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Gene Ness,  Ph.D.
Professor, Department of Molecular Medicine, College of Medicine
Associate Member, H. Lee Moffitt Cancer Research Institute


Primary Department: COLLEGE OF MEDICINE MOLECULAR MEDICINE

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Contacts
Email: gness@health.usf.edu
Mailing address 12901 Bruce B. Downs Blvd. MDC 7
Tampa, FL 33612
 
Education
  • PHD, Biochemistry, University Of North Dakota , Grand Forks, United States - 1971.
  • B.S. , Chemistry, Bemidji State University ,USA - 1966.
  •  
    Interdisciplinary & Emerging Signature Programs
    Cardiovascular.
     


    Selected Publications
  • Ness, GC.Nazian, SJ. Developmental expression of multiple forms of 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA in rat testes. Journal of andrology. 13(4): 318-22, .
  • Ness, GC.Lalich, JJ.Porter, JW. Stimulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase activity in allylamine-fed rats. Atherosclerosis. 19(2): 185-9, .
  • Nazian, SJ.Brewer, LD.Ness, GC. Pituitary regulation of the expression of the farnesyl pyrophosphate synthetase gene in the testes of the sexually maturing rat. Journal of andrology. 12(4): 264-72, .
  • You, M.Cao, Q.Liang, X.Ajmo, JM.Ness, GC. Mammalian sirtuin 1 is involved in the protective action of dietary saturated fat against alcoholic fatty liver in mice. The Journal of nutrition. 138(3): 497-501, 2008.
  • You, M.Liang, X.Ajmo, JM.Ness, GC. Involvement of mammalian sirtuin 1 in the action of ethanol in the liver. American journal of physiology. Gastrointestinal and liver physiology. 294(4): G892-8, 2008.
  • Lagor, WR.Heller, R.de Groh, ED.Ness, GC. Functional analysis of the hepatic HMG-CoA reductase promoter by in vivo electroporation. Experimental biology and medicine (Maywood, N.J.). 232(3): 353-61, 2007.
  • Lopez, D.Abisambra Socarrás, JF.Bedi, M.Ness, GC. Activation of the hepatic LDL receptor promoter by thyroid hormone. Biochimica et biophysica acta. 1771(9): 1216-25, 2007.
  • Ness, GC.Holland, RC.Lopez, D. Selective compensatory induction of hepatic HMG-CoA reductase in response to inhibition of cholesterol absorption. Experimental biology and medicine (Maywood, N.J.). 231(5): 559-65, 2006.
  • Lopez, D.Ness, GC. Characterization of the rat LDL receptor 5'-flanking region. Biochimica et biophysica acta. 1761(4): 492-500, 2006.
  • Ness, GC.Holland, RC. Degradation of HMG-CoA reductase in rat liver is cholesterol and ubiquitin independent. FEBS letters. 579(14): 3126-30, 2005.
  • Ness, GC.Pendleton, LC.McCreery, MJ. Target size analysis by radiation inactivation: the use of free radical scavengers. Experimental biology and medicine (Maywood, N.J.). 230(7): 455-63, 2005.
  • Lagor, WR.de Groh, ED.Ness, GC. Diabetes alters the occupancy of the hepatic 3-hydroxy-3-methylglutaryl-CoA reductase promoter. The Journal of biological chemistry. 280(44): 36601-8, 2005.
  • Ness, GC.Gertz, KR. Hepatic HMG-CoA reductase expression and resistance to dietary cholesterol. Experimental biology and medicine (Maywood, N.J.). 229(5): 412-6, 2004.
  • Ness, GC.Gertz, KR. Increased sensitivity to dietary cholesterol in diabetic and hypothyroid rats associated with low levels of hepatic HMG-CoA reductase expression. Experimental biology and medicine (Maywood, N.J.). 229(5): 407-11, 2004.
  • Osborne, AR.Pollock, VV.Lagor, WR.Ness, GC. Identification of insulin-responsive regions in the HMG-CoA reductase promoter. Biochemical and biophysical research communications. 318(4): 814-8, 2004.
