for Biochemistry and Molecular Biology (Member, 2007 - Present)
  • Biophysical Society (Member, 2007 - Present)
  • Protein Society (Member, 2007 - Present)
  • American Association for Advancement of Science (Member, 2007 - Present)
  • Sociedade Portuguesa de Bioquimica (Member, 2007 - Present)
  • American Society for Hematology (Member, 2007 - Present)
  • American Porphyria Foundation (Member, 2007 - Present)
  • Society of Porphyrins & Phatlocyanines (Member, 2007 - Present)
  • American Chemical Society (Member, 2007 - Present)

  • Awards/Honors
  • Elected Member (Nominating Committee of the Division of Biological Chemistry, American Chemical Society 2002 - 2004)
  • Elected Vice-Chair (Year 2000) and Chair (Year 2002) (Gordon Research Conference, The Chemistry and Biology of Tetrapyrroles - 2000)
  • National Science Foundation Young Investigator Award (1992 - 1997)
  • Presidential Young Faculty Award (University of South Florida 1995 - 1996)

  • Profile last modified on 01/28/2014