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Diane Allen-Gipson,  Assistant Professor of Pharmaceutical Sciences Department
Affiliate Faculty Member, Moffitt Cancer Research Molecular Oncology Affiliate
Adjunct Assistant Professor, Dept of Internal Medicine, Division of Pulmonary, Critical Care, Sleep and Allergy Medicine


Primary Department: COP DEPT OF PHARMACEUTICAL SCIENCE

Assistant Professor
COLLEGE OF MEDICINE INTERNAL MEDICINE

Assistant Professor
COP DEPT OF PHARMACEUTICAL SCIENCE

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Contacts
Email: dallengi@health.usf.edu
Phone: (813) 974-7225
Office: MDC, 3115A
Mailing address 12901 Bruce B. Downs Blvd., MDC 30
Tampa, FL 33612
 
Education
  • PhD, Pharmacy, Florida A&M University , Tallahassee, United States - 2000.
  •  
    Interdisciplinary & Emerging Signature Programs
    Women & Children's Health, Other.
     
    Research Summary

    Research Focus: Exposure to cigarette smoke is responsible for a considerable amount of pulmonary diseases including chronic bronchitis, emphysema, carcinoma of the lung, and chronic obstructive pulmonary disease (COPD) and excessive alcohol consumption also contributes to the comorbidities associated with these diseases. COPD is the only chronic disease growing in mortality in the USA and afflicts over 23 million people where at least 12 million remains undiagnosed. Furthermore, veterans are much more likely than non-veterans to have ever smoked (74% compared to 48%). By estimation of the US Department of Veteran Affairs, chronic inflammatory disorder, including asthma and COPD, is a common problem among veterans, accounting for ∼ 16% of VA hospital admissions and a third of admissions to all VA medical services, driving the need to develop novel research strategies to address the pathogenesis of these disorders. Our lab focuses on mechanisms controlling repair of bronchial epithelium particularly, adenosine signal pathway and its role of epithelial injury in airway diseases associated with cigarette smoke and/or in combination with alcohol exposure. Adenosine, an endogenous nucleoside has been recognized as an important modulator of hypoxic, ischemic and inflammatory process critical to both tissue homeostasis and tissue injury. However, the mechanisms by which adenosine-mediated airway wound healing and repair occurs after exposure to injurious agents like cigarette smoke has not been investigated. In addition to understanding the mechanisms involved in adenosine-mediated wound repair our lab has a growing interest in targeting efforts to reduce existing disparities in mortality rates particularly associated with smoke-related lung diseases and lung cancer. We recognized mortality from all causes is higher for persons with fewer years of education and for minorities including but not limited to Black and Hispanic population still remains unknown, which diseases contribute most to these disparities. Laboratory Activities: We are interested in understanding the dynamics of the receptor-mediated action of adenosine as well as investigating adenosines mechanistic signaling pathways associated with ciliary motility and clearance, pro-and- anti-inflammatory cytokine production, cell adhesion and remodeling and wound repair under conditions of cigarette smoke and/or alcohol exposure, and oxidative stress. In addition, our lab will focus on developing new therapeutic drug target and delivery systems as it relates to airway injury and repair via adenosine signaling pathway. We believe the distribution and varied effects of adenosine receptors in the epithelial airway could have for the most part, important implication in understanding chronic inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD) and smoke-related lung cancer. Methodology incorporated in our lab includes but not limited to: • Receptor-Ligand binding Studies • 3H Thymidine Incorporation Studies • HPLC • Molecular Biology Techniques i.e., Western Blots, immunoprecipations assays • Genetic manipulations (Transfections) • Ciliary Motility Studies (SAVA) • Kinase Studies (PKA, PKC and PKG) • Small animal studies (In vivo and Ex vivo)

     