  • Oettl, K.Höfler, G.Ness, GC.Sattler, W.Malle, E. An apparent decrease in cholesterol biosynthesis in peroxisomal-defective Chinese hamster ovary cells is related to impaired mitochondrial oxidation. Biochemical and biophysical research communications. 305(4): 957-63, 2003.
  • Ness, GC.Kohlruss, N.Gertz, KR. Association of the low-density lipoprotein receptor with caveolae in hamster and rat liver. Biochemical and biophysical research communications. 303(1): 177-81, 2003.
  • Ness, GC.Gertz, KR.Holland, RC. Regulation of hepatic lanosterol 14 alpha-demethylase gene expression by dietary cholesterol and cholesterol-lowering agents. Archives of biochemistry and biophysics. 395(2): 233-8, 2001.
  • Fitzky, BU.Moebius, FF.Asaoka, H.Waage-Baudet, H.Xu, L.Xu, G.Maeda, N.Kluckman, K.Hiller, S.Yu, H.Batta, AK.Shefer, S.Chen, T.Salen, G.Sulik, K.Simoni, RD.Ness, GC.Glossmann, H.Patel, SB.Tint, GS. 7-Dehydrocholesterol-dependent proteolysis of HMG-CoA reductase suppresses sterol biosynthesis in a mouse model of Smith-Lemli-Opitz/RSH syndrome. The Journal of clinical investigation. 108(6): 905-15, 2001.
  • Ness, GC.Chambers, CM. Feedback and hormonal regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase: the concept of cholesterol buffering capacity. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 224(1): 8-19, 2000.
  • Ness, GC.Chambers, CM.Lopez, D. Atorvastatin action involves diminished recovery of hepatic HMG-CoA reductase activity. Journal of lipid research. 39(1): 75-84, 1998.
  • Lopez, D.Chambers, CM.Keller, RK.Ness, GC. Compensatory responses to inhibition of hepatic squalene synthase. Archives of biochemistry and biophysics. 351(2): 159-66, 1998.
  • Chambers, CM.Ness, GC. Dietary cholesterol regulates hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression in rats primarily at the level of translation. Archives of biochemistry and biophysics. 354(2): 317-22, 1998.
  • Ness, GC.Lopez, D.Chambers, CM.Newsome, WP.Cornelius, P.Long, CA.Harwood, HJ. Effects of L-triiodothyronine and the thyromimetic L-94901 on serum lipoprotein levels and hepatic low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and apo A-I gene expression. Biochemical pharmacology. 56(1): 121-9, 1998.
  • Ness, GC.Lopez, D.Chambers, CM.Zhao, Z.Beach, DL.Ko, SS.Trzaskos, JM. Effects of 15-oxa-32-vinyl-lanost-8-ene-3 beta,32 diol on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase and low density lipoprotein receptor in rat liver. Archives of biochemistry and biophysics. 357(2): 259-64, 1998.
  • Salen, G.Shefer, S.Nguyen, L.Ness, GC.Tint, GS.Batta, AK. Sitosterolemia. Sub-cellular biochemistry. 28(): 453-76, 1997.
  • Ness, GC.Lopez, D.Borrego, O.Gilbert-Barness, E. Increased expression of low-density lipoprotein receptors in a Smith-Lemli-Opitz infant with elevated bilirubin levels. American journal of medical genetics. 68(3): 294-9, 1997.
  • Chambers, CM.McLean, MP.Ness, GC. Smith-Lemli-Opitz syndrome produced in rats with AY 9944 treated by intravenous injection of lipoprotein cholesterol. American journal of medical genetics. 68(3): 322-7, 1997.
  • Lopez, D.Chambers, CM.Ness, GC. 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors unmask cryptic regulatory mechanisms. Archives of biochemistry and biophysics. 343(1): 118-22, 1997.
  • Lopez, D.Ness, GC. Inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase unmask transcriptional regulation of hepatic low-density lipoprotein receptor gene expression by dietary cholesterol. Archives of biochemistry and biophysics. 344(1): 215-9, 1997.
  • Chambers, CM.Ness, GC. Translational regulation of hepatic HMG-CoA reductase by dietary cholesterol. Biochemical and biophysical research communications. 232(2): 278-81, 1997.
  • Keller, RK.Zhao, Z.Chambers, C.Ness, GC. Farnesol is not the nonsterol regulator mediating degradation of HMG-CoA reductase in rat liver. Archives of biochemistry and biophysics. 328(2): 324-30, 1996.
  • Ness, GC.Chambers, CM. The diurnal variation of hepatic HMG-CoA reductase activity is due to changes in the level of immunoreactive protein. Archives of biochemistry and biophysics. 327(1): 41-4, 1996.
  • Ness, GC.Zhao, Z.Lopez, D. Inhibitors of cholesterol biosynthesis increase hepatic low-density lipoprotein receptor protein degradation. Archives of biochemistry and biophysics. 325(2): 242-8, 1996.
  • Xu, G.Salen, G.Shefer, S.Ness, GC.Nguyen, LB.Tint, GS.Parker, TS.Roberts, J.Batta, AK.Chen, TS.Zhao, Z.Kong, X. Increasing hepatic cholesterol 7alpha-hydroxylase reduces plasma cholesterol concentrations in normocholesterolemic and hypercholesterolemic rabbits. Hepatology (Baltimore, Md.). 24(4): 882-7, 1996.
  • Xu, G.Salen, G.Shefer, S.Ness, GC.Chen, TS.Zhao, Z.Salen, L.Tint, GS. Treatment of the cholesterol biosynthetic defect in Smith-Lemli-Opitz syndrome reproduced in rats by BM 15.766. Gastroenterology. 109(4): 1301-7, 1995.
  • Xu, G.Salen, G.Shefer, S.Ness, GC.Nguyen, LB.Parker, TS.Chen, TS.Zhao, Z.Donnelly, TM.Tint, GS. Unexpected inhibition of cholesterol 7 alpha-hydroxylase by cholesterol in New Zealand white and Watanabe heritable hyperlipidemic rabbits. The Journal of clinical investigation. 95(4): 1497-504, 1995.
  • Xu, G.Salen, G.Shefer, S.Ness, GC.Chen, TS.Zhao, Z.Tint, GS. Reproducing abnormal cholesterol biosynthesis as seen in the Smith-Lemli-Opitz syndrome by inhibiting the conversion of 7-dehydrocholesterol to cholesterol in rats. The Journal of clinical investigation. 95(1): 76-81, 1995.
  • Ness, GC.Lopez, D. Transcriptional regulation of rat hepatic low-density lipoprotein receptor and cholesterol 7 alpha hydroxylase by thyroid hormone. Archives of biochemistry and biophysics. 323(2): 404-8, 1995.
  • Ness, GC.Wiggins, L.Zhao, Z. Insulin increases hepatic 3-hydroxy-methylglutaryl coenzyme A reductase mRNA and immunoreactive protein levels in diabetic rats. Archives of biochemistry and biophysics. 309(1): 193-4, 1994.
  • Ness, GC.Zhao, Z.Keller, RK. Effect of squalene synthase inhibition on the expression of hepatic cholesterol biosynthetic enzymes, LDL receptor, and cholesterol 7 alpha hydroxylase. Archives of biochemistry and biophysics. 311(2): 277-85, 1994.
  • Nguyen, LB.Shefer, S.Salen, G.Ness, GC.Batta, A.Chowdhary, IR.Paroulek, E.Hauser, S. Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in the rat ileum: effects of bile acids and lovastatin. Metabolism: clinical and experimental. 43(11): 1446-50, 1994.