    Selected Publications
  • Fukumoto, J.Fukumoto, I.Parthasarathy, PT.Cox, R.Huynh, B.Ramanathan, GK.Venugopal, RB.Allen-Gipson, DS.Lockey, RF.Kolliputi, N. NLRP3 Deletion Protects from Hyperoxia-Induced Acute Lung Injury. American journal of physiology. Cell physiology. (): , 2013.
  • Allen-Gipson, DS.Zimmerman, MC.Zhang, H.Castellanos, G.O'Malley, JK.Alvarez-Ramirez, H.Kharbanda, K.Sisson, JH.Wyatt, TA. Smoke extract impairs adenosine wound healing. American journal of respiratory cell and molecular biology. 48(5): 665-73, 2013.
  • Simet, SM.Wyatt, TA.DeVasure, J.Yanov, D.Allen-Gipson, D.Sisson, JH. Alcohol increases the permeability of airway epithelial tight junctions in Beas-2B and NHBE cells. Alcoholism, clinical and experimental research. 36(3): 432-42, 2012.
  • Allen-Gipson, DS.Blackburn, MR.Schneider, DJ.Zhang, H.Bluitt, DL.Jarrell, JC.Yanov, D.Sisson, JH.Wyatt, TA. Adenosine activation of A(2B) receptor(s) is essential for stimulated epithelial ciliary motility and clearance. American journal of physiology. Lung cellular and molecular physiology. 301(2): L171-80, 2011.
  • Allen-Gipson, DS.Jarrell, JC.Bailey, KL.Robinson, JE.Kharbanda, KK.Sisson, JH.Wyatt, TA. Ethanol blocks adenosine uptake via inhibiting the nucleoside transport system in bronchial epithelial cells. Alcoholism, clinical and experimental research. 33(5): 791-8, 2009.
  • Poole, JA.Alexis, NE.Parks, C.MacInnes, AK.Gentry-Nielsen, MJ.Fey, PD.Larsson, L.Allen-Gipson, D.Von Essen, SG.Romberger, DJ. Repetitive organic dust exposure in vitro impairs macrophage differentiation and function. The Journal of allergy and clinical immunology. 122(2): 375-82, 382.e1-4, 2008.
  • Allen-Gipson, DS.Spurzem, K.Kolm, N.Spurzem, JR.Wyatt, TA. Adenosine promotion of cellular migration in bronchial epithelial cells is mediated by the activation of cyclic adenosine monophosphate-dependent protein kinase A. Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 55(7): 378-85, 2007.
  • Slager, RE.Allen-Gipson, DS.Sammut, A.Heires, A.DeVasure, J.Von Essen, S.Romberger, DJ.Wyatt, TA. Hog barn dust slows airway epithelial cell migration in vitro through a PKCalpha-dependent mechanism. American journal of physiology. Lung cellular and molecular physiology. 293(6): L1469-74, 2007.
  • Allen-Gipson, DS.Wong, J.Spurzem, JR.Sisson, JH.Wyatt, TA. Adenosine A2A receptors promote adenosine-stimulated wound healing in bronchial epithelial cells. American journal of physiology. Lung cellular and molecular physiology. 290(5): L849-55, 2006.
  • Allen-Gipson, DS.Floreani, AA.Heires, AJ.Sanderson, SD.MacDonald, RG.Wyatt, TA. Cigarette smoke extract increases C5a receptor expression in human bronchial epithelial cells. The Journal of pharmacology and experimental therapeutics. 314(1): 476-82, 2005.
  • Allen-Gipson, DS.Romberger, DJ.Forget, MA.May, KL.Sisson, JH.Wyatt, TA. IL-8 inhibits isoproterenol-stimulated ciliary beat frequency in bovine bronchial epithelial cells. Journal of aerosol medicine : the official journal of the International Society for Aerosols in Medicine. 17(2): 107-15, 2004.
  • Floreani, AA.Wyatt, TA.Stoner, J.Sanderson, SD.Thompson, EG.Allen-Gipson, D.Heires, AJ. Smoke and C5a induce airway epithelial intercellular adhesion molecule-1 and cell adhesion. American journal of respiratory cell and molecular biology. 29(4): 472-82, 2003.
  • Heiman, AS.Allen-Gipson, D. Cytokines potentiate human eosinophil superoxide generation in the presence of N(omega)-nitro-L-arginine methyl ester. International journal of immunopharmacology. 22(2): 171-81, 2000.
  • Allen-Gipson, DS.Chen, M.Heiman, AS. Regulation of 10P2 murine mast cell proliferation and secretory function by stem cell factor or IL-9. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 217(4): 439-44, 1998.

  • Positions Held


    Profile last modified on 06/06/2013