  • Ness, GC.Pendelton, LC.Zhao, Z. Thyroid hormone rapidly increases cholesterol 7 alpha-hydroxylase mRNA levels in hypophysectomized rats. Biochimica et biophysica acta. 1214(3): 229-33, 1994.
  • Salen, G.Batta, AK.Tint, GS.Shefer, S.Ness, GC. Inverse relationship between plasma cholestanol concentrations and bile acid synthesis in sitosterolemia. Journal of lipid research. 35(10): 1878-87, 1994.
  • Walker, RF.Ness, GC.Zhao, Z.Bercu, BB. Effects of stimulated growth hormone secretion on age-related changes in plasma cholesterol and hepatic low density lipoprotein messenger RNA concentrations. Mechanisms of ageing and development. 75(3): 215-26, 1994.
  • Ness, GC.Eales, S.Lopez, D.Zhao, Z. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by sterols and nonsterols in rat liver. Archives of biochemistry and biophysics. 308(2): 420-5, 1994.
  • Ness, GC. Developmental regulation of the expression of genes encoding proteins involved in cholesterol homeostasis. American journal of medical genetics. 50(4): 355-7, 1994.
  • Shefer, S.Salen, G.Bullock, J.Nguyen, LB.Ness, GC.Vhao, Z.Belamarich, PF.Chowdhary, I.Lerner, S.Batta, AK. The effect of increased hepatic sitosterol on the regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase and cholesterol 7 alpha-hydroxylase in the rat and sitosterolemic homozygotes. Hepatology (Baltimore, Md.). 20(1 Pt 1): 213-9, 1994.
  • Ness, GC.Zhao, Z. Thyroid hormone rapidly induces hepatic LDL receptor mRNA levels in hypophysectomized rats. Archives of biochemistry and biophysics. 315(1): 199-202, 1994.
  • Ness, GC.Zhao, Z.Wiggins, L. Insulin and glucagon modulate hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity by affecting immunoreactive protein levels. The Journal of biological chemistry. 269(46): 29168-72, 1994.
  • Keller, RK.Cannons, A.Vilsaint, F.Zhao, Z.Ness, GC. Identification and regulation of rat squalene synthetase mRNA. Archives of biochemistry and biophysics. 302(1): 304-6, 1993.
  • Xu, G.Salen, G.Shefer, S.Batta, AK.Ness, GC.Nguyen, LB.Zhao, Z.Chen, TS.Niemann, W.Tint, GS. Different feedback regulation of hepatic cholesterol and bile acid synthesis by glycodeoxycholic acid in rabbits. Gastroenterology. 105(4): 1192-9, 1993.
  • Xu, G.Salen, G.Batta, AK.Shefer, S.Nguyen, LB.Niemann, W.Chen, TS.Arora-Mirchandani, R.Ness, GC.Tint, GS. Glycocholic acid and glycodeoxycholic acid but not glycoursocholic acid inhibit bile acid synthesis in the rabbit. Gastroenterology. 102(5): 1717-23, 1992.
  • Salen, G.Shefer, S.Nguyen, L.Ness, GC.Tint, GS.Shore, V. Sitosterolemia. Journal of lipid research. 33(7): 945-55, 1992.
  • Shefer, S.Nguyen, LB.Salen, G.Ness, GC.Chowdhary, IR.Lerner, S.Batta, AK.Tint, GS. Differing effects of cholesterol and taurocholate on steady state hepatic HMG-CoA reductase and cholesterol 7 alpha-hydroxylase activities and mRNA levels in the rat. Journal of lipid research. 33(8): 1193-200, 1992.
  • Ness, GC. Thyroid hormone. Basis for its hypocholesterolemic effect. The Journal of the Florida Medical Association. 78(6): 383-5, 1991.
  • Ness, GC.Keller, RK.Pendleton, LC. Feedback regulation of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase activity by dietary cholesterol is not due to altered mRNA levels. The Journal of biological chemistry. 266(23): 14854-7, 1991.
  • Shefer, S.Nguyen, LB.Salen, G.Ness, GC.Tint, GS.Batta, AK.Hauser, S.Rani, I. Regulation of cholesterol 7 alpha-hydroxylase by hepatic 7 alpha-hydroxylated bile acid flux and newly synthesized cholesterol supply. The Journal of biological chemistry. 266(5): 2693-6, 1991.
  • Ness, GC.Pendleton, LC. Thyroid hormone increases glyceraldehyde 3-phosphate dehydrogenase gene expression in rat liver. FEBS letters. 288(1-2): 21-2, 1991.
  • Nguyen, LB.Cobb, M.Shefer, S.Salen, G.Ness, GC.Tint, GS. Regulation of cholesterol biosynthesis in sitosterolemia: effects of lovastatin, cholestyramine, and dietary sterol restriction. Journal of lipid research. 32(12): 1941-8, 1991.
  • Ness, GC.Pendleton, LC.Li, YC.Chiang, JY. Effect of thyroid hormone on hepatic cholesterol 7 alpha hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthetase and apolipoprotein A-I mRNA levels in hypophysectomized rats. Biochemical and biophysical research communications. 172(3): 1150-6, 1990.
  • Nguyen, LB.Shefer, S.Salen, G.Ness, GC.Tint, GS.Zaki, FG.Rani, I. A molecular defect in hepatic cholesterol biosynthesis in sitosterolemia with xanthomatosis. The Journal of clinical investigation. 86(3): 923-31, 1990.
  • Nguyen, LB.Salen, G.Shefer, S.Tint, GS.Shore, V.Ness, GC. Decreased cholesterol biosynthesis in sitosterolemia with xanthomatosis: diminished mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and enzyme protein associated with increased low-density lipoprotein receptor function. Metabolism: clinical and experimental. 39(4): 436-43, 1990.
  • Ness, GC.Sukalski, KA.Sample, CE.Pendleton, LC.McCreery, MJ.Nordlie, RC. Radiation inactivation analysis of rat liver microsomal glucose-6-phosphatase. The Journal of biological chemistry. 264(13): 7111-4, 1989.
  • Simonet, WS.Ness, GC. Post-transcriptional regulation of 3-hydroxy-3-methylglutaryl-CoA reductase mRNA in rat liver. Glucocorticoids block the stabilization caused by thyroid hormones. The Journal of biological chemistry. 264(1): 569-73, 1989.
  • Simonet, WS.Ness, GC. Transcriptional and posttranscriptional regulation of rat hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase by thyroid hormones. The Journal of biological chemistry. 263(25): 12448-53, 1988.
  • Ness, GC.Pendleton, LC.McCreery, MJ. In situ determination of the functional size of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase by radiation inactivation analysis. Biochimica et biophysica acta. 953(3): 361-4, 1988.
  • Simonet, WS.Ness, GC. Turnover rates of HMG-CoA reductase mRNA: role of pituitary and thyroid hormones. Biochemical and biophysical research communications. 146(3): 1033-9, 1987.
  • Sample, CE.Pendleton, LC.Ness, GC. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA levels by L-triiodothyronine. Biochemistry. 26(3): 727-31, 1987.
  • Harwood, HJ.Alvarez, IM.Greene, YJ.Ness, GC.Stacpoole, PW. Development of a noncompetitive, solid phase, bridged biotin-avidin enzyme immunoassay for measurement of human leukocyte microsomal HMG-CoA reductase protein concentration. Journal of lipid research. 28(3): 292-304, 1987.
  • Ness, GC.Pendleton, LC.Pendleton, AS. Loss of NADPH during assays of HMG-CoA reductase: implications and approaches to minimize errors. Lipids. 22(6): 409-12, 1987.
  • Eichler, DC.Solomonson, LP.Barber, MJ.McCreery, MJ.Ness, GC. Radiation inactivation analysis of enzymes. Effect of free radical scavengers on apparent target sizes. The Journal of biological chemistry. 262(20): 9433-6, 1987.
  • Sample, CE.Ness, GC. Regulation of the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase by insulin. Biochemical and biophysical research communications. 137(1): 201-7, 1986.
  • Ness, GC.Sample, CE.Smith, M.Pendleton, LC.Eichler, DC. Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase. The Biochemical journal. 233(1): 167-72, 1986.
  • Ness, GC.McCreery, MJ.Sample, CE.Smith, M.Pendleton, LC. Sulfhydryl/disulfide forms of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase. The Journal of biological chemistry. 260(30): 16395-9, 1985.
  • Ness, GC.Eales, SJ.Pendleton, LC.Smith, M. Activation of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase by NADPH. Effects of dietary treatments. The Journal of biological chemistry. 260(23): 12391-3, 1985.
  • Singer, II.Kawka, DW.Kazazis, DM.Alberts, AW.Chen, JS.Huff, JW.Ness, GC. Hydroxymethylglutaryl-coenzyme A reductase-containing hepatocytes are distributed periportally in normal and mevinolin-treated rat livers. Proceedings of the National Academy of Sciences of the United States of America. 81(17): 5556-60, 1984.
  • Phillips, CE.Ness, GC. Topography of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase. Biochemical and biophysical research communications. 119(2): 772-8, 1984.
  • Ness, GC. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Molecular and cellular biochemistry. 53-54(1-2): 299-306, 1983.
  • Ness, GC.Benton, GA.Deiter, SA.Wickham, PS. Influence of mevalonate kinase on studies of the MgATP-dependent inactivator of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Archives of biochemistry and biophysics. 214(2): 705-13, 1982.
  • Ness, GC.Way, SC.Wickham, PS. Proteinase involvement in the solubilization of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Biochemical and biophysical research communications. 102(1): 81-5, 1981.
  • Ness, GC.Spindler, CD.Benton, GA. Characteristics of magnesium-adenosine triphosphate-dependent inactivators of 3-hydroxy-3-methylglutaryl coenzyme A reductase. The Journal of biological chemistry. 255(19): 9013-6, 1980.
  • Keller, RK.Adair, WL.Ness, GC. Studies on the regulation of glycoprotein biosynthesis. An investigation of the rate-limiting steps of dolichyl phosphate biosynthesis. The Journal of biological chemistry. 254(20): 9966-9, 1979.
  • Ness, GC.Spindler, CD.Moffler, MH. Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase from rat liver. Archives of biochemistry and biophysics. 197(2): 493-9, 1979.
  • Ness, GC.Moffler, MH. Influence of a naturally occurring competing enzymic activity on studies of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Biochimica et biophysica acta. 572(2): 333-44, 1979.
  • Ness, GC.Miller, JP.Moffler, MH.Woods, LS.Harris, HB. Perinatal development of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in rat lung, liver and brain. Lipids. 14(5): 447-50, 1979.
  • Slakey, LL.Ferrick, TJ.Ness, GC.Porter, JW. De novo fatty acid synthesis and fatty acid elongation catalyzed by subcellular fractions from hog and human aorta. Lipids. 14(5): 451-7, 1979.
  • Ness, GC.Moffler, MH. Interference with the determination of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and its properties by a microsomal enzymic activity. Archives of biochemistry and biophysics. 189(1): 221-3, 1978.
  • Lakshmanan, MR.Dugan, RE.Nepokroeff, CM.Ness, GC.Porter, JW. Regulation of rat liver beta-hydroxy-beta-methylglutaryl coenzyme. A reductase activity and cholesterol levels of serum and liver in various dietary and hormonal states. Archives of biochemistry and biophysics. 168(1): 89-95, 1975.
  • Nepokroeff, CM.Lakshmanan, MR.Ness, GC.Muesing, RA.Kleinsek, DA.Porter, JW. Coordinate control of rat liver lipogenic enzymes by insulin. Archives of biochemistry and biophysics. 162(2): 340-4, 1974.
  • Dugan, RE.Ness, GC.Lakshmanan, MR.Nepokroeff, CM.Porter, JW. Regulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase by the interplay of hormones. Archives of biochemistry and biophysics. 161(2): 499-504, 1974.
  • Nepokroeff, CM.Lakshmanan, MR.Ness, GC.Dugan, RE.Porter, JW. Regulation of the diurnal rhythm of rat liver beta-hydroxy-beta-methylglutaryl coenzmye A reductase activity by insulin, glucagon, cyclic AMP and hydrocortisone. Archives of biochemistry and biophysics. 160(2): 387-96, 1974.
  • Lakshmanan, MR.Nepokroeff, CM.Ness, GC.Dugan, RE.Porter, JW. Stimulation by insulin of rat liver -hydroxy- -methylglutaryl coenzyme A reductase and cholesterol-synthesizing activities. Biochemical and biophysical research communications. 50(3): 704-10, 1973.
  • Ness, GC.Dugan, RE.Lakshmanan, MR.Nepokroeff, CM.Porter, JW. Stimulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase activity in hypophysectomized rats by L-triiodothyronine. Proceedings of the National Academy of Sciences of the United States of America. 70(12): 3839-42, 1973.
  • Slakey, LL.Ness, GC.Qureshi, N.Porter, JW. Occurrence of the enzymes effecting the conversion of acetyl CoA to squalene in homogenates of hog aorta. Journal of lipid research. 14(4): 485-94, 1973.
  • Ness, GC.Takahashi, T.Lee, YP. Thyroid hormones on amino acid and protein metabolism. I. Concentration and composition of free amino acids in blood plasma of the rat. Endocrinology. 85(6): 1166-71, 1969.

  • Positions Held
  • Professor (Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida 1986 - 2006)
  • Member (Institute for Biomolecular Science, University of South Florida 1986 - 2002)
  • Associate Professor (Department of Biochemistry, College of Medicine, University of South Florida 1980 - 1986)
  • Assistant Professor (Department of Biochemistry, College of Medicine, University of South Florida 1974 - 1980)


  • Memberships
  • Society for Experimental Biology and Medicine (Member, 2007 - Present)
  • American Heart Association (Member, 2007 - Present)
  • American Society of Biochemistry and Molecular Biology (Member, 2007 - Present)


  • Awards/Honors
  • Mentor, Outstanding Dissertation Prize Winning Student ( - 1999)
  • Professorial Excellence Award ( - 1997)
  • Bronze Service Award (American Heart Association - Florida Affiliate - 1992)
  • Outstanding Scientist Medal (Florida Academy of Sciences - 1988)


  • Lectures
  • "What is next in Lowering Cholesterol and Reducing the Risk of a Cardiovascular Event?" Panel Discussion Leader, Second Annual Cardiovascular Signature Program Research Symposium, Advances in the Management of Cardiovascular Disease, USF. FL, United States - 2008.
  • "Statins and Beyond: Strategies for Improving Cardiovascular Health" Cardiovascular Signature Research Program Seminar Series, USF. FL, United States - 2007.
  • "Physiological Regulation of Hepatic HMG-CoA Reductase by Insulin and Thyroid Hormone" Second Annual Faculty Research Symposium, Department of Molecular Medicine, USF. FL, United States - 2007.
  • "Insulin and Thyroid Hormone Stimulate Transcription of Hepatic 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase" First Annual Cardiovascular Signature Program Research Symposium, USF. FL, United States - 2007.
  • "Physiological Regulation of Hepatic HMG-CoA Reductase" Department of Biochemistry, School of Medicine, University of North Dakota. ND, United States - 2006.
  • "Analysis of the Hepatic HMG-CoA Reductase Promoter" First Annual Faculty Research Symposium, Department of Molecular Medicine, USF. FL, United States - 2006.
  • "New Lipid Guidelines and Cardiovascular Health" Deans Lecture, School of Medicine, University of North Dakota. ND, United States - 2006.

  • Profile last modified on 03/19/2